The celestial sign in the Anglo-Saxon chronicle in the 770s: insights on contemporary solar activity

The anomalous concentration of radiocarbon in 774/775 attracted intense discussion on its origin, including the possible extreme solar event(s) exceeding any events in observational history. Anticipating such extreme solar events, auroral records were also surveyed in historical documents and those including the red celestial sign after sunset in the Anglo-Saxon Chronicle (ASC) were subjected to consideration. Usoskin et al. (2013, Astron. Astrophys. 55, L3: U13) interpreted this record as an aurora and suggested enhanced solar activity around 774/775. Conversely, Neuhäuser and Neuhäuser (2015a, 2015b, Astron. Nachr. 336, 225; 336, 913: N15a and N15b) interpreted "after sunset" as during sunset or twilight; they considered this sign as a halo display and suggested a solar minimum around 774. However, so far these records have not been discussed in comparison with eyewitness auroral records during the known extreme space-weather events, although they were discussed in relationship with potential extreme events in 774/775. Therefore, we reconstruct the observational details based on the original records in the ASC and philological references, compare them with eyewitness auroral observations during known extreme space-weather events, and consider contemporary solar activity. We clarify the observation was indeed "after sunset", reject the solar halo hypothesis, define the observational time span between 25 March 775 and 25 December 777, and note that the parallel “drawing of lunar halo display” in 806 in the ASC shown in N15b was not based on the original observation in England. We show examples of eyewitness auroral observations during twilight in known space-weather events, and this celestial sign does not contradict the observational evidence. Accordingly, we consider this event happened after the onset of the event in 774/775, but shows relatively enhanced solar activity, with other historical auroral records in the mid-770s, as also confirmed by the Be data from ice cores

Chiral patterns arising from electrostatic growth models

Recently, unusual and strikingly beautiful seahorse-like growth patterns have been observed under conditions of quasi-two-dimensional growth. These `S'-shaped patterns strongly break two-dimensional inversion symmetry; however such broken symmetry occurs only at the level of overall morphology, as the clusters are formed from achiral molecules with an achiral unit cell. Here we describe a mechanism which gives rise to chiral growth morphologies without invoking microscopic chirality. This mechanism involves trapped electrostatic charge on the growing cluster, and the enhancement of growth in regions of large electric field. We illustrate the mechanism with a tree growth model, with a continuum model for the motion of the one-dimensional boundary, and with microscopic Monte Carlo simulations. Our most dramatic results are found using the continuum model, which strongly exhibits spontaneous chiral symmetry breaking, and in particular finned `S' shapes like those seen in the experiments.Comment: RevTeX, 12 pages, 9 figure

The Prosensory Function of Sox2 in the Chicken Inner Ear Relies on the Direct Regulation of Atoh1

The proneural gene Atoh1 is crucial for the development of inner ear hair cells and it requires the function of the transcription factor Sox2 through yet unknown mechanisms. In the present work, we used the chicken embryo and HEK293T cells to explore the regulation of Atoh1 by Sox2. The results show that hair cells derive from Sox2-positive otic progenitors and that Sox2 directly activates Atoh1 through a transcriptional activator function that requires the integrity of Sox2 DNA binding domain. Atoh1 activation depends on Sox transcription factor binding sites (SoxTFBS) present in the Atoh1 3′ enhancer where Sox2 directly binds, as shown by site directed mutagenesis and chromatin immunoprecipitation (ChIP). In the inner ear, Atoh1 enhancer activity is detected in the neurosensory domain and it depends on Sox2. Dominant negative competition (Sox2HMG-Engrailed) and mutation of the SoxTFBS abolish the reporter activity in vivo. Moreover, ChIP assay in isolated otic vesicles shows that Sox2 is bound to the Atoh1 enhancer in vivo. However, besides activating Atoh1, Sox2 also promotes the expression of Atoh1 negative regulators and the temporal profile of Atoh1 activation by Sox2 is transient suggesting that Sox2 triggers an incoherent feed-forward loop. These results provide a mechanism for the prosensory function of Sox2 in the inner ear. We suggest that sensory competence is established early in otic development through the activation of Atoh1 by Sox2, however, hair cell differentiation is prevented until later stages by the parallel activation of negative regulators of Atoh1 function

Modeling the structure of RNA molecules with small-angle X-ray scattering data

We propose a novel fragment assembly method for low-resolution modeling of RNA and show how it may be used along with small-angle X-ray solution scattering (SAXS) data to model low-resolution structures of particles having as many as 12 independent secondary structure elements. We assessed this model-building procedure by using both artificial data on a previously proposed benchmark and publicly available data. With the artificial data, SAXS-guided models show better similarity to native structures than ROSETTA decoys. The publicly available data showed that SAXS-guided models can be used to reinterpret RNA structures previously deposited in the Protein Data Bank. Our approach allows for fast and efficient building of de novo models of RNA using approximate secondary structures that can be readily obtained from existing bioinformatic approaches. We also offer a rigorous assessment of the resolving power of SAXS in the case of small RNA structures, along with a small multimetric benchmark of the proposed method

Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos

hybridization. hybridization (WISH).Here we describe a modification to the WISH protocol that is essential to prevent DNA cross-hybridization and to specifically detect transgene mRNA transcripts in electroporated embryos. Our optimized WISH procedure can be applied not only to electroporated chick embryos but also to other embryos or adult tissues that have been transfected with large amounts of reporter- or expression construct DNA