Differentiation of mesenchymal stem cells into hepatocytes

Acute and chronic liver diseases are common in Kazakhstan and other countries. These diseases are known to cause significant disability and death. In many cases, liver transplantation is the last resort for patients with end stage liver disease, but it is an extremely expensive procedure and is associated with many risks. The most important among them is an immune rejection. Autologous cell transplantation is a potential therapeutic approach for liver regeneration and could become an alternative to organ transplantation. In this regard, mesenchymal stem cells (MSCs) are a very attractive source for differentiation into hepatocytes. These cells can be isolated from bone marrow and adipose tissue of the patient and exponentially expanded in vitro. Transplantation of hepatocytes differentiated from MSCs could become a new promising approach in treatment of the patients with chronic liver conditions

Isolation of primary human hepatocytes from cirrhotic liver

Chronic degenerative liver diseases are among most complex social, clinical and epidemiological health problems worldwide. This is due to the steady increase in the incidence and mortality of patients with this pathology. Orthotopic liver transplantation is the only way to save the lives of patients with decompensated diffuse and focal lesions of the liver. One-year survival after liver transplantation reaches 60-80%, but more than half of the patients on the waiting list do not survive until operation. In this regard, hepatocyte transplantation could be an option for the patients who are on the waiting list for organ transplantation

Differentiation of mesenchymal stem cells into hepatocytes

Acute and chronic liver diseases are common in Kazakhstan and other countries. These diseases are known to cause significant disability and death. In many cases, liver transplantation is the last resort for patients with end stage liver disease, but it is an extremely expensive procedure and is associated with many risks. The most important among them is an immune rejection. Autologous cell transplantation is a potential therapeutic approach for liver regeneration and could become an alternative to organ transplantation. In this regard, mesenchymal stem cells (MSCs) are a very attractive source for differentiation into hepatocytes. These cells can be isolated from bone marrow and adipose tissue of the patient and exponentially expanded in vitro. Transplantation of hepatocytes differentiated from MSCs could become a new promising approach in treatment of the patients with chronic liver conditions

Isolation of primary human hepatocytes from cirrhotic liver

Chronic degenerative liver diseases are among most complex social, clinical and epidemiological health problems worldwide. This is due to the steady increase in the incidence and mortality of patients with this pathology. Orthotopic liver transplantation is the only way to save the lives of patients with decompensated diffuse and focal lesions of the liver. One-year survival after liver transplantation reaches 60-80%, but more than half of the patients on the waiting list do not survive until operation. In this regard, hepatocyte transplantation could be an option for the patients who are on the waiting list for organ transplantation

International Consensus Statement on Rhinology and Allergy: Rhinosinusitis

Background: The 5 years since the publication of the first International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICAR‐RS) has witnessed foundational progress in our understanding and treatment of rhinologic disease. These advances are reflected within the more than 40 new topics covered within the ICAR‐RS‐2021 as well as updates to the original 140 topics. This executive summary consolidates the evidence‐based findings of the document. Methods: ICAR‐RS presents over 180 topics in the forms of evidence‐based reviews with recommendations (EBRRs), evidence‐based reviews, and literature reviews. The highest grade structured recommendations of the EBRR sections are summarized in this executive summary. Results: ICAR‐RS‐2021 covers 22 topics regarding the medical management of RS, which are grade A/B and are presented in the executive summary. Additionally, 4 topics regarding the surgical management of RS are grade A/B and are presented in the executive summary. Finally, a comprehensive evidence‐based management algorithm is provided. Conclusion: This ICAR‐RS‐2021 executive summary provides a compilation of the evidence‐based recommendations for medical and surgical treatment of the most common forms of RS

Agrotechnological methods of plant feeders applying for spring wheat agrocenoses - North-Eastern Kazakhstan varieties

To improve plant resistance to pests, we analysed the impact of various agronomic practices on the number and species diversity of pests in the crops of spring wheat varieties of foreign and Kazakhstan breeding in North-Eastern Kazakhstan. The intensive development of agriculture, resulting in the new technological flow processes of wheat growing, the sowing of foreign varieties not previously cultivated under local conditions, and climate change contribute to the formation of new food chains in agrocenoses. These new food chains require the monitoring of plant-feeding species with the help of ecological approaches and techniques. Efficient protection of crops with plant feeders requires constant updating on the phytosanitary in agrocenoses. Information on phytosanitary monitoring previously carried out in the region is not available, so it became necessary to collect data and analyse the number and species composition of wheat pests, considering new foreign varieties and cultivation technology practices. The research was carried out in 2021 in typical agricultural organisations of the North-Eastern regions of Kazakhstan with different preceding crops. The vegetation period was characterised by high atmospheric temperatures and a lack of moisture in the soil in spring and summer, contributing to decreasing of plant turgor and damage resistance. Early sowing of the ‘Triso’ wheat variety was affected by high temperatures and lack of soil moisture in the initial stage of development, which delayed its growth and made it more susceptible to pest damage. The other varieties were sown in optimal dates recommended by regional scientific institutions