Growth Factors Regulate Expression of Mineral Associated Genes in Cementoblasts

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141778/1/jper1591.pd

Leucine‐Rich Amelogenin Peptide: A Candidate Signaling Molecule During Cementogenesis

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141564/1/jper1126.pd

Future Prospects for Periodontal Bioengineering Using Growth Factors

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142015/1/cap0088.pd

Bone formation at recombinant human bone morphogenetic protein-2-coated titanium implants in the posterior mandible (Type II bone) in dogs

BACKGROUND: Conventional oral/maxillofacial implants reach osseointegration over several months during which the titanium fixtures interact with alveolar bone. The objective of this study was to determine if adsorbing recombinant human bone morphogenetic protein-2 (rhBMP-2) onto a titanium porous oxide (TPO) implant surface might enhance or accelerate local bone formation and support osseointegration in a large animal oral/maxillofacial orthotopic model. MATERIAL AND METHODS: Endosseous implants with a TPO surface were installed into the edentulated posterior mandible in eight adult Hound Labrador mongrel dogs. The implant surface had been adsorbed with rhBMP-2 at 0.2 or 4.0 mg/ml. TPO implants without rhBMP-2 served as control. Treatments were randomized between jaw quadrants. Mucosal flaps were advanced and sutured leaving the implants submerged. Clinical and radiographic evaluations were made immediately post-surgery, at day 10 (suture removal), and week 4 and 8 post-surgery. The animals received fluorescent bone markers at week 3, 4, and at week 8 post-surgery, when they were euthanized for histologic analysis. RESULTS: TPO implants coated with rhBMP-2 exhibited dose-dependent bone remodelling including immediate resorption and formation of implant adjacent bone, and early establishment of clinically relevant osseointegration. The resulting bone-implant contact, although clinically respectable, appeared significantly lower for rhBMP-2-coated implants compared with the control [rhBMP-2 (0.2 mg/ml) 43.3+/-10.8%versus 71.7+/-7.8%, p<0.02; rhBMP-2 (4.0 mg/ml) 35.4+/-10.6%versus 68.2+/-11.0%, p<0.03]. CONCLUSIONS: rhBMP-2 adsorbed onto TPO implant surfaces initiates dose-dependent peri-implant bone re-modelling resulting in the formation of normal, physiologic bone and clinically relevant osseointegration within 8 weeks.This study was supported by a grant from Wyeth Research, Cambridge, MA, USA, and Nobel Biocare AB, Go¨teborg, Sweden. Jan Hall is an employee of Nobel Biocare AB. Rachel G. Sorensen was an employee of Wyeth Research at the time of study. John Wozney is an employee of Wyeth Research. Ulf M. E. Wikesjo¨ was an employee of Wyeth Research, and presently serves as consultant to Wyeth Research and Nobel Biocare AB

The Effect of Suturing Protocols on Coronally Advanced Flap Root-Coverage Outcomes: A Meta-Analysis

The purpose of this systematic review and meta-analysis was to investigate whether suturing protocols (suture removal timing and/or type of suture material) influence root coverage outcomes in recession defects treated with a coronally advanced flap (CAF) procedure. Databases (MEDLINE, EMBASE) were searched for randomized clinical trials (RCTs) that assessed single-tooth, Miller Class I/II recession defects, surgically treated by CAF. Mixed-effects linear regression analysis evaluated differences on complete root coverage (CRC) between RCTs with early (<10 days postoperatively) and late (≥10 days) suture removal, as well as between RCTs using absorbable and non-absorbable sutures removed ≥10 days postoperatively. Seventeen RCTs were eligible for inclusion. Overall, data from 325 single gingival recession defects revealed a statistically significant superior proportion of sites exhibiting CRC when sutures were removed ≥10 days postoperatively compared to those in which sutures were removed <10 days (P = 0.03). Conversely, there were no significant differences in CRC outcomes between absorbable and non-absorbable sutures when they were removed ≥10 days after surgery (P = 1.00). The majority of included RCTs (59%) reported use of non-absorbable suture materials. Within the limitations of the available data, it can be concluded that early suture removal (<10 days) can negatively influence root-coverage outcomes in single-tooth defects treated by a CAF procedure. There is a strong need for studies designed specifically to investigate suturing protocols in root-coverage procedures