102 research outputs found

    Morphocultural and molecular characterization of Colletotrichum gloeosporioides isolates pathogenic to papaya

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    Vinte e nove culturas monospóricas de Colletotrichum, isoladas de frutos e pecíolos de mamoeiro (Carica papaya), foram caracterizadas quanto à morfologia dos conídios e apressórios, coloração e crescimento das colônias, sensibilidade ao benomyl, presença de setas e do teleomorfo, PCR com primers taxon-específicos e análise de PCR-RFLP da região ITS. Os 29 isolados foram identificados como C. gloeosporioides com base na morfologia dos conídios e apressórios, tendo a maioria dos isolados conídios cilíndricos e/ou obclavados e apressórios lobados ou fracamente lobados, em contraste com C. acutatum, isolado de morango (Fragaria x ananassa), que apresentou conídios fusiformes e apressórios circulares e lisos. Presença de setas e do teleomorfo, cor de colônia, sensibilidade ao benomyl e velocidade de crescimento variaram conforme o isolado e sofreram influência do meio de cultura usado. Todos os isolados de mamão e quatro de outras hospedeiras, manga (Mangifera indica), morango e maçã (Malus domestica), foram patogênicos a frutos de mamão cv. Sunrise Solo, mas com variabilidade em agressividade. PCR com o primer específico para C. gloeosporioides, CgInt, confirmou a identidade de apenas quatro isolados de mamão e dois isolados apresentaram reação positiva com o primer CaInt2, específico para C. acutatum. A maioria dos isolados de mamão (23) não reagiu com nenhum dos primers. Por outro lado, a análise de restrição da região ITS do rDNA, com RsaI, gerou perfis distintos entre C. gloeosporioides e C. acutatum e mostrou uniformidade entre os isolados de mamão. _________________________________________________________________________________ ABSTRACTTwenty-nine monoconidial cultures of Colletotrichum isolated from papaya (Carica papaya) petioles and fruits were characterized by conidial and appressoria morphology, colony color, growth rate, sensitivity to benomyl, presence of setae, presence of the teleomorph, PCR with taxon-specific primers and analysis of PCR-RFLP of the ITS region. The 29 isolates from papaya were identified as C. gloeosporioides, based mainly on conidial and appressoria morphology, with most isolates producing cylindrical and/or obclavate conidia and entirely or weakly lobed appressoria, in contrast with the strawberry (Fragaria x ananassa) isolate of C. acutatum, which produced fusiform conidia and circular appressoria with entire edges. Presence of setae, teleomorphic stage, colony color, sensitivity to benomyl and growth rate were variable among isolates and influenced by the culture medium. All papaya isolates and four isolates (C. gloeosporioides and C. acutatum) from other hosts, mango (Mangifera indica), strawberry and apple (Malus domestica), were pathogenic to papaya fruits cv. Sunrise Solo, producing similar symptoms, but with variability in aggressiveness. PCR with C. gloeosporioidesspecific primer, CgInt, confirmed the identity of four papaya isolates. Two other isolates reacted with C. acutatum-specific primer, CaInt2. The majority of papaya isolates (23), however, did not react with any of the primers tested. In contrast, RFLP analysis of the amplified ITS region with RsaI, generated distinct patterns that could differentiate between the two species, C. gloeosporioides and C. acutatum, and showed uniformity among papaya isolates

    Bland-White-Garland Syndrome in an Elderly Woman

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    マンガに描かれる女性の体形と日本人若年女性のボディイメージ

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    日本のマンガの持つ内容の広さ、豊かさ、洗練性および画像表現の自由度は、読む者のボディイメージに強い影響を与える可能性がある。マンガにおける若年女性の体形の描かれ方に関して、ジャンル別(男性向け一女性向け、成人向け-子供向け)の体形の特徴、物語上の役割や人物の性格が体形に及ぼす影響、作者の性別と作品のジャンルの関係について検討した。描かれた身体像は、現代日本の若年女性の標準体形と比較して、若年女子短期大学生らの視点から評価した。男性向けのマンガではやや細いウェストと大きなヒップを持った若年女性が描かれているのに対して、女性向けのマンガではウェスト・ヒップ共に細い若年女性が描かれていた。作品中の若年女性の役割や性格と体形の関係では、女性誌において女性性や母性がいずれも細い身体像と結びつき、また依存性が太いウェストと結びついていた。男性向けのマンガを男性が画いている一方、女性向けのマンガは女性が画いていた。これらの結果から、マンガにおいては非現実的な「やせ理想像」が、女性読者を対象として、女性マンガ家により作られていることが示された
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