9 research outputs found
Production index: A new index to evaluate EPSs as surfactants and bioemulsifiers applied to Halomonas variabilis strain W10 for hydrocarbon bioremediation
This study was partially supported by the Repsol Technology Center
(CTR) Spain and the University of Granada Environmental Research
Group (RNM 270). Additional partial support was provided by the
Spanish Agency of International Cooperation (A.E.C.I.). We also wish to
thank Michael O'Shea for proofreading the paper.Peer Reviewe
Treatment of diesel-polluted clay soil employing combined biostimulation in microcosms
The efficiency of inorganic fertilizers as stimulating agents for the
bioremediation of oil-polluted environments can be increased with the
addition of selected biostimulating compounds. In this study, the
efficacy of different biostimulation treatments in the remediation of
diesel-polluted soil in purpose-built microcosms has been evaluated.
The treatments involved combinations of inorganic fertilizer with (a)
Ivey surfactant, (b) Biorem organic fertilizer and (c) ethanol.
Microbial activity was evaluated by monitoring the growth of
heterotrophic and degrading bacteria and their dehydrogenase activity
and carbon dioxide production. Hydrocarbon degradation was monitored by
gas chromatography/mass spectrometry. The results showed that all
treatments enhanced microbial activity in comparison with natural
attenuation and also that the combined treatments generally enhanced
hydrocarbon biodegradation in comparison to both natural attenuation
and the single inorganic fertilizer treatment. The inorganic fertilizer
plus Ivey® surfactant was the most efficient treatment in terms of
Total Petroleum Hydrocarbon and light and heavy n-alkanes, showing an
index of degradation of 1.4 and 1.3, respectively. Furthermore,
biodegradation of heavy and branched n-alkanes was higher in microcosms
treated with inorganic fertilizer plus ethanol (Index of degradation
values of 1.6 and 1.5, respectively) indicating that combined
treatments can be very effective in restoration of contaminated soil
TGF beta-mediated MMP13 secretion drives myoepithelial cell dependent breast cancer progression
Ductal carcinoma in situ (DCIS) is a non-obligate precursor of invasive breast cancer. Virtually all women with DCIS are treated, despite evidence suggesting up to half would remain with stable, non-threatening, disease. Overtreatment thus presents a pressing issue in DCIS management. To understand the role of the normally tumour suppressive myoepithelial cell in disease progression we present a 3D in vitro model incorporating both luminal and myoepithelial cells in physiomimetic conditions. We demonstrate that DCIS-associated myoepithelial cells promote striking myoepithelial-led invasion of luminal cells, mediated by the collagenase MMP13 through a non-canonical TGFβ - EP300 pathway. In vivo, MMP13 expression is associated with stromal invasion in a murine model of DCIS progression and is elevated in myoepithelial cells of clinical high-grade DCIS cases. Our data identify a key role for myoepithelial-derived MMP13 in facilitating DCIS progression and point the way towards a robust marker for risk stratification in DCIS patients