Human HT-29 colon carcinoma cells contain mucarinic M3_3 receptors coupled to phosphoinositide metabolism

Five different musearlnie receptor subtypes ean be distinguished by the differenees in their amino aeid sequence, the eoupled signal transduetion system, pharmaeologieal binding properties and aetivation of ionie fluxes. The present study served to eharaeterize the binding profile of musearlnie receptors in human eolon eareinoma eells (HT-29) using seleetive musearlnie antagonists. The affinities of the compounds were eompared with their poteney to inhibit cholinergieally-aetivated phosphoinositide metabolism. Pirenzepine displaced [$^3$H]N-methyl-scopolamine binding and inhibited inositolphosphate (IP) release with potencies typieal of those of non-M$_1$ receptors. The M$_3$ subtype-selective antagonists sila-hexocyelium and hexahydro-sila-difenidol bad high affinity to the musearlnie reeeptors in HT-29 cells (K0 = 3.1 nM and 27 nM, respectively) and inhibited IP release at nanomolar concentrations. The M$_2$ receptor antagonists, AF-DX 116 and methoctramine, had low antimusearinic poteneies. Our results demonstrate that HT-29 human colon earcinoma cells contain an apparently pure population of M$_3$ receptors. These cells could serve as a model system for further investigations coneerning regulatory and signal transduction mechanisms associated with glandular muscarinic M$_3$ receptors

Syntbesis and Properries of the Selective Antimuscarinic Agent Cyclohexylphenyl(3-piperidinopropyl)silanol

Die Synthese des selektiven Antimuskarinikums Cyclohexylpheny\{3-piperidinopropyl)sila· nol (1 b) wird beschrieben. 1 b wurde - ausgehend von (3·Chlorpropyl)trimethoxysilan - durch eine vierstufige Reaktionsfolge erhalten und als Hydrochlorid 2b mit einer Gesamtausbeute von etwa 45°/o isoliert. - 1 b ist aufgrund seiner großen pharmakologischen Se· lektivität zu einer Standardsubstanz in der experimentellen Pharmakologie bei der Differenzierung von Muskarinrezeptoren geworden.The synthesis of thc selective antimuscarinic agent cyclohexylphenyl(3-piperidinopropyl)silanol (1 b) is described. Starting with (3-chloropropyl)trimethoxysilane, I b was obtained by four reaction steps and isolated as hydrochloride 2b with a total yield of about 45°/o. - Because of its high pharmacological selectivity 1 b has become a reference drug in experimental pharmacology for the differentiation of muscarinic rcceptors

The Magnetic Casimir Effect

The Casimir effect results from alterations of the zero-point electromagnetic energy introduced by boundary-conditions. For ferromagnetic layers separated by vacuum (or a dielectric) such boundary-conditions are influenced by the magneto-optical Kerr effect. We will show that this gives rise to a long-range magnetic interaction and discuss the effect for two different configurations (magnetization parallel and perpendicular to the layers). Analytical expressions are derived for two models and compared to numerical calculations. Numerical calculations of the effect for Fe are also presented and the possibility of an experimental observation of the Casimir magnetic interaction is discussed

Temperature dependence of the Casimir effect between metallic mirrors

We calculate the Casimir force and free energy for plane metallic mirrors at non-zero temperature. Numerical evaluations are given with temperature and conductivity effects treated simultaneously. The results are compared with the approximation where both effects are treated independently and the corrections simply multiplied. The deviation between the exact and approximated results takes the form of a temperature dependent function for which an analytical expression is given. The knowledge of this function allows simple and accurate estimations at the % level.Comment: 8 pages, 4 figures, uses RevTe

Dynamical Casimir effect at finite temperature

Thermal effects on the creation of particles under the influence of time-dependent boundary conditions are investigated. The dominant temperature correction to the energy radiated by a moving mirror is derived by means of response theory. For a resonantly vibrating cavity the thermal effect on the number of created photons is obtained non-perturbatively. Finite temperatures can enhance the pure vacuum effect by several orders of magnitude. The relevance of finite temperature effects for the experimental verification of the dynamical Casimir effect is addressed.Comment: 9 LaTex page

The Casimir force and the quantum theory of lossy optical cavities

We present a new derivation of the Casimir force between two parallel plane mirrors at zero temperature. The two mirrors and the cavity they enclose are treated as quantum optical networks. They are in general lossy and characterized by frequency dependent reflection amplitudes. The additional fluctuations accompanying losses are deduced from expressions of the optical theorem. A general proof is given for the theorem relating the spectral density inside the cavity to the reflection amplitudes seen by the inner fields. This density determines the vacuum radiation pressure and, therefore, the Casimir force. The force is obtained as an integral over the real frequencies, including the contribution of evanescent waves besides that of ordinary waves, and, then, as an integral over imaginary frequencies. The demonstration relies only on general properties obeyed by real mirrors which also enforce general constraints for the variation of the Casimir force.Comment: 18 pages, 6 figures, minor amendment

The Casimir Problem of Spherical Dielectrics: Numerical Evaluation for General Permittivities

The Casimir mutual free energy F for a system of two dielectric concentric nonmagnetic spherical bodies is calculated, at arbitrary temperatures. The present paper is a continuation of an earlier investigation [Phys. Rev. E {\bf 63}, 051101 (2001)], in which F was evaluated in full only for the case of ideal metals (refractive index n=infinity). Here, analogous results are presented for dielectrics, for some chosen values of n. Our basic calculational method stems from quantum statistical mechanics. The Debye expansions for the Riccati-Bessel functions when carried out to a high order are found to be very useful in practice (thereby overflow/underflow problems are easily avoided), and also to give accurate results even for the lowest values of l down to l=1. Another virtue of the Debye expansions is that the limiting case of metals becomes quite amenable to an analytical treatment in spherical geometry. We first discuss the zero-frequency TE mode problem from a mathematical viewpoint and then, as a physical input, invoke the actual dispersion relations. The result of our analysis, based upon the adoption of the Drude dispersion relation at low frequencies, is that the zero-frequency TE mode does not contribute for a real metal. Accordingly, F turns out in this case to be only one half of the conventional value at high temperatures. The applicability of the Drude model in this context has however been questioned recently, and we do not aim at a complete discussion of this issue here. Existing experiments are low-temperature experiments, and are so far not accurate enough to distinguish between the different predictions. We also calculate explicitly the contribution from the zero-frequency mode for a dielectric. For a dielectric, this zero-frequency problem is absent.Comment: 23 pages, LaTeX, 7 ps figures; expanded discussion, especially in Sec. 5. To appear in Phys. Rev.

Resonant photon creation in a three dimensional oscillating cavity

We analyze the problem of photon creation inside a perfectly conducting, rectangular, three dimensional cavity with one oscillating wall. For some particular values of the frequency of the oscillations the system is resonant. We solve the field equation using multiple scale analysis and show that the total number of photons inside the cavity grows exponentially in time. This is also the case for slightly off-resonance situations. Although the spectrum of a cavity is in general non equidistant, we show that the modes of the electromagnetic field can be coupled, and that the rate of photon creation strongly depends on this coupling. We also analyze the thermal enhancement of the photon creation.Comment: 13 pages. New section on off-resonance motion is included. To appear in Physical Review