5 research outputs found
Suppression of different classes of somatic mutations in Arabidopsis by vir gene-expressing Agrobacterium strains
10.1186/s12870-015-0595-1BMC Plant Biology15121
RNA Interference-Mediated Inhibition of ESCRT in Mammalian Cells
Specific depletion of proteins from cultured cells using RNA interference (RNAi) has
been a useful technique in assessing protein function for many years. RNAi allows
the degradation of specific, targeted mRNA, allowing the effects of protein depletion
on cellular processes to be examined. Here, I present a protocol for the depletion of
proteins from cultured HeLa cells, and list specific reagents and considerations for
targeting the endosomal sorting complexes required for transport (ESCRT)
The long noncoding RNA LUCAT1 promotes colorectal cancer cell proliferation by antagonizing Nucleolin to regulate MYC expression
RNA Therapeutics: How Far Have We Gone?
In recent years, the RNA molecule became one of the most promising targets for therapeutic intervention. Currently, a large number of RNA-based therapeutics are being investigated both at the basic research level and in late-stage clinical trials. Some of them are even already approved for treatment. RNA-based approaches can act at pre-mRNA level (by splicing modulation/correction using antisense oligonucleotides or U1snRNA vectors), at mRNA level (inhibiting gene expression by siRNAs and antisense oligonucleotides) or at DNA level (by editing mutated sequences through the use of CRISPR/Cas). Other RNA approaches include the delivery of in vitro transcribed (IVT) mRNA or the use of oligonucleotides aptamers. Here we review these approaches and their translation into clinics trying to give a brief overview also on the difficulties to its application as well as the research that is being done to overcome them.info:eu-repo/semantics/publishedVersio