Immunophenotypic analysis of erythroid dysplasia in myelodysplastic syndromes. A report from the IMDSFlow working group

Contains fulltext : 189908.pdf (publisher's version ) (Open Access

Modellierung von Bodenprozessen in Agrarlandschaften zur Untersuchung der Auswirkungen moeglicher Klimaveraenderungen Abschlussbericht

The CANDY model meets the requirements of a long term soil model with sufficient representation of short term variations. In order to describe processes of C and N cycle together with temperature and water dynamics in rural areas, CANDY was supplemented by submodels for the variation of physical soil properties and by the integration of a weather generator. Model validation by field tests gave satisfactory results. Preliminary results obtained with integrated weather generator on the basis of results from global climatic models point to a decrease in groundwater production, an increase in microbial activity and consequently a decrease in soil organic matter. (WEN)Available from TIB Hannover: RR 6252(1995,3)+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia

Internal tandem duplications within the juxtamembrane region of the FMS-like tyrosine kinase receptor FLT3 (FLT3-ITD) represents one of the most common mutations in patients with acute myeloid leukemia (AML) which results in constitutive aberrant activation, increased proliferation of leukemic progenitors and is associated with an aggressive clinical phenotype. The expression of CD97, an EGF-TM7 receptor, has been linked to invasive behavior in thyroid and colorectal cancer. Here, we have investigated the association of CD97 with FLT3-ITD and its functional consequences in AML. Higher CD97 expression levels have been detected in 208 out of 385 primary AML samples. This was accompanied by a significantly increased bone marrow blast count as well as by mutations in the FLT3 gene. FLT3-ITD expressing cell lines as MV4-11 and MOLM-13 revealed significantly higher CD97 levels than FLT3 wildtype EOL-1, OCI-AML3 and HL-60 cells which were clearly decreased by the tyrosine kinase inhibitors PKC412 and SU5614. CD97 knock down by short hairpin RNA in MV4-11 cells resulted in inhibited trans-well migration towards fetal calf serum (FCS) and lysophosphatidic acid (LPA) being at least in part Rho-A dependent. Moreover, knock down of CD97 led to an altered mechanical phenotype, reduced adhesion to a stromal layer and lower wildtype FLT3 expression. Our results, thus, constitute the first evidence for the functional relevance of CD97 expression in FLT3-ITD AML cells rendering it a potential new theragnostic target