Light Sheet Microscopy for Single Molecule Tracking in Living Tissue

Single molecule observation in cells and tissue allows the analysis of physiological processes with molecular detail, but it still represents a major methodological challenge. Here we introduce a microscopic technique that combines light sheet optical sectioning microscopy and ultra sensitive high-speed imaging. By this approach it is possible to observe single fluorescent biomolecules in solution, living cells and even tissue with an unprecedented speed and signal-to-noise ratio deep within the sample. Thereby we could directly observe and track small and large tracer molecules in aqueous solution. Furthermore, we demonstrated the feasibility to visualize the dynamics of single tracer molecules and native messenger ribonucleoprotein particles (mRNPs) in salivary gland cell nuclei of Chironomus tentans larvae up to 200 µm within the specimen with an excellent signal quality. Thus single molecule light sheet based fluorescence microscopy allows analyzing molecular diffusion and interactions in complex biological systems

Grenzschichten in der Mundhoehle als Verursacher von Dosiserhoehungen bei Anwendung ionisierender Strahlung

Copy held by FIZ Karlsruhe; available from UB/TIB Hannover / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Comparing interface dosimetry with conventional methods and TSEE.

Dose enhancement factors at plane TEM/metal interfaces have experimentally been determined for photon beams. The measurements were performed ionometrically and, for comparison, with solid state dosemeters. Emphasis was given to TSEE allowing for transition-zone dosimetry on a near-microscopic scale. Dose enhancement of as much as 58 directs interest towards radiological and technical applications