3-D neurohistology of transparent tongue in health and injury with optical clearing

Tongue receives extensive innervation to perform taste, sensory, and motor functions. Details of the tongue neuroanatomy and its plasticity in response to injury offer insights to investigate tongue neurophysiology and pathophysiology. However, due to the dispersed nature of the neural network, standard histology cannot provide a global view of the innervation. We prepared transparent mouse tongue by optical clearing to reveal the spatial features of the tongue innervation and its remodeling in injury. Immunostaining of neuronal markers, including PGP9.5 (pan-neuronal marker), calcitonin gene-related peptide (sensory nerves), tyrosine hydroxylase (sympathetic nerves), and vesicular acetylcholine transporter (cholinergic parasympathetic nerves and neuromuscular junctions), was combined with vessel painting and nuclear staining to label the tissue network and architecture. The tongue specimens were immersed in the optical-clearing solution to facilitate photon penetration for 3-dimensiontal (3-D) confocal microscopy. Taking advantage of the transparent tissue, we simultaneously revealed the tongue microstructure and innervation with subcellular-level resolution. 3-D projection of the papillary neurovascular complex and taste bud innervation was used to demonstrate the spatial features of tongue mucosa and the panoramic imaging approach. In the tongue injury induced by 4-nitroquinoline 1-oxide administration in the drinking water, we observed neural tissue remodeling in response to the changes of mucosal and muscular structures. Neural networks and the neuromuscular junctions were both found rearranged at the peri-lesional region, suggesting the nerve-lesion interactions in response to injury. Overall, this new tongue histological approach provides a useful tool for 3-D imaging of neural tissues to better characterize their roles with the mucosal and muscular components in health and disease

The integration of storm water flooding and thermal stress potential in Tainan (Taiwan) and Groningen (Netherlands)

Stormwater flooding and thermal stresses of citizens are two important phenomena for most of the dense urban area. Due to the climate change, these two phenomena will occur more frequently and cause serious problems. Therefore, the sectors for public health and disaster management should be able to assess the vulnerability to stormwater flooding and thermal stress. To achieve this goal, two cities in different climate regions and with different urban context have been selected as the pilot areas, i.eY., Tainan, Taiwan and Groningen, Netherlands. Stormwater flooding and thermal stress maps will be produced for both cities for further comparison. The flooding map indicates vulnerable low lying areas, where the thermal stress map indicates high Physiological Equivalent Temperature (PET) values (thermal comfort) in open areas without shading. The combined map indicates the problem areas of flooding and thermal stress and can be used by urban planners and other stakeholders to improve the living environment

Exploring the effect of AR filter on perceived brand image: The mediating role of realistic experience

Following its extensive application in study on consumers\u27 retail experience, AR (augmented reality) technology has been increasingly applied in social media, notably the AR filter service, which has been embraced by quite a few brands as a novel marketing tool, taking advantage of its entertaining value and dissemination effect to boost brands\u27 perceived image among consumers. However, there have been fewer studies that examine the effectiveness of brand-developed AR filter services in enhancing consumer perceptions of brand image in the context of social media. To bridge the research gap, the study, based on the S-O-R model, looked into the effect of consumers\u27 cognition of AR-filter features on the realism of their experience and thus their perceived brand image. The study was carried out via a questionnaire survey of users of AR filters developed by brand-developed in social media, with its findings having both academic and practical implications for this field

A Survey of Generative Information Retrieval

Generative Retrieval (GR) is an emerging paradigm in information retrieval that leverages generative models to directly map queries to relevant document identifiers (DocIDs) without the need for traditional query processing or document reranking. This survey provides a comprehensive overview of GR, highlighting key developments, indexing and retrieval strategies, and challenges. We discuss various document identifier strategies, including numerical and string-based identifiers, and explore different document representation methods. Our primary contribution lies in outlining future research directions that could profoundly impact the field: improving the quality of query generation, exploring learnable document identifiers, enhancing scalability, and integrating GR with multi-task learning frameworks. By examining state-of-the-art GR techniques and their applications, this survey aims to provide a foundational understanding of GR and inspire further innovations in this transformative approach to information retrieval. We also make the complementary materials such as paper collection publicly available at https://github.com/MiuLab/GenIR-Survey

KINEMATICS ANALYSIS OF THE UPPER EXTREMITY DURING THE TWOHANDED BACKHAND DRIVE VOLLEY FOR FEMALE TENNIS PLAYERS

The purpose of this study was to discuss the motion characteristics of the arms in the two-handed backhand drive volley. Five elite female tennis players participated in this study, their two-handed backhand drive volley strokes were analysed, and all participants are right handed. Motion Analysis System with 10 Eagle Digital inferred high speed cameras at 200Hz were used for this study. The results show a similar elbow and wrist speed strategy in x-axis between two-handed ground stroke and drive volley, our study also found that the rear arm dominates the stroke and mainly provide the topspin that is required for the skill of the drive volley. In order to create better stroke efficiency, the right elbow reached peak velocity first, followed by the right wrist before racket impact with the ball

Explore the effects of social interactions and anxiety symptoms on quality of life in schizophrenia patients

Session presented on: Wednesday, July 24, 2013: Purpose: This study was to explore the effects of social interaction and anxiety symptoms on quality of life in schizophrenia patients. Methods: This is a cross-sectional, correlational study. Convenience sample was conducted in northern medical hospitals (excluding the schizophrenia cases treated in acute ward). Qualified subjects were 143 schizophrenia patients. Instruments included four parts: demographic data, Assessment of Communication and Interaction Skills Chinese version (ACIS-C), Beck Anxiety Inventory and WHOQOL-Bref. Assessment of Communication and Interaction Skills was collected through individual interview or group observation, while the others were collected by self-report. SPSS 17.0 was employed to conduct descriptive statistics on mean, standard deviation, median after the data was collected. As for the inference statistics, it included t-test, Mann-Whitney U test, one way Analysis of Variance, Kruskal-Wallis test, Pearson Correlation and used multiple regression to examine the important predictable variables of quality of life in schizophrenia patients. Results: The research results are as the following: 1. There was a negative correlation between length of illness and social interaction. 2. The mean of Beck Anxiety Inventory was 15.01 (SD = 13.55); the younger the age is, the anxiety symptoms increase, and the longer the illness is, the anxiety symptoms decrease. (3) Anxiety symptoms can negatively affect the quality of life. Conclusion: This research suggests nursing staffs should adopt more effective nursing interventions for anxiety symptoms to improve the quality of life

Synergistic effect of imp/ostA and msbA in hydrophobic drug resistance of Helicobacter pylori

<p>Abstract</p> <p>Background</p> <p>Contamination of endoscopy equipment by <it>Helicobacter pylori </it>(<it>H. pylori</it>) frequently occurs after endoscopic examination of <it>H. pylori</it>-infected patients. In the hospital, manual pre-cleaning and soaking in glutaraldehyde is an important process to disinfect endoscopes. However, this might not be sufficient to remove <it>H. pylori </it>completely, and some glutaraldehyde-resistant bacteria might survive and be passed to the next patient undergoing endoscopic examination through unidentified mechanisms. We identified an Imp/OstA protein associated with glutaraldehyde resistance in a clinical strain, NTUH-C1, from our previous study. To better understand and manage the problem of glutaraldehyde resistance, we further investigated its mechanism.</p> <p>Results</p> <p>The minimal inhibitory concentrations (MICs) of glutaraldehyde andexpression of <it>imp/ostA </it>RNA in 11 clinical isolates from the National Taiwan University Hospital were determined. After glutaraldehyde treatment, RNA expression in the strains with the MICs of 4–10 μg/ml was higher than that in strains with the MICs of 1–3 μg/ml. We examined the full-genome expression of strain NTUH-S1 after glutaraldehyde treatment using a microarray and found that 40 genes were upregulated and 31 genes were downregulated. Among the upregulated genes, <it>imp/ostA </it>and <it>msbA</it>, two putative lipopolysaccharide biogenesis genes, were selected for further characterization. The sensitivity to glutaraldehyde or hydrophobic drugs increased in both of <it>imp/ostA </it>and <it>msbA </it>single mutants. The <it>imp/ostA </it>and <it>msbA </it>double mutant was also hypersensitive to these chemicals. The lipopolysaccharide contents decreased in individual <it>imp/ostA </it>and <it>msbA </it>mutants and dramatically reduced in the <it>imp/ostA </it>and <it>msbA </it>double mutant. Outer membrane permeability assay demonstrated that the <it>imp/ostA </it>and <it>msbA </it>double mutation resulted in the increase of outer membrane permeability. Ethidium bromide accumulation assay demonstrated that MsbA was involved in efflux of hydrophobic drugs.</p> <p>Conclusion</p> <p>The expression levels of <it>imp/ostA </it>and <it>msbA </it>were correlated with glutaraldehyde resistance in clinical isolates after glutaraldehyde treatment. Imp/OstA and MsbA play a synergistic role in hydrophobic drugs resistance and lipopolysaccharide biogenesis in <it>H. pylori</it>.</p