Study of internal structures of 9,10Be and 10B in scattering of 4He from 9Be

A study of inelastic scattering and single-particle transfer reactions was performed by an alpha beam at 63 MeV on a 9$Be target. Angular distributions of the differential cross sections for the 9Be(4He,4He')9Be*, 9Be(4He,3He)10Be and 9Be(4He,t)10B reactions were measured. Experimental angular distributions of the differential cross sections for the ground state and a few low-lying states were analyzed in the framework of the optical model, coupled channels and distorted-wave Born approximation. An analysis of the obtained spectroscopic factors was performed.Comment: 16 pages, 7 figures, 3 tables, regular paper, mispritns are corrected in new versio

Polymorphonuclear myeloid-derived suppressor cells limit antigen crosspresentation by dendritic cells in cancer

DCs are a critical component of immune responses in cancer primarily due to their ability to cross-present tumor-associated antigens. Cross-presentation by DCs in cancer is impaired, which may represent one of the obstacles for the success of cancer immunotherapies. Here, we report that polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) blocked crosspresentation by DCs without affecting direct presentation of antigens by these cells. This effect did not require direct cell-cell contact and was associated with transfer of lipids. Neutrophils (PMN) and PMN-MDSC transferred lipid to DCs equally well; however, PMN did not affect DC cross-presentation. PMN-MDSC generate oxidatively truncated lipids previously shown to be involved in impaired cross-presentation by DCs. Accumulation of oxidized lipids in PMN-MDSC was dependent on myeloperoxidase (MPO). MPO-deficient PMN-MDSC did not affect cross-presentation by DCs. Cross-presentation of tumor-associated antigens in vivo by DCs was improved in MDSC-depleted or tumor-bearing MPO-KO mice. Pharmacological inhibition of MPO in combination with checkpoint blockade reduced tumor progression in different tumor models. These data suggest MPO-driven lipid peroxidation in PMN-MDSC as a possible non–cell autonomous mechanism of inhibition of antigen cross-presentation by DCs and propose MPO as potential therapeutic target to enhance the efficacy of current immunotherapies for patients with cancer

The role of DNA methylation in the disorders of bone metabolism

Osteoporosis is one of multifactorial diseases, it develops from interactions between the genetic component and the environment. However, the universal epigenetic markers of osteoporosis are not yet defined. Finding the risk factors will predict the risk of osteoporosis at the preclinical stage, help define the course and severity of the disease, and develop preventive measures based on them to reduce the risk of fractures. Expanding knowledge in the field of bone biology, especially in the genetics of osteoporosis and osteoimmunology, showed that osteoporosis is a disease that occurs not only due to hormonal or mechanical disorders, but also as a clinically and genetically heterogeneous disease, and there are still unknown pathogenetic links in its structure. Decreases in bone mass and matrix mineralization as well as changes in bone microarchitecture can have different pathogenetic patterns of development and, moreover, there are unknown links of the pathogenesis of osteoporosis. It is possible that DNA methylation is one of these links and a mechanism for epigenetic regulation of gene expression. Evidence exists that this mechanism alongside regulatory miRNAs and post-translational modifications makes a significant contribution to the central processes of bone remodeling; however, the results of various studies vary greatly, and, therefore, there is a need to understand the significance of the accumulated data and to make them consistent. The purpose of this review is to compile and systematize data on the role of DNA methylation in bone metabolism in normal and pathological conditions, in the formation of osteoporosis, and to assess achievements and trends in this field of research and technologies for studying DNA methylation

Association of Toll-Like Cell Receptors TLR2 (p.Arg753GLN) and TLR4 (p.Asp299GLY) Polymorphisms with Indicators of General and Local Immunity in Patients with Atopic Dermatitis

© 2017 Yury A. Tyurin et al.A whole group of polymorphisms of genes involved in the formation of the epidermal barrier, immune responses, and their regulation is important in the formation of atopic phenotype. The purpose of the study is to determine the relationship of polymorphisms of genes of Toll-like receptors TLR2 and TLR4 with clinical and immunological parameters in atopic dermatitis patients in a "case-control" study. Polymorphisms of genes TLR2 (p.Arg753Gln) and TLR4 (Asp299Gly) were detected by PCR. Parameters of the state of innate and adaptive immunity were assessed by the level of local production of sIgA, cytokine profile of blood serum for IL-4, IL-10, and IFN-γ. Biological samples from 50 people with allergic pathology, aged 4.5 to 35 years, and 100 healthy individuals (controls) were analyzed. Observed dysregulation of cytokine production (IL-4, IL-10) in patients with heterozygous polymorphic genotypes probably reflects an imbalance of Th1/Th2/Th17 regulation of immune system response in these individuals

Mirror duality and noncommutative tori

In this paper, we study a mirror duality on a generalized complex torus and a noncommutative complex torus. First, we derive a symplectic version of Riemann condition using mirror duality on ordinary complex tori. Based on this we will find a mirror correspondence on generalized complex tori and generalize the mirror duality on complex tori to the case of noncommutative complex tori.Comment: 22pages, no figure

Cytokine Profile of Patients with Allergic Rhinitis Caused by Pollen, Mite, and Microbial Allergen Sensitization

© 2017 Yury A. Tyurin et al. Allergic rhinitis (AR) is especially prevalent among the population of large cities. Immunologically, the airway epithelium is a region where the population of allergen-presenting cells concentrates. These cells actively express a group of receptors of the innate immune system. A specific cytokine profile is its representation. The study was aimed at evaluating the cytokine profile in patients with seasonal and perennial allergic rhinitis. The cytokine profile of nasal secretion and blood serum of 44 patients with AR was studied. 24 of them had seasonal allergic rhinitis (SAR), and 20 patients suffered from perennial allergic rhinitis (PAR). The patients' age ranged from 4 to 60 years. It was determined in our study that the activation of the GM-CSF production retained in patients with PAR sensitized to mite allergen components (Dermatophagoides pteronyssinus). There was a higher production profile of TNF-α and TSLP in nasal secretion in the patients with perennial allergic rhinitis and additional high sensitization to SEs. Sensitization to mold fungal allergen components significantly increases in patients with seasonal allergic rhinitis. They demonstrated high level of sensitization to the Aspergillus fumigatus component m3. Thus, along with other clinical trials, the study performed would clarify some aspects of molecular pathogenesis of human allergic rhinitis

Characteristics of Antibiotic Resistance Nasopharyngeal Strains of Streptococcus pneumoniae in Children Suffering from Respiratory Pathologies

© 2016, Springer Science+Business Media New York.Data have been obtained on dynamics of antibiotic susceptibility of Streptococcus pneumoniae isolates circulating in preschool children suffering from respiratory pathologies, in the city of Kazan in the years 2009–2015. The high activity of β-lactam antibiotics (from 96 to 90.8 %, depending on the period considered), macrolides (from 83.7 to 93.4 %), and ciprofloxacin (78.9–73.5 %) for the causal treatment of pneumococcal infections. No vancomycin-resistant strains of pneumococci were registered. Comparative analysis of 2009–2011 and 2015 showed a statistically significant increase of the ratio of resistant strains to penicillin (3.5 %), amoxicillin/clavulanate (3.9 %), amoxicillin (5.3 %), clarithromycin (3.6 %), clindamycin (2.9 %), ceftriaxone (5.2 %), and cefixime (2.3 %). According to data on serotyping of isolates, serotypes of S. pneumoniae are covered by the 13-valent conjugate vaccine currently used in children by the national calendar of preventive vaccination

Structure and microhardness of titanium-based coatings formed by multichamber detonation sprayer

A series of titanium-based coatings (50-550 m thick) on an aluminium substrate was deposited via multichamber detonation sprayer with different barrel lengths (300, 400, and 500, all in mm). The titanium-based coatings obtained in these three experiments were examined by optical microscopy, scanning electron microscopy, and X-ray phase analysi