Suited

Jennifer Morla, a contemporary designer, once said, “asking questions generates more ideas.” I am an external processor, I tend to think out loud in order to generate innovative ideas. When I create work, I do not only think about solving the problem, I also think about influencing the visual world around me, because being a designer gives me power. Design helps others to see the world through my vibrant lens. Suited is about the confidence, character, and style that comes with the decision to be bold in both attitude and appearance. I want women who see the magazine to be inspired and believe in the potential they have to encourage and lead those around them. Through the design for this brand, I want to evoke powerful positive emotions. I plan to suggest these emotions through sleek typography and bold photography. I pair both vibrant and subtle colors together to make it easier for the viewer to navigate through the magazine. I use my designs to evoke an emotion by pushing ideas, manipulating line, experimenting with shapes and colors, and observing detail. My love for sleek serif fonts such as Didot aided to the design of the Suited font. I paired it with Barlow which is a condensed sans serif font that compliments the serif. I chose a colorful palette for the interior of the magazine to create hierarchy and aid the direction for the viewer. My work is visually influenced by Herb Lubalin. He would manipulate type in such an Illustrative and expressive way, that it would be both an image and typography. I like to express an idea with fonts rather than with imagery, and he is an advocate for this. I am most inspired by his design of the font “Avant Garde.” Zuzana Licko and her husband created Émigré magazine which is a magazine created by immigrants for immigrants. The magazine became known as a “revolutionary design publication.” The fonts made for this magazine were so popular that the magazine became a type foundry. She changed the contemporary design world around her, which to me is powerful.https://digitalcommons.murraystate.edu/art498/1066/thumbnail.jp

Deciphering the Neuroprotective Potential of Interleukin-1β (IL-1β) using in vitro Murine Models of Oxidative Stress

Interleukin-1β (IL-1β), a key cytokine driving neuroinflammation in the Central Nervous System (CNS), is enhanced in many neurological diseases/disorders, including but not limited to ischemic stroke, Parkinson\u27s disease, Amyotrophic Lateral Sclerosis, Huntington\u27s disease and Alzheimer\u27s disease. The dominant view is that IL-1β contributes to and/or sustains pathophysiological processes. However, other studies demonstrate that IL-1β can play an important role in neural protection and repair. It may do so by modifying astrocyte behavior. Indeed, studies from our laboratory demonstrate that IL-1β increases the synthesis and release of the antioxidant molecule glutathione (GSH) from astrocytes and that IL-1β-treated astrocytes show increased resistance to oxidative stress [He, Jackman et al. 2015], a pathological process that leads to neural damage in the above-mentioned maladies. Given this, the overall goal of this thesis was to study the protective potential of IL-1β against oxidant injury in astrocytes, neurons and astrocyte and neurons in co-culture. We confirmed that IL-1β mediates an increase in extracellular GSH levels in cortical wild-type astrocytic (as shown by our laboratory earlier [He, Jackman et al. 2015]) and mixed cultures but not neuronal culture. IL-1β-mediated GSH enhancement rendered protection to mixed culture against oxidative stress induced by the stressor tert-butyl hydroperoxide (t-BOOH). GSH production and the resultant protection were blocked by the inhibition of GSH transport through Mrp1. Additionally, IL-1β failed to increase GSH or to provide protection against t-BOOH toxicity in chimeric cultures made using il1r1 null mutant astrocytes (lacking a functional IL-1R1 receptor) indicating the necessity of astrocytic signaling for the observed phenomenon. Overall, these findings suggest that under certain conditions IL-1β may be an important stimulus for GSH production and consequent neuroprotection through astrocyte-specific signaling

Theoretical Investigation of Magnetic Properties of 3d and 4f Compounds

3d and 4f complexes are studied by quantum chemical methods to understand their magnetic behavior. For the theoretical description, methods are needed that can take the open shell character of the molecules, several electronic states and spin orbit coupling into account. In this work, ab initio calculations have been performed to understand the magnetic behavior of the transition metal and lanthanide based systems. The investigations were focused on 3d/4f systems with different ligand fields and geometries

Twinkle Bhojwani

Being a young designer, it is sometimes inevitable to not have a fickle mind as I try to grow. I want to create aesthetically pleasing work by pushing my brain to move beyond my first idea in order to develop original approaches to the challenges that arise, and that is why I design. I see poor design that evokes an emotion in me to want to fix it. The way things are designed, whether good or bad, communicates just as boldly as the message or the idea. I use my designs to evoke an emotion by pushing ideas, manipulating line, experimenting with shapes and colors, and observing detail. I have made it more interesting by having a relationship with my design. My work ranges from the use of thin and heavy typography, sleek imagery, and bold, solid colors. My design stems more towards designing business systems, package design, advertisements, and layout designs. All of my work has a lot in common, for example in most of my designs I lean more towards serif fonts, lots of white space, and the use of typography more than imagery. Jennifer Morla, a contemporary designer, once said, “asking questions generates more ideas.” I am definitely an external processor, so I tend to think out loud in order to generate ideas. Design heavily influences contemporary society and is influenced by society. When I create work, I do not only think about solving the problem, I also think about changing the contemporary world around me, because being a designer, gives me power. Design gets me one step closer to others seeing the world through my lens. My work is visually influenced by Herb Lubalin. He would manipulate type in such a creative way, that it would look like an image rather than typography. I like to experiment with fonts more than with imagery, and he is an advocate for this. I am most inspired by his design of the font “Avant Garde.”https://digitalcommons.murraystate.edu/art399/1078/thumbnail.jp

Expression, subcellular localization and functional characterization of RBM5 and RMB10 during the differentiation of C2C12 skeletal myoblasts (skeletal myogenesis)

RNA-binding proteins (RBPs) are a highly regulated, evolutionarily conserved and functionally distinct family of proteins involved in key RNA metabolic processes. The RNA-binding motif protein RBM5 is an anti-proliferative, pro-apoptotic, putative tumor suppressor. A paralogue of RBM5, RBM10, which shares 50% identity with RBM5, functions in development. RBM5 and RBM10 are spliceosomal components involved in alternative splicing. RBM5 and RBM10 are ubiquitously expressed with higher levels in muscle (heart and skeletal) and pancreas. Most of the studies on RBM5 and RBM10 have been focused on cancer cells. Several factors such as 1) abundance in muscle, 2) developmental and temporal regulation, 3) alternative splicing activity and 4) association with functional events related to muscle development led us to hypothesize that both RBM5 and RBM10 are involved in skeletal muscle differentiation. The mechanism of action through which these two RBPs effect differentiation is hypothesized to involve alternative splicing of muscle differentiation-specific mRNAs. RBM5 and RBM10 expression and intracellular distribution was analyzed during muscle differentiation in the C2C12 murine model using qPCR, end-point PCR, immunoblotting and confocal microscopy. Also, RBM5 and RBM10 levels were transiently down-regulated using siRNA either separately and/or together and the associated changes in cell phenotype, expression of myogenic proteins plus a few alternative splicing events were analyzed. We observed a decrease in RBM5 and RBM10 protein expression levels in the differentiated myotubes compared to the myoblasts and myocytes, which indicates a time-dependent potential regulatory role during differentiation. Further, changes in RBM5 and RBM10 protein expression without modulating the levels of mRNA variants suggests posttranscriptional and/or post-translational regulation. Stage-specific differential localization suggests multiple functions related to mRNA biogenesis. RBM5-depleted cells showed a reduction in the total cell number during differentiation, and exhibited a delay in differentiation, fusion and maturation with down-regulated expression of myogenin and myosin heavy chain (MyHC). This implies that RBM5 is necessary to maintain the cell population to execute the myogenic differentiation process in a timely manner. RBM10- depleted cells showed an increase in total cell number immediately after transfection, and exhibited a delay in differentiation with a decrease in inclusion of exon 11 in Dtna mRNA. This indicates that RBM10 is required to maintain the necessary cell population before induction and acts as a splicing regulator during differentiation. RBM5- and RBM10- depleted cells differentiated and matured slowly, and had an increase in Mef2c γ exon inclusion. Therefore, these two RBPs are associated with the alternative splicing of Mef2cγ during differentiation. This is the first study to analyze the expression and the function of these two RBPs in a murine skeletal muscle differentiation model, and has implicated them in myogenesis, paving a way for further characterization. Future studies can investigate the involvement of RBM5 and RBM10 in disease states such as muscular dystrophy and rhabdomyosarcomas, given the known functions of RBPs in tumorigenesis in other cell types.Doctor of Philosophy (PhD) in Biomolecular Science

The Feminist Approach and the Fictional World of Nayantara Sahgal: A Critical Study

Not availabl

Improved User Shopping Journey

Computer-implemented systems and methods provide for an improved user shopping experience via aggregated product data. In one embodiment, in response to a query, a system generates a user interface with multiple sections. A first section may include learning materials and information pertaining to the query to aid a user in making an informed purchase. A second section may include an overview of available products based on price and given features, as well as feature filters to narrow results. A third section may include a comparison tool that allows the user to directly compare products with the same brand, as well as compare individual products across multiple brands