Severe renal failure and hyperammonemia in a newborn with propionic acidemia: effects of treatment on the clinical course

Neonatal-onset propionic acidemia (PA), the most common form, is characterized by poor feeding, vomiting, and somnolence in the first days of life in a previously healthy infant, followed by lethargy, seizures, and can progress to coma if not identified and treated appropriately. It is frequently accompanied by metabolic acidosis with anion gap, ketonuria, hypoglycemia, hyperammonemia, and cytopenias. PA is caused by deficiency of propionyl-CoA carboxylase (PCC), the enzyme that catalyzes the conversion of propionyl-CoA to methylmalonyl-CoA. Herein, we report a case of 3-day-old neonate with PA presented with acute renal failure and metabolic acidosis was effectively treated by peritoneal dialysis and conventional methods

Clinical characteristics and molecular genetic analysis of 22 patients with neonatal diabetes from the south-eastern region of Turkey: predominance of non-KATP channel mutations

Background: neonatal diabetes mellitus (NDM), is a rare form of monogenic diabetes, and usually presents in the first 6 months of life. We aimed to describe the clinical characteristics and molecular genetics of a large Turkish cohort of NDM patients from a single centre and estimate an annual incidence rate of NDM in South-Eastern Anatolian region of Turkey. Design and Methods: NDM patients presenting to Diyarbakir Children State Hospital between 2010 and 2013, and patients under follow up with presumed type 1 diabetes mellitus, with onset before 6 months of age were recruited. Molecular genetic analysis performed. Results: twenty-two patients(59% males) were diagnosed with NDM(TNDM-5;PNDM-17). Molecular genetic analysis identified a mutation in 20(95%) patients who a mutation analysis was undertaken. In TNDM patients, the genetic cause included chromosome 6q24 abnormalities(n=3), ABCC8(n=1) and homozygous INS(n=1). In PNDM patients, homozygous GCK(n=6), EIF2AK3(n=3), PTF1A(n=3), and INS(n=1) and heterozygous KCNJ11(n=2) mutations were identified. Pancreatic exocrine dysfunction was observed in patients with mutations in the distal PTF1A enhancer. Both patients with a KCNJ11 mutation responded to oral sulfonylurea. A variable phenotype was associated with the homozygous c.-331C>A INS mutation, which was identified in both a PNDM and TNDM patient. The annual incidence of PNDM in South-East Anatolian region of Turkey was 1 in 48,000 live births. Conclusions: homozygous mutations in GCK, EIF2AK3 and the distal enhancer region of PTF1A were the commonest causes of NDM in our cohort. The high rate of detection a mutation likely reflects the contribution of new genetic techniques (targeted next generation sequencing) and increased consanguinity within our cohor