Role of Hepcidin and Pro-Inflammatory Cytokines in Chronic Heart Failure in Combination with Anemia

Objectives: To study the effect of hepcidin on the course of chronic heart failure with anemia. Methods: 115 patients with CHF caused by ischemic heart disease (IHD) were chosen. All of them were treated in in-patient conditions and were followed in out-patient conditions. The patients were divided into two main and one control groups. Main group A consisted of 40 CHF patients with iron deficiency anemia. Main group B consisted of 35 CHF patients with anemia of chronic disease. 40 CHF patients without anemia were chosen for the control group. To patients of the main groups 200mg of Iron (III) hydroxide (Venofer®) was administered intravenously once in two days, along with standard CHF treatment. Results: Our study found reliable increase of serum levels hepcidin in CHF patients with anemia of chronic disease compared to CHF patients with iron deficiency anemia (45,4%) and control group (33%) (P\u3c0,001). This, in turn, shows that hepcidin is an important indicator in differential diagnosis between anemia of chronic disease and iron deficiency anemia. Statistical analysis of pro-inflammatory cytokines and hepcidin levels was done in order to find any associations in patients of our study. Following was found: in group B weak positive correlation between hepcidin and IL-1 (r=0,24 Р\u3c0,05), strong positive correlation between hepcidin and IL-6 (r=0,52, Р\u3c0,001), and moderate positive correlation between hepcidin and TNF-α (r=0,37, Р\u3c0,05). Conclusion: In CHF patients with anemia of chronic disease (Group B) hepcidin levels were reliably higher than normal ranges and correlation between pro-inflammatory cytokines and hepcidin during deterioration of the disease

Screening of SNPs at 18 positional candidate genes, located within the GD-1 locus on chromosome 14q23-q32, for susceptibility to Graves' disease: A TDT study

Graves' disease (GD) is a complex autoimmune thyroid disorder with a strong genetic component. Genome-wide screens resolved several susceptibility loci that contribute to the development of GD. One of the susceptibility loci (GD-1 locus) was mapped on chromosome 14q31. However, a susceptibility gene located within the GD-1 locus remains undefined. Here we screen eighteen single nucleotide polymorphisms (SNPs), each is situated at a corresponding positional candidate gene, located within the GD-1 susceptibility locus on chromosome 14q23-q32, for predisposition to GD using the transmission disequilibrium test in 126 simplex Russian families affected with GD. Among SNPs tested, a significant preferential transmission of the Ala allele (41 transmissions vs. 17 nontransmissions, corrected P = 0.031) of the Thr92Ala SNP within the DIO2 gene, encoding type II iodothyronine deiodinase, from parents to affected children was found in a Russian family data set. The Thr92Ala SNP of the DIO2 gene and the D727E substitution of the thyrotropin receptor (TSHR) gene have been found to be in pair-wise linkage disequilibrium. The A92/E727 haplotype showed significant preferential transmission from parents to affected sibling (17 transmissions vs. 8 nontransmissions, P = 0.039) in simplex families. This suggests that the Thr92Ala variant of the DIO2 gene is associated or may be in linkage disequilibrium with a functional DIO2 polymorphism which involves in the development of GD in a Russian population

Polymorphism of Angiotensinogen T174M Gene and Cardiovascular Diseases in the Moscow Population

The groups of patients with myocardial infarction (MI) and hypertrophy of the left ventricle (HLV) (n = 45 and n = 53, respectively) and a sample of healthy individuals from the Moscow population (n = 60) were examined for T174M polymorphism of ACT gene (replacement of methionine for threonine at position 174 of the correspondent amino acid sequence). In MI patients the content of 7T genotypes and 7 allele was significantly lower than in the control group (57.8% against 80% and 67.9 against 89.2%, respectively), whereas the proportion of M allele and TM hétérozygotes was increased (32.1 against 10.8% and 37.8 against 18.3%, respectively). In patients with HLV, the proportion of 7T genotype (64.2%) and T allele (77.4%) was also lower than in the control group, whereas the frequency of M allele was increased (22.6%). Our results suggest that the T174M polymorphism of ACT gene is associated with MI and HLV in the Moscow population

Association between polymorphism T174M of the angiotensinogen gene and arterial hypertension in muscovites

[No abstract available

Polymorphism of Angiotensinogen II T174M Gene and Cardiovascular Diseases in the Moscow Population

Groups of patients with myocardial infarction (MI) and left ventricular hypertrophy (LVH) (n = 45 and n = 53, respectively) and a sample of healthy individuals from the Moscow population (n = 60) were examined for T174M polymorphism of AGT gene (replacement of methionine for threonine at position 174 of the correspondent amino acid sequence). In MI patients, the content of TT genotypes and T allele was significantly lower than in the control group (57.8 vs. 80% and 67.9 vs. 89.2%, respectively), whereas the proportion of M allele and TM heterozygotes was increased (32.1 vs. 10.8% and 37.8 vs. 18.3%, respectively). In patients with LVH, the proportion of TT genotype (64.2%) and T allele (77.4%) was also lower than in the control group, whereas the frequency of M allele was increased (22.6%). Our results suggest that the T174M polymorphism of AGT gene is associated with MI and LVH in the Moscow population

Polymorphism of Angiotensinogen II T174M Gene and Cardiovascular Diseases in the Moscow Population

Groups of patients with myocardial infarction (MI) and left ventricular hypertrophy (LVH) (n = 45 and n = 53, respectively) and a sample of healthy individuals from the Moscow population (n = 60) were examined for T174M polymorphism of AGT gene (replacement of methionine for threonine at position 174 of the correspondent amino acid sequence). In MI patients, the content of TT genotypes and T allele was significantly lower than in the control group (57.8 vs. 80% and 67.9 vs. 89.2%, respectively), whereas the proportion of M allele and TM heterozygotes was increased (32.1 vs. 10.8% and 37.8 vs. 18.3%, respectively). In patients with LVH, the proportion of TT genotype (64.2%) and T allele (77.4%) was also lower than in the control group, whereas the frequency of M allele was increased (22.6%). Our results suggest that the T174M polymorphism of AGT gene is associated with MI and LVH in the Moscow population

Association between polymorphism T174M of the angiotensinogen gene and arterial hypertension in muscovites

[No abstract available

Polymorphism of Angiotensinogen T174M Gene and Cardiovascular Diseases in the Moscow Population

The groups of patients with myocardial infarction (MI) and hypertrophy of the left ventricle (HLV) (n = 45 and n = 53, respectively) and a sample of healthy individuals from the Moscow population (n = 60) were examined for T174M polymorphism of ACT gene (replacement of methionine for threonine at position 174 of the correspondent amino acid sequence). In MI patients the content of 7T genotypes and 7 allele was significantly lower than in the control group (57.8% against 80% and 67.9 against 89.2%, respectively), whereas the proportion of M allele and TM hétérozygotes was increased (32.1 against 10.8% and 37.8 against 18.3%, respectively). In patients with HLV, the proportion of 7T genotype (64.2%) and T allele (77.4%) was also lower than in the control group, whereas the frequency of M allele was increased (22.6%). Our results suggest that the T174M polymorphism of ACT gene is associated with MI and HLV in the Moscow population