20 research outputs found

    Aphelenchoides bicaudatus from Ornamental Nurseries in Taiwan and Its Relationship with Some Agricultural Crops

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    Aphelenchoides bicaudatus nematodes were identified in planting materials and water samples from 35 floral ornamental nurseries in Taiwan. A pure line of the nematode was established from the bulk samples collected, and the nematode was identified as A. bicaudatus on the basis of morphometric data and the 18S gene sequence (GU984233). Host range tests were conducted by inoculating A. bicaudatus onto Phalaenopsis spp. strawberry, rice, bird’s-nest fern, cyclamen, dendrobium, African violet, and saxifrage (all reported hosts of A. besseyi). Symptoms did not develop during the 4 weeks following inoculation. However, after staining, both eggs and nematodes inside plant tissue of Phalaenopsis roots, strawberry leaf buds, and rice sheath indicated that A. bicaudatus had established in these hosts. These results revealed a potential new host–parasite relationship of A. bicaudatus, and suggest that this nematode may infect these economically important hosts

    Differentiation of the Xiphinema americanum-group nematodes X. brevicollum, X. incognitum, X. diffusum and X. oxycaudatum in Taiwan by morphometrics and nuclear ribosomal DNA sequences

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    Morphometrics and molecular variability of the X. americanum-group collected in Taiwan were studied. Morphometric data, derived from the females and their developing juveniles, revealed that the 27 populations comprised four taxa: X. brevicollum, X. incognitum, X. diffusum and X. oxycaudatum, the last species being found to have only three juvenile stages. Further identifications were conducted by analysing the nucleotide sequences of the first internal transcribed spacer (ITS-1), 5.8S gene and second internal transcribed spacer (ITS-2) of ribosomal DNA (rDNA). Pairwise comparisons revealed the sequence differences among these taxa ranging from 3.5 to 21.8% for the ITS-1, 2.9 to 28.6% for the ITS-2 and 0 to 3.1% for the 5.8S. Little or no intraspecific variation was detected in the four species for which multiple populations from different geographical regions or hosts were sequenced. Although X. incognitum is morphometrically closer to X. diffusum than it is to X. brevicollum in mean body length, total stylet length, and percentage position of vulva, there is a higher degree of genetic similarity between X. incognitum and X. brevicollum

    Effect of Streptomyces saraceticus Fertilizer on Plant Growth of 'Kyoho' Potted Grapevines

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    The effect of biofertilizer Streptomyces saraceticus added soybean meal and sugar on plant growth of ‘Kyoho'(Vitis vinifera L. x Vitis labruscana Bailey) grapevines were investigated in this study. Different treatments of S. saraceticus fertilizer was supplied in summer of 2005, and its effct on plant growth of potted young plants were investigated in growth, fresh weight , dry weight and N,P,K element uptake of shoot, trunk and roots. Results were shown that S. saraceticus fertilizer supply not only promoted shoot growth double than non-supply one, but also appeared in shoot diameter, trunk diameter and roots growth. It was also found that fresh weight, dry weight and nutrient elements in different part of potted plant increased significantly after S. saraceticus fertilizer supply. The material for improving growth in this biofertilizer were soybean meal and sugar.為探討S.saracetcius(放射線菌)添加黃豆粉及黑糖之肥料製劑對'巨峰'葡萄(Vitis vinifera L. × Vitis labruscana Baney cv. Kyoho)植株生長之影響。本研究於2005年夏季對'巨峰'葡萄盆栽植株施用不同配方之肥料製劑,觀察其對植株生長之影響。由調查結果得知,施用S.saracetcius肥料製劑可促進植株之生長,可促使秋季新梢生長達未處理之兩倍、加粗新梢橫徑及幹徑並促使根部生長。另外,施用S.saracetcius肥料製劑不僅使植株鮮、乾重增為對照組的l.5倍,且可促進植株吸收大量營養元素,造成植株體內之元素乾物量也較多,主要係黃豆粉及黑糖之作用

    The protein and genomic DNA sequences of <i>Abe GH5-1</i>.

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    <p>The Abe GH5-1 protein contains a SCP-like domain at the N-terminus and a GH5 cellulase domain at the C-terminus, as well as five introns were found in the <i>Abe GH5-1</i> gene.</p

    The gene structures of three GH45 genes in <i>Aphelenchoides besseyi</i>.

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    <p>The introns not to exist in the <i>Abe GH45-1</i> and presence in the <i>Abe GH45-2</i> and <i>Abe GH45-3</i> genes. Two introns, 163 bp and 142 bp, were found in the <i>Abe GH45-2</i> and a 46-bp sized intron was found in the <i>Abe GH45-3</i> gene.</p
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