Interplay between 15-lipoxygenase-1 and metastasis-associated antigen 1 in the metastatic potential of colorectal cancer

Objectives: Metastasis-associated antigen 1 (MTA1) is implicated in metastasis while 15-lipoxygenase-1 (15-LOX-1) reduces cell motility, when re-expressed in colorectal cancer (CRC). We aimed to understand any potential interplay between MTA1 and 15-LOX-1 in CRC metastasis. Materials and methods: ALOX15 and MTA1 expression in tumour and normal samples were analysed from TCGA RNA-seq data, microarray data sets and a human CRC cDNA array. Western blots, chromatin immunoprecipitation (ChIP), luciferase assays and electrophoretic mobility shift assays (EMSA) were carried out in HT-29 and LoVo cells re-expressing 15-LOX-1 to determine NF- κB activity at the MTA1 promoter. Functional assays in cells ectopically expressing either 15-LOX-1, MTA-1 or both, were carried out to determine adhesion and cell motility. Results: Significantly higher expression of MTA1 was observed in tumours compared to normal tissues; MTA1 overexpression resulted in reduced adhesion in CRC cell lines. Re-expression of 15-LOX-1 in the CRC cell lines reduced expression of endogenous MTA1, corroborated by negative correlation between the two genes in two independent human CRC microarray data sets, with greater significance in specific subsets of patients. DNA binding and transcriptional activity of NF-κB at the MTA1 promoter was significantly lower in cells re-expressing 15-LOX-1. Functionally, the same cells had reduced motility, which was rescued when they overexpressed MTA1, and further corroborated by expressions of E-cadherin and vimentin. Conclusions: Expression of MTA1 and 15-LOX-1 negatively correlated in specific subsets of CRC. Mechanistically, this is at least in part through reduced recruitment of NF-κB to the MTA1 promoter. © 2016 John Wiley & Sons Lt

An Investigation of the Effects of Ageing Parameters on Wear Behaviours and Electrical Conductivity of Cu-Co-Be Alloys

In this study, the effects of ageing parameters were investigated on wear behaviours and electrical conductivity of Cu-Co-Be alloy. The samples were quenched at room temperature after solution heat treated for 1 h. Then, ageing processes were applied for three different temperatures (470°C, 500°C, 530°C) and for three different ageing times (1, 2, 3 h). Microstructure examining, electrical conductivity and hardness measurements were carried out after ageing process. On the samples, which obtained the highest hardness values from hardness test (470°C), there were carried out wear tests with 1 m s¯¹ sliding speed under 30 N at four sliding distance (500-2000 m). Results clearly show that the highest hardness values were measured for samples aged at 470°C for 3 h and hardness values were decreased with increasing ageing temperature. The lowest weight loss values were evaluated for samples aged at 470°C for 3 h. The highest electrical conductivity values were measured at 500°C for 1 h aged samples

Heavy Metals in Soil and Sand from Playgrounds of Çanakkale City (Turkey), and Related Health Risks for Children

Children spend most of their time in playgrounds and, in parallel, constitute the social group most sensitive to contaminants. Here, we present the results of a comparative study of heavymetal contents between soils and sand from sandboxes obtained from playgrounds of Çanakkale city. Average contents of soils followed the order of iron (Fe) (12,901 mg kg−1) > manganese (Mn) (475 mg kg−1) > zinc (Zn) (58 mg kg−1) > copper (Cu) (28 mg kg−1) > nickel (Ni), chromium (Cr) (21 mg kg−1) > lead (Pb) (18 mg kg−1). Sand had lower contents, however, due to the reduction of particles size through prolonged use, and accumulation of Fe/Mn (oxyhydr)oxides on sand grains tended to diminish the differences between soils and sand. Through chemometric analysis, Cr and Ni were found to have a lithogenic origin, while the rest of metals were related to anthropogenic activities. Spatially, heavy-metal contents followed the pattern of the city’s sprawl. Risk estimates on children’s health showed that ingestion was the most important exposure route, followed by dermal contact and inhalation. Exposure of children to sand was of similar importance to that of soils. These findings are significant, as the contamination of sand has not yet received much attention compared to the soil of playgrounds. © 2022 by the authors. Licensee MDPI, Basel, Switzerland