The effect of omega-3 solution in the prevention of epidural fibrosis formation in experimental rabbit hemilaminotomy model

The aim of this experimental study was to evaluate the effect of omega-3 fatty acids solution in the prevention of the forming of peridural fibrotic tissue and adhesion in a rabbit hemilaminotomy model. This new experimental model was previously developed by the first author of this study. Twenty-one New Zealand white rabbits were used for this experiment. Seven of them were randomly selected as Control group and was named as Group I rabbits. The rabbits in this group were not operated and treated. The second group was Group II including 7 rabbits. This group was named as hemilaminotomy and untreated rabbits. Group III included 7 rabbits and named as Omega-3 treated group after hemilaminotomy procedure. In this group (Group III), 250 mg omega-3 solution was put in the hemilaminotomy area before closing the skin and fascia. Four weeks later from the operation rabbits were sacrificied by perfusion with 10% neutral buffered formalin solution. The lumbar spines were removed and immersed in 10% neutral buffered formalin for approximately 24 hours. Then each specimen was decalcified in 5% formic acid for approximately 3 weeks. Specimens were cut coronally for gross inspection. Peridural scar tissue on gross inspection in coronal sectioned specimens was graded into three grades. Grade I epidural scar tissue was scored with 1 point, Grade II with 2 point and Grade III with 3 point. The average score of hemilaminotomy sites without treatment was found as 2, 6 ± 0, 51. In the other hand the average score of hemilaminotomy sites with omega-3 fatty acids treatment was found as 1, 9 ± 0, 56. The difference between the treatment and non-treatment group was statistically significant. Omega-3 fatty acids solution seems an effective treatment modality in the prevention of peridural excessive scar tissue formation in the rabbit hemilaminotomy model. © 2010 OMU All rights reserved

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PubMed: 23298350[No abstract available

Beneficial effects of Ebselen on corrosive esophageal burns of rats

Aim: This study was evaluated to investigate the efficacy of Ebselen, which is an organoselenium compound and glutathione peroxidase mimic, on the prevention of stricture development after esophageal caustic injuries in the rat. Methods: Thirty healthy male Wistar albino rats were utilized in this study. The rats were randomly allotted into one of three experimental groups: group A (sham) animals were uninjured. Caustic esophageal burn was created by applying 1 ml of 37.5% NaOH to the distal esophagus. Group B rats were injured but untreated. Group C rats were injured and received Ebselen (10 mg/kg/day) via the oral route. Blood and tissue samples for the biochemical and histopathological analysis were taken all rats at the end (28th day) of the experiment. Oxidative stress is believed to play a role in the pathogenesis of corrosive esophageal burns. To assess changes in the cellular antioxidant defense system, we measured the activities of antioxidant enzymes (such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD), and catalase (CAT)) in esophagus homogenates. We also measured esophageal tissue malondialdehyde (MDA) levels, a marker of lipid peroxidation, to determine whether there is an imbalance between oxidant and antioxidant status. Efficacy of the treatment was assessed by measuring the stenosis index and histopathologic damage score and biochemically by determining tissue hydroxyproline content, lipid peroxidation and antioxidant enzyme levels. Results: The stenosis index in group B was significantly increased compared with group A and C (P < 0.05). The hydroxyproline level was significantly increased in group B compared with group A and C (P < 0.05). In group B, the histopathologic damage score was significantly higher than in group C (P < 0.05). Treatment with Ebselen decreased tissue hydroxyproline levels, histological damage, and the stenosis index. Caustic esophageal burn increased the lipid peroxidation and also decreased the antioxidant enzyme levels in group B. Ebselen treatments for 28 days decreased the elevated lipid peroxidation and also increased the reduced antioxidant enzyme levels. Live weights of the rats was significantly decreased in group B compared with group A and C (P < 0.05). Conclusion: It is concluded that Ebselen has a preventive effect in the development of fibrosis and decrease the lipid peroxidation, and increase the antioxidant defense system activity in an experimental model of corrosive esophagitis in rats. © 2005 Elsevier Ireland Ltd. All rights reserved

Dexmedetomidine alleviates vacuolization and necrosis in tubular epithelial cells induced by aortic cross-clamping

OBJECTIVE: Acute kidney injury (AKI) is one of the main causes of mortality in patients undergoing emergency surgery due to an abdominal aortic aneurysm. This study aimed to determine the potential nephroprotective characteristics of dexmedetomidine (DMD) for the establishment of a standard therapeutic method for AKI. MATERIALS AND METHODS: Thirty Spraque Dawley rats were allocated to 4 groups: control, sham, ischemia-reperfusion, and ischemia/reperfusion (I/R)+dexmedatomidine. RESULTS: Necrotic tubules, degenerative Bowman’s capsule and vascular congestion were observed in the I/R group. In addition, there was an increase in tissue malondialdehyde (MDA), interleukin (IL)-1 and IL-6 levels in tubular epithelial cells. In contrast, we observed decreased tubular necrosis, IL-1, IL-6 and MDA levels in the DMD treatment group. CONCLUSIONS: DMD has a nephroprotective effect against acute kidney injury resulting from I/R, which is related to aortic occlusion used in the treatment of ruptured abdominal aortic aneurysms

Is adalimumab protective in ischemia?reperfusion injury in lung?

Objective(s): Increasing cytokines and reactive oxygen species (ROS) during ischemia reperfusion (I?R) leads to the lung damage. Adalimumab (Ada) is a potent tumor necrosis factor?alpha (TNF??) inhibitor agent. We aimed to evaluate whether Ada would prevent the lung tissue from damage development over the I?R process. Materials and Methods: Twenty seven Wistar albino male rats were divided into three groups (each group had 9 rats). To the control group, only laparotomy procedure was carried out. For I?R group, first infrarenal abdominal aorta was cross?clamped during 2 hr, and then reperfusion was performed for 2 hr. To I?R+Ada group, first a single dose of 50 mg/kg Ada was given intraperitoneally and 5 days later, same I?R procedure was carried out. Results: Levels of TNF??, malondialdehyde (MDA), myeloperoxidase (MPO), endothelin?1 (ET?1) and caspase?3 enzyme activity of I?R group were higher than that of both I?R+ Ada [TNF?? (P=0.021), MDA (P=0.029), MPO (P=0.012), ET?1 (P=0.036, caspase?3 (P=0.007), respectively] and control group [TNF?? (P=0.008), MDA (P<0.001), MPO (P=0.001), ET?1 (P<0.001), caspase?3 (P<0.001), respectively]. In IR group, severe damage was detected by hematoxylin?eosin staining. This damage was found less severe in Ada treatment group. Conclusion: The release of cytokines and ET?1 in a large proportion after I?R injury, and generating of ROS in excessive quantity could cause severe damage in the lung tissue. Ada could be considered as a protective agent for lung tissue during I?R process. © 2015, Mashhad University of Medical Sciences. All rights reserved

Effects of infliximab against carbon tetrachloride-induced spleen toxicity in rats

OBJECTIVE: Carbon tetrachloride (CCl4) is a non-polar molecule used in industry in grain curing, insect-killing and especially in the production of chlorofluorocarbons. It is estimated that an average of 70,000 industry workers in Europe are exposed to this toxic compound. MATERIALS AND METHODS: Twenty-four male Sprague-Dawley rats were randomly allocated into four groups: control group (saline only, Group I), infliximab (INF) group (Group II), CCl4 group (Group III) and CCl4+INF group (Group IV). RESULTS: While there was an increase in the numerical density of CD3, CD68, and CD200R positive T lymphocytes and macrophages in the CCl4 administration group (p=0.000), this was not the case in the CCl4+INF administration group (p=0.000). CONCLUSIONS: TNF-α inhibitors have a protective effect against CCl4-induced spleen toxicity/inflammation as seen by the reduction in CD3, CD68, CD200R positive T lymphocytes and macrophages

The effect of imatinib administered in the prenatal period on testis development in rats

WOS: 000575471100001PubMed: 32990058Background: the purpose of this study was to examine the effects of exposure to imatinib in the prenatal period on testis development in rats. Methods: Although all the study groups received intraperitoneal imatinib on prenatal days 1-8, no pregnancy occurred in the Imatinib-80 group. Immunohistochemical analysis, TUNEL, c-kit and PDGF staining revealed no difference between the groups in terms of positivity scoring. Results: A significant decrease was detected in total sperm counts in the Imatinib-20 group compared to the control group, but the sperm count was higher in the Imatinib-60 group than in the Imatinib-20 group. At biochemical measurements, the drug increased oxidative stress in the testis and serum in the Imatinib-20 group, but caused a decrease in tissue in the Imatinib-60 group. Thiol measurements revealed a decrease in the testis and serum in the Imatinib-60 group, while an increase in serum measurements was observed in the Imatinib-40 group. Analysis revealed no difference between the groups in terms of protamine and histone gene expression levels in testis tissue exposed to imatinib. Conclusion: Our findings show that prenatal exposure to imatinib can lead to histopathological and biochemical changes in testis tissue, but that no adverse effect occurs in nuclear maturation of germ cells during spermiogenesis.Recep Tayyip Erdogan University, Rize, TurkeyRecep Tayyip Erdogan University [TDK-2017-825]The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Recep Tayyip Erdogan University, Rize, Turkey [TDK-2017-825]

Hepatic effects of ketamine administration for 2 weeks in rats

PubMed: 23386779The aim of the present study was to investigate the long-term and high-dose application of ketamine on the liver by employing histologic and biochemical methods. A total of 30 male rats were randomly assigned to control and four treatment groups (n: 6). Saline for control group and different doses of ketamine for four treatment groups (40, 60, 80 and 100 mg kg-1) were administered intraperitoneal twice a day for 2 weeks. Immunohistological staining, light and electron microscopy were used to study tissue specimens. Histopathological changes were more severe and diverse in groups 80 and 100 mg kg-1 day-1, and the least significant change was observed in groups 40 and 60 mg kg-1 day-1. The most important ultrastructural changes were seen in mitochondria and in the rough endoplasmic reticulum. The immunoreactivity of calcineurin was determined as different. Prolonged use of ketamine caused hepatocellualar toxicity and histological changes in hepatocytes in a dose-dependent manner in all experimental groups. © The Author(s) 2014.The all costs were funded by the authors

Comparison of the anti-inflammatory effect of resveratrol and leuprolide acetate in an experimental endometriosis model

30th Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE) -- JUN 29-JUL 02, 2014 -- Munich, GERMANYWOS: 000359745300114[No abstract available]European Soc Human Reprod & Embryo