Salivary myeloperoxidase and malondialdehyde are increased in patients exhibiting an asymptomatic mandibular impacted third molar

To determine whether saliva is a good means of evaluating concentrations of oxidative stress biomarkers, analyzing the correlation between concentrations in saliva and in follicular tissue, and to compare biomarker concentrations in patients with one asymptomatic mandibular impacted third molar (MITM) (before extraction) with a healthy control, and to determine how biomarkers are modified by extraction. 80 patients with one asymptomatic MITM and 80 healthy controls were included. Saliva samples were collected from all subjects (before extraction in the study group) to evaluate Myeloperoxidase (MPO) and Malondialdehyde (MDA) concentrations. Follicular tissues were obtained during surgery to measure biomarkers. One month after extraction, saliva samples were collected to assess changes of oxidative stress. Salivary MPO and MDA showed positive correlation with concentrations in follicular tissue (MPO: correlation coefficient=0.72, p=0.025; MDA: =0.92, p=0.001). Patients with asymptomatic MITMs showed higher salivary concentrations of oxidative stress biomarkers than healthy control subjects, with statistical significance for both MPO (p<0.001) and MDA (p<0.001). One month after extraction, salivary biomarkers decreased significantly in the study group (p<0.001). Salivary MPO and MDA are higher among patients with one asymptomatic MITM, but these levels decrease significantly one month after surgical extraction. The large decrease in oxidative stress biomarkers could justify third molar extraction despite the absence of symptoms

Estudio in vitro del efecto de la curcumina, el licopeno y la irradiación sobre el carcinoma oral de células escamosas

El carcinoma oral de células escamosas (COCE) es el más común en la cavidad oral. Actualmente está aumentando el uso de productos naturales como anticancerígenos. Nosotros, desarrollamos un estudio in vitro para evaluar el efecto de la curcumina, licopeno e irradiación sobre COCE. La curcumina y el licopeno fueron administrados a dosis de 3, 4.25, 5.50 y 6.75 µM sobre cultivos de la línea celular PE/CA-PJ15 de COCE irradiados a diferentes dosis (1, 2.5 y 5 Gy), seguido de la evaluación sobre los efectos en la viabilidad celular, apoptosis y migración tras 24, 48 y 72 horas. Nuestros resultados mostraron que la aplicación de curcumina o licopeno sobre PE/CA-PJ15 sin irradiación ejerció un efecto inhibidor en la viabilidad celular y la migración, así como un incremento de la apoptosis celular. Igualmente, en ambas variables, la curcumina y el licopeno tuvieron un efecto sinérgico al aplicar radiación.Oral squamous cell carcinoma is the most common malignancy of the oral cavity. Recently, there is increasing interest in using natural products as anticancer agents. We develop an in vitro study to evaluate the effect of curcumin, lycopene and irradiation upon oral squamous cell carcinoma. Curcumin and lycopene were administrated at doses of 3, 4.25, 5.50 and 6.75 µM in PE/CA-PJ15 oral squamous cell carcinoma cultures irradiated with different doses (1, 2.5 and 5 Gy), followed by evaluation of the effects upon cell viability, apoptosis and migration of the cells after 24, 48 and 72 hours of incubation. Our results showed that the application of curcumin or lycopene to the PE/CA-PJ15 tumor cells without irradiation exerted an inhibitor effect upon cell viability and cell migration and increased the cell apoptosis. Similarly, in both variables curcumin and lycopene had a synergistic effect by applying irradiation