Перспективы применения в нейрорегенеративной хирургии обогащенного тромбоцитами фибринового матрикса

Перспективы применения в нейрорегенеративной хирургии обогащенного тромбоцитами фибринового матрикс

Inhibition of Na(+),K(+)-ATPase and activation of myosin ATPase by calix[4]arene C-107 cause stimulation of isolated smooth muscle contractile activity

The discovery of compounds that might modify myometrial contractility is an important area of researches. In our previous experiments, we found that some representatives of macrocyclic compounds fami­ly – calix[4]arenes – can modify the enzymatic and transport activity of membrane-bound cation-transport ATP hydrolases. The aim of this work was to study and compare the effect of calix[4]arene C-107 on the enzymatic activities of Mg2+-dependent ATPases of the uterine smooth muscle, namely: ouabain-sensitive Na+,K+-ATPase, plasma membrane Ca2+-independent “basal” Mg2+-ATPase, ATPase of the actomyosin complex and myosin subfragment-1, with effect on the contractile activity of the myometrium. It was shown that calix[4]arene C-107 efficiently inhibited myometrium Na+,K+-ATPase (I50 = 54 ± 6 nM) selectively to other ATP-hydrolases of the plasma membrane and simultaneously activated the enzymatic activity of the myosin ATPase of smooth muscles (A50 = 9.6 ± 0.7 μM). Such reciprocal biochemical effects led to the stimulation of the smooth muscle contractile activity that was demonstrated by the tensometric method using different isolated smooth muscles. Calix[4]arene С-107 was shown to stimulate the increase of the tonic component of myometrium contractions induced by oxytocin, as well as contractions of the caecum muscles induced by high-potassium solution or acetylcholine, and to maintain increased tension for a long time. Thus, calix[4]arene C-107 is a prospective compound for enhancing the smooth muscle basal tone and/or contraction in case of hypotonic dysfunctions

Clinical case of de novo anaplastic ganglioglioma and current status of the problem

The authors report a rare case of de novo malignant ganglioglioma (WHO grade III) in a 26-year old female. The patient has complained of periodic feeling fear, anxiety, episodes of impaired consciousness with extremity muscle cramps sometimes followed by urination, as well as flashes before eyes. Computed tomography perfusion and magnetic resonance spectroscopy were carried out for differential diagnosis between different types of tumors. Stereotactic biopsy was performed for histological examination. High surgical risk became a contraindication to gross total resection of the tumor. The patient has received radiation therapy (Trilogy linear accelerator) in a total dose of 60 Gy. The tumor shrank significantly. In 7 months, monitoring MRT did not detect further growth of the tumor. The authors have analyzed the case and reviewed the existing literature data regarding gangliogliomas. Taking into account low prevalence of these tumors (0.4-1% of all brain tumors), especially of their malignant forms (3-10% of gangliogliomas), lack of sufficient data regarding prognostic factors, life expectancy, time of recurrence, lack of accurate indications for different methods of treatment (surgery, radiation, chemotherapy), these tumors still need further research that should also involve supplementary neuroimaging techniques and stereotactic biopsy

Diazepinone effect on liver tissue respiration and serum lipid content in rats with a rotenone model of Parkinson’s disease

Parkinson’s disease (PD) is a chronic and progressive age-related neurodegenerative disorder. Accumulation of α-synuclein aggregates, oxidative stress, mitochondrial dysfunction and lipid metabolism disturbance are thought to be the key violations at PD pathogenesis. Despite long-time research the causes of PD occurrence are not yet clear. We investigated the influence of diazepinon, a new derivative of benzodiazepine, on liver tissue respiration (LTR), serum lipid content and behavioral parameters of rats with modeled PD induced by intraperitoneal injections of 2.0 mg/kg rotenone (ROT) within 28 days. LTR was assessed using the polarograph LP-9. Blood samples for biochemical analysis were collected from the inferior vena cava. The behavioral parameters of rats were studied by the open field test. We showed that in rats with ROT – induced PD the coefficient of liver oxygen consumption was decreased by 33.5% (P < 0.001), the serum content of phospholipids, cholesterol, cholesterol esters, free fatty acids and triglycerides was reduced by 21.4% (P < 0.001), 28.8% (P < 0.001), 26.8% (P < 0.001), 30.3% (P < 0.01) and 41.5% (P < 0.001) respectively and the motor disorders were detected. Diazepinone application resulted in a full recovery of LTR, serum concentration of phospholipids, partial recovery of serum free fatty acids and triglycerides content and significant improvement of motor behavior. However diazepinone did not affect the reduced concentration of cholesterol and cholesterol esters in the serum of rats with simulated PD

Ethanol reforming in non-equilibrium plasma of glow discharge

The results of a detailed kinetic study of the main plasma chemical processes in non-equilibrium ethanol/argon plasma are presented. It is shown that at the beginning of the discharge the molecular hydrogen is mainly generated in the reaction of ethanol H-abstraction. Later hydrogen is formed from active H, CH2OH and CH3CHOH and formaldehyde. Comparison with experimental data has shown that the used kinetic mechanism predicts well the concentrations of main species at the reactor outlet.Comment: 16 pages, 8 figure

Комплексне лікування хворих з карпальним та кубітальним тунельними синдромами

The results of complex treatment of 46 parients with carpal and cubital tunnel syndromes were analyzed. In order to improve the postoperative reabilitaition program in addition to the standard postoperative management were used also Neuromidin. It promoted to recover of extremities function and the improvement of treatment results.Проанализированы результаты комплексного лечения 46 больных с карпальным и кубитальным туннельными синдромами. В целях совершенствования послеоперационной реабилитации дополнительно использовали препарат нейромидин. Это способствовало скорейшему восстановлению утраченных функций поврежденной конечности, улучшению результатов лечения больных.Проаналізовані результати комплексного лікування 46 хворих з карпальним (КТС) та кубітальним тунельними синдромами. З метою вдосконалення післяопераційної реабілітації додатково використований препарат нейромідин. Це сприяло більш швидкому відновленню втрачених функцій ураженої кінцівки, поліпшенню результатів лікування хворих

Поєднання ураження плечового сплетення та черепно-мозкової травми. Особливості діагностики та клінічні ознаки

Inspection results of 348 injured persons with the close brachial plexus injury, 90 from them — combinated with craniocerebral trauma are given. Brachial plexus injury character and levels are diagnosed; the optimum medical tactics choice in a view of all mixted traumas to the present time remains difficult and complicated problem. Difficulties in diagnosis of brachial plexus close injury establishment in a combination with craniocerebral trauma consist of differential diagnostics pathogeneses infringements of top finiteness movements — the central or peripheral type of a paralysis. The clinical picture of brachial plexus closed injury peculiarities was investigated with an estimation of symptoms value and methods of inspection.Изложены результаты обследования 348 пострадавших с закрытым повреждением плечевого сплетения (ПС), у 90 из них — в сочетании с черепно-мозговой травмой (ЧМТ). Диагностика характера, уровня повреждения ПС, выбор оптимальной лечебной тактики с учетом всех составных сочетанной травмы является сложной задачей. Сложность установления диагноза закрытого повреждения ПС в сочетании с ЧМТ обусловлена необходимостью дифференциальной диагностики нарушения движений в верхней конечности — центрального или периферического типа паралича. Изучены особенности клинических проявлений закрытого сочетанного повреждения ПС с оценкой значения симптомов и методов обследования. Разработана таблица дифференциальной диагностики монопареза (моноплегии) верхней конечности у больных с сочетанным повреждением ПС.Викладені результати обстеження 348 потерпілих з закритим пошкодженням плечового сплетення (ПС), з них 90 — в поєднанні з черепно-мозковою травмою (ЧМТ). Діагностика характеру, рівня пошкодження ПС, вибір оптимальної лікувальної тактики з огляду на всі складові поєднаної травми, є складною проблемою. Своєчасна діагностика травми ПС, кваліфіковане надання допомоги на різних етапах, вчасне раціональне диференційоване мікрохірургічне лікування дають можливість відновити як побутову, так і професійну працездатність у значної кількості хворих. Складність встановлення діагнозу закритого пошкодження ПС в поєднанні з ЧМТ зумовлена необхідністю диференційної діагностики розладів рухів у верхній кінцівці — центрального чи периферійного типу паралічу. Вивчені особливості клінічних проявів закритого поєднаного пошкодження ПС з оцінкою значення симптомів та методів обстеження. Розроблена таблиця диференційної діагностики монопарезу (моноплегії) верхньої кінцівки у хворих з поєднаним пошкодженням ПС

Сombination damage of brachial plexus and craniocerebral trauma. Peculiarity of diagnostics and clinical symptoms

Inspection results of 348 injured persons with the close brachial plexus injury, 90 from them — combinated with craniocerebral trauma are given. Brachial plexus injury character and levels are diagnosed; the optimum medical tactics choice in a view of all mixted traumas to the present time remains difficult and complicated problem. Difficulties in diagnosis of brachial plexus close injury establishment in a combination with craniocerebral trauma consist of differential diagnostics pathogeneses infringements of top finiteness movements — the central or peripheral type of a paralysis. The clinical picture of brachial plexus closed injury peculiarities was investigated with an estimation of symptoms value and methods of inspection

The comparative estimation of cryopreserved embryonic nervous cells and bone marrow stromal cells transplantation results in area of experimental epileptic center

The comparative estimation of experimental epilepsy surgical treatment results in experiment with application of cryopreserved embryonic nervous cells and bone marrow stromal cells, inducted in neuroblasts, was carried out. It is established, that the cryopreserved embryonic nervous cells and bone marrow stromal cells, inducted in neuroblasts, transplanted in area of epileptic center not only keep their viability, but also were capable to differentiate in neurons. The similar positive dynamic of biogenic amines maintenance in a brain stem was marked in the groups of animals, in which the transplantation of cryopreserved embryonic nervous cells and bone marrow stromal cells, inducted in neuroblasts, have been executed