Isolation and Inhibitory Activity of Angiotensin 1-Converting Enzyme Inhibitor in Enzymatic Hydrolyzates of Extracts from Skin and Bone of Croaker Argyrosomus argentatus

Inhibitors which inhibit the angiotensin l-converting enzyme (ACE) were prepared from trypsin digests of croaker extracts. The inhibitory activity of ACE detected in the trypsin degests of croaker extracts was fractionated into two major phosphopeptides fractions of P-1 and P-2 by gel filtration chromatography on sephadex G-50. The inhibition of ACE of the two kinds of phosphopeptides fractions of P-1 and P-2 was investigated in vitro. The IC_ values of p-1 and p-2 of phosphopeptides for ACE were 0.18 and 10.2mg protein/ml, respectively. In addition, the ACE inhibitor of P-1 and P-2 were further purified by ultrafiltration and by sephadex G-15 and G-25 chromatography. ACE inhibitory activity was fractionated into three major phosphopeptides fractions of P-1-1,P-1-2 and P-1-3 from the trypsin digests of the P-1,and into three major fractions of P-2-1,P-2-2 and P-2-3 from the trypsin digests of the P-2 by gel filtration rechromatography on sephadex G-15 and G-25,respectively. The inhibition of ACE of the six kinds of phosphopeptides fractions (P-1-1,P-1-2,P-1-3,P-2-1,P-2-2 and P-2-3) was analyzed in vitro. The IC_ values of P-1-1,P-1-2,P-1-3,P-2-1,P-2-2 and P-2-3 of phosphopeptides for ACE were 1.12,1.84,0.15,0.70,4.80 and 1.98mg protein/ml, respectively. The P-1-3 fraction had most inhibition activity and showed 0.15mg protein/ml inhibition against ACE at IC_ value. The amino acid compositions of the phosphopeptides fractions (P-1-1,P-1-2,P-1-3,P-2-1,P-2-2 and P-2-3) were characterized by relatively high percentage for Glu, Asp, Gly, Ala, Val, Leu, Tyr, Lys and Arg

Evi1 is essential for hematopoietic stem cell self-renewal, and its expression marks hematopoietic cells with long-term multilineage repopulating activity

A new mouse in which an IRES-GFP cassette is knocked-in to the Evi1 locus reveals that HSC long-term multilineage repopulating activity specifically segregates with expression of the Evi1 transcription factor

Effect of Electrolyzed Water on Washing

The detergency, that is the effect on removal of particle soil, on soiling prevention and on deodorization etc., of the electrolyzed water was compared with that of the pure water. The electrolyzed water, i.e. the alkaline ion liquid and the acid ion liquid, was produced using an electricity resolution production device. The wet artificially soiled cloths were washed at 40? in each liquid for 10 minutes by a Terg-O-tometer. Sodium dodecyl sulfate (SDS) was used as detergent. The removal efficiency of carbon black was demanded by applying an expression of Kubelka-Munk on the basis of the surface reflection rates of the test cloths. The soiling rates of the test pieces of white polyester were calculated on the surface reflection rates after they were washed with the wet artificially soiled cloths. The smoke of a cigarette (tar 12 mg, nicotine 1.0 mg) was absorbed by the test cloths of wool muslin in a glass container sealed up for 24 hours, and they were washed only with water afterwards. The stench of the specimens was measured with a smell meter, and the deodorant efficiency of the stench was calculated afterwards. The detergency was all good in order of the alkaline ion liquid, the pure water, the acid ion liquid. The property of dispersing solid particles of each liquid was dependent on the ingredients included in each liquid, the pH of the liquid and the hardness of water. The power of dispersing particles of the alkaline ion liquid was the biggest in three kinds of liquids used