14 research outputs found
PHYSICO-CHEMISTRY OF THE PROCESSES OF SULPHATOFORMATION WITH LIME STONES
The object of investigation: the heterogenous system CaCO*003 - CaO - SO*002 - SO*003 - O*002. The chemism of the processes of interaction SO*002 with the products of the calcite decay, the kinetics of the heterogenous reactions has been studied, the detailed description of analytics of the composition and localization of solid phases in the reaction zone has been given. The composition of the arising phases has been determined, the mechanism of the topochemical reactions of the sulphur oxides with calcite at high temperatures has been established. The mathematical model and the computer program, optimizind the phase composition in the multicomponent systems, depending on the conditions, has been developed. The application of the thermogravimetric method of analysis has been shown for the purposes of modeling of the processes of gases sulphur-cleaning. The method of evaluation of the porosity has been developed.Available from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio
IN VITRO ACTIVATION OF EARLY-STAGE APOPTOSIS OF T LYMPHOCYTES BY TRANSFERRING APOPTOTIC AUTOLOGOUS CELL CULTURE COMPONENTS IN PATIENTS WITH RHEUMATOID ARTHRITIS
Defects in programmed death of peripheral blood lymphocytes may result into autotolerance and chronic persistence of inflammatory reaction in rheumatoid arthritis. According to recent data, apoptosis is not an autonomous process, i.e., an excessive accumulation of cells in conjunction with a depleted medium leads to cellular excess, thus causing effect of cells already committed to apoptosis upon surrounding cells, by morphogenetic damage due to mechanical forces. Such effects are called compensatory proliferation or apoptosis-induced proliferation. Extracellular vesicles (ectosomes and apoptotic bodies) also have a negative effect on cultured cells, triggering their programmed apoptotic death. In turn, apoptosis can also be controlled by neighboring cells in non-autonomous manner.We conducted studies that allowed us to optimize methods aimed at the initiation of apoptotic cell death and to investigate the effects of apoptotic environment upon autologous cells under physiological conditions. The selected conditions in combination with a fluorescent labeling of lymphocytes and subsequent separate flow cytometric analysis allowed us to evaluate parameters of early apoptosis in subpopulations of peripheral blood T-lymphocytes in rheumatoid arthritis. In vitro studies of cells from the patients with rheumatoid arthritis allowed us to reveal a pronounced readiness of primary (CFSE) and secondary (CFSE+)-induced T lymphocytes for early apoptosis after stimulation with anti-CD3 antibodies. It was observed both against initial level of apoptosis, and when compared to cells induced for apoptosis. The obtained data suggest that stimulation of T lymphocytes with antibodies against CD3, and, as a result, an in vitro rise in cell proliferation rate leading to increased levels of early apoptosis not only among the cells directly receiving a proliferative stimulus, but also to increased effect of cellular and humoral components from anti-CD3-stimulated cultures upon normally proliferating lymphocytes.The transfer of an autologous apoptotic “aCD3” and dexamethasone-stimulated cultures, which were initially induced under conditions of cell overcrowding and medium exhaustion, was shown to activate the process of early apoptosis among normally proliferating cells. Glucocorticoids are known to serve as agents of cell death induced by activation. At pharmacological concentrations, glucocorticoids and their synthetic analogues stimulate endonucleases in activated lymphocytes. These enzymes destroy DNA in the internucleosomal regions thus resulting into cell apoptosis. The results obtained in present study suggest an opportunity of an in vitro early-stage apoptosis induction in T lymphocytes from the patients with rheumatoid arthritis, by means of cells subjected to activation-induced apoptosis
Comments on “Chemical driving force for dislocation motion in hydrogen embrittlement” by J. C. M. Li et al
УЛЬТРАЗВУКОВА АРХІТЕКТОНІКА ЩИТОПОДІБНОЇ ЗАЛОЗИ ПІСЛЯ ВИКОРИСТАННЯ ЗВАРЮВАЛЬНИХ ТЕХНОЛОГІЙ ПРИ ХІРУРГІЧНОМУ ЛІКУВАННІ
Objective - to study the state of the thyroid gland residual tissue and the features ofvascular perfusion by ultrasonographic and Doppler characteristics in the postoperativeperiod under use of a welding high-frequency coagulator in the surgical treatment.Material and methods. The possibility of electrocoagulation use of the thyroid tissue inthe last 5 years has been studied in 230 patients by means of the high-frequency coagulator ЕХВА - 350МС and ЕКВЗ-300 with frequency of 66 kHz and 440 kHz in mono- andbipolar modes. In all patients, electrocoagulation was performed to prevent bleeding, "ina dry field" using a pincer. Ninety-six patients after the thyroid gland surgery with tissuepreservation underwent ultrasonic Dopplerography on day 10, in 1, 6, 12 months. Thecapabilities of the apparatus allowed to get the image of the residual tissue, determine itssize, echo structure, the sizes of newly discovered residue inclusions in the thyroid.Results. The use of high-frequency electrocoagulation and soft tissue wound technologyprovides reliable hemostasis, reduces the main stage of the operation, and makes itpossible to avoid complications. Normalization of echostructure occurs during 1 year ofobservation and after a year is the most stable, taking into account the functionalhyperplasia.Conclusions. A clear idea of the course of changes in the thyroid residue makes itpossible to establish a post-operative diagnosis, to avoid diagnostic errors - diagnosticsof nodular goiter recurrences, autoimmune thyroiditis (focal form), neoplasia phenomena,and timely correction of treatment.Цель работы - изучить состояние остаточной ткани щитовидной железы и особенностей сосудистой перфузии по ультразвуковой и допплеровской характеристикам в послеоперационном периоде при использовании в хирургическом лечении сваривающего высокочастотного коагулятора.Материал и методы. В 230 больных за последние 5 лет изучили возможности использования электрокоагуляции щитовидной железы с использованием высокочастотного коагулятора ЕХВА - 350МС и ЕКВЗ-300 с частотой 66 кГц и 440кГц вмоно- и биполярных режимах. У всех больных электрокоагуляция использоваласьдля предупреждения кровотечения - "в сухом поле" с использованием пинцета. 96больным после операции на щитовидной железе с сохранением ткани проведенаультразвуковая допплерография на 10 сутки, через 1, 6, 12 месяцев. Возможностиаппаратов дали возможность получить изображение остаточной ткани, определить ее величину, эхоструктуру, размеры вновь выявленных в тироидном остаткевключений.Результаты. Использование технологий высокочастотной электрокоагуляции исваривания мягких тканей, обеспечивает надежный гемостаз, сокращает основной этап операции, дает возможность избегать развития осложнений. Нормализация эхоструктуры происходит на протяжении 1 года наблюдения и ужечерез год есть стабильной, с учетом функциональной гиперплазии.Вывод. Четкое представление о течении изменений в тиреоидном остатке даетвозможность установить послеоперационный диагноз, избегнуть диагностических ошибок - гипердиагностики рецидивов узлового зоба, аутоимунного тиреоидита (очаговай формы), явлений неоплазии, выполнить своевременную коррекциюлечения.Мета роботи - вивчити стан залишкової тканини щитоподібної залози та особливостей судинної перфузії за ультразвуковою і допплерівською характеристиками в післяопераційному періоді при використанні у хірургічному лікуваннізварювального високочастотного коагулятора.Матеріал і методи. У 230 хворих за останні 5 років вивчили можливості застосування електрокоагуляції щитоподібної залози з використанням високочастотного коагулятора ЕХВА - 350МС та ЕКВЗ-300 з частотою 66 кГц і 440кГц вмоно- та біполярному режимах. У всіх хворих електрокоагуляція виконувалася длязапобігання кровотечі - "в сухому полі" з використанням пінцета. 96 хворим післяоперації на щитоподібній залозі із збереженням тканини виконано ультразвуковудоплерографію на 10-ту добу, через 1, 6, 12 місяців. Спроможності апаратів дализмогу отримувати зображення залишеної тканини, визначити її величину, ехоструктуру, розміри знову виявлених у тиреоїдному залишку включень.Результати. Використання технологій високочастотної електрокоагуляції тазварювання м'яких тканин, забезпечує надійний гемостаз, скорочує основний етапоперації, дає можливість уникати ускладнень. Нормалізація ехоструктури відбувається протягом 1 року спостереження і вже через рік є найбільш сталою, зурахуванням функціональної гіперплазії.Висновки. Чітка уява про перебіг змін у тиреоїдному залишку дає можливістьвстановити післяопераційний діагноз, уникнути діагностичних помилок - гіпердіагностики рецидивів вузлового зобу, аутоімунного тиреоідиту (вогнищевоїформи), явищ неоплазії, виконати своєчасну корекцію лікування
On the kinetics of intergranular embrittlement of nickel by hydrogen transport from the external surface
Induction of lymphocyte apoptosis in healthy individuals and patients with rheumatoid arthritis under “cellular neighborhood” in vitro
The aim of the study was to investigate the features of T-lymphocyte apoptosis induced by components of autologous apoptotic cultures in vitro in norm and rheumatoid arthritis in the context of «cellular neighborhood».Materials and methods. Subjects of the study were blood samples of patients with rheumatoid arthritis (RA) and healthy women of comparable age. Developed protocol allowed to differentially evaluate the parameters of proliferation, early and late stages of apoptosis in the «primary» (CFSE-) and «secondary» (CFSE+) induced apoptotic T-lymphocyte cultures. It was estimated the effect of cellular and humoral components of unstimulated, anti-CD3- and dexamethasone-stimulated cells under the conditions of overcrowding and depleted culture media on autologous lymphocytes, cultured under physiological conditions, in norm and RA.Results. Comparative qualitative analysis revealed the features of the processes of T-lymphocyte apoptosis in norm and pathology. Also, the parameters of early and late stages of apoptosis of a «primary» induced culture and «secondary» induced cells after transferring the cellular and humoral components of apoptotic cultures did not differ significantly either initially or during culturing in both investigated groups. But it was a significant increase in the amount of living T-cells in «primary»-induced unstimulated and dexamethasone-stimulated RA patients’ cultures compared to similar donors’ cultures.Conclusion. There was no difference between stimulated with anti-CD3 antibodies cells and the «secondary» induced cultures. Taking into account the absence of significant differences in the parameters of activation apoptosis, the increased number of living cells in RA patients’ cultures relative to donors’ is evidence of contribution of non-autonomous apoptosis effects to cellular homeostasis in RA
Discussion on the Design Strategy of Urban landmark Space Based on Multi-perception Level
Риск развития диссеминированных процессов в легких у рабочих промышленных предприятий
There were discovered 7 cases of diffuse lung diseases (DLD) (5 — pneumoconiosis, 2 — fibrosing alveolitis) among 60 workers (welding and metal-polishing workers) with chronic non-specific lung diseases (CNLD). Five cases of pneumoconiosis were also found among 16 welding workers who worked indoors. Other 11 welding workers and 9 of 15 patients with chronic bronchitis without roentgenographic signs of DLD had similar changes in bronchoalveolar lavage fluid in comparison with patients having DLD changes. It was recomended to consider the large number of lymphocytes in lavage fluid as an evidence of the risk of the DLD development. The large number of neutrophyls, eosinophyls, T-4+ cells, the fagocytic activity of alveolar macrophages, and the activity of natural killers in lavage fluid could testify about the onset of DLD.Было обнаружено 7 случаев диффузных легочных заболеваний (ДЛЗ) (5 — пневмокониоз, 2 — фиброзирующий альвеолит) среди 60 рабочих (сварщики и шлифовщики металла) с хроническими неспецифическими заболеваниями легких (ХНЗЛ). Пять случаев пневмокониоза были также найдены среди 16 рабочих-сварщиков, работающих в помещении. Другие 11 рабочих-сварщиков и 9 из 15 пациентов с хроническим бронхитом без рентгенографических признаков ДЛЗ имели подобные изменения в бронхоальвеолярной лаважной жидкости по сравнению с пациентами, имеющими ДЛЗ изменения. Было рекомендовано к рассмотрению большое количество лимфоцитов в лаважной жидкости как доказательство риска развития ДЛЗ. Большое количество нейтрофилов, эозинофилов, Т-4 клеток, фагоцитарной активности альвеолярных макрофагов и активности естественных подавителей в лаважной жидкости могли свидетельствовать относительно начала ДЛЗ