16 research outputs found
Fluorescent Labeling of the Nuclear Envelope by Localizing Green Fluorescent Protein on the Inner Nuclear Membrane
The nuclear envelope (NE) is a double membrane that segregates nuclear components from the cytoplasm in eukaryotic cells. It is well-known that the NE undergoes a breakdown and reformation during mitosis in animal cells. However, the detailed mechanisms of the NE dynamics are not yet fully understood. Here, we propose a method for the fluorescent labeling of the NE in living cells, which enables the tracing of the NE dynamics during cell division under physiological conditions. In our method, labeling of the NE is accomplished by fixing green fluorescent protein carrying the nuclear localization signal on the inner nuclear membrane based on a unique biotinylation reaction from the archaeon Sulfolobus tokodaii. With this method, we observed HeLa cells during mitosis by confocal laser scanning microscopy and succeeded in clearly visualizing the difference in the timing of the formation of the NE and the nuclear lamina
Description of Bursaphelenchus conicaudatus n. sp. (Nematoda: Aphelenchoididae), isolated from the yellow-spotted longicorn beetle, Psacothea hilaris (Coleoptera: Cerambycidae) and fig trees, Ficus carica
Fluorescent Labeling of the Nuclear Envelope by Localizing Green Fluorescent Protein on the Inner Nuclear Membrane
The nuclear envelope (NE) is a double
membrane that segregates
nuclear components from the cytoplasm in eukaryotic cells. It is well-known
that the NE undergoes a breakdown and reformation during mitosis in
animal cells. However, the detailed mechanisms of the NE dynamics
are not yet fully understood. Here, we propose a method for the fluorescent
labeling of the NE in living cells, which enables the tracing of the
NE dynamics during cell division under physiological conditions. In
our method, labeling of the NE is accomplished by fixing green fluorescent
protein carrying the nuclear localization signal on the inner nuclear
membrane based on a unique biotinylation reaction from the archaeon <i>Sulfolobus tokodaii</i>. With this method, we observed HeLa
cells during mitosis by
confocal laser scanning microscopy and succeeded in clearly visualizing
the difference in the timing of the formation of the NE and the nuclear
lamina
Fluorescent Labeling of the Nuclear Envelope by Localizing Green Fluorescent Protein on the Inner Nuclear Membrane
The nuclear envelope (NE) is a double
membrane that segregates
nuclear components from the cytoplasm in eukaryotic cells. It is well-known
that the NE undergoes a breakdown and reformation during mitosis in
animal cells. However, the detailed mechanisms of the NE dynamics
are not yet fully understood. Here, we propose a method for the fluorescent
labeling of the NE in living cells, which enables the tracing of the
NE dynamics during cell division under physiological conditions. In
our method, labeling of the NE is accomplished by fixing green fluorescent
protein carrying the nuclear localization signal on the inner nuclear
membrane based on a unique biotinylation reaction from the archaeon <i>Sulfolobus tokodaii</i>. With this method, we observed HeLa
cells during mitosis by
confocal laser scanning microscopy and succeeded in clearly visualizing
the difference in the timing of the formation of the NE and the nuclear
lamina
Fluorescent Labeling of the Nuclear Envelope by Localizing Green Fluorescent Protein on the Inner Nuclear Membrane
The nuclear envelope (NE) is a double membrane that segregates nuclear components from the cytoplasm in eukaryotic cells. It is well-known that the NE undergoes a breakdown and reformation during mitosis in animal cells. However, the detailed mechanisms of the NE dynamics are not yet fully understood. Here, we propose a method for the fluorescent labeling of the NE in living cells, which enables the tracing of the NE dynamics during cell division under physiological conditions. In our method, labeling of the NE is accomplished by fixing green fluorescent protein carrying the nuclear localization signal on the inner nuclear membrane based on a unique biotinylation reaction from the archaeon Sulfolobus tokodaii. With this method, we observed HeLa cells during mitosis by confocal laser scanning microscopy and succeeded in clearly visualizing the difference in the timing of the formation of the NE and the nuclear lamina