Rhythmic Diel Pattern of Gene Expression in Juvenile Maize Leaf

Co-expressed genes belonging to individual clusters are likely to be regulated by common mechanisms. The nocturnal phase of the diurnal cycle involves gross induction of fundamental biochemical processes and should be studied more thoroughly than was appreciated in most earlier physiological studies. Although some general mechanisms responsible for the diel regulation of gene expression might be shared among plants, details of the diurnal regulation of gene expression seem to differ between taxa

Molecular foundations of chilling-tolerance of modern maize

Gene Ontology categories of GO class “Molecular Function” significantly over-represented among transcripts down-regulated by cold treatment in S50676 inbred line. (PDF 233 kb

Genome‑wide transcriptomic analysis of response to low temperature reveals candidate genes determining divergent cold‑sensitivity of maize inbred lines

Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive statistical elaboration of data, literature data mining, and gene ontology-based classification. The lines used had been bred earlier specifically for determination of QTLs for cold-performance of photosynthesis. This allowed direct comparison of present transcriptomic data with the earlier QTL mapping results. Cold-treated (14 h at 8/6 °C) maize seedlings of cold-tolerant ETH-DH7 and cold-sensitive ETH-DL3 lines at V3 stage showed strong, consistent response of the third leaf transcriptome: several thousand probes showed similar, statistically significant change in both lines, while only tens responded differently in the two lines. The most striking difference between the responses of the two lines to cold was the induction of expression of ca. twenty genes encoding membrane/cell wall proteins exclusively in the cold-tolerant ETH-DH7 line. The common response comprised mainly repression of numerous genes related to photosynthesis and induction of genes related to basic biological activity: transcription, regulation of gene expression, protein phosphorylation, cell wall organization. Among the genes showing differential response, several were close to the QTL regions identified in earlier studies with the same inbred lines and associated with biometrical, physiological or biochemical parameters. These transcripts, including two apparently non-protein-coding ones, are particularly attractive candidates for future studies on mechanisms determining divergent cold-tolerance of inbred maize lines.ISSN:0167-4412ISSN:1573-502

Child at risk of neglect from the view of social and legal protection of children in practice

Gene Ontology categories of GO class “Biological Process” significantly over-represented among transcripts up-regulated by cold treatment in S68911 inbred line. (PDF 247 kb

Additional file 24: of Molecular foundations of chilling-tolerance of modern maize

Possible functions and cellular localization of products of genes specific to S160 inbred line. (XLSX 168 kb

Additional file 26: of Molecular foundations of chilling-tolerance of modern maize

Primers for miRNA. (PDF 8 kb

Additional file 17: of Molecular foundations of chilling-tolerance of modern maize

Gene Ontology categories of GO class “Molecular Function” significantly over-represented among transcripts up-regulated by cold treatment in S160 inbred line. (PDF 174 kb