Inhibitory Effect of Helicteres gardneriana Ethanol Extract on Acute Inflammation

The anti-inflammatory effect of an ethanol extract of Helicteres gardneriana (Nees) Castiglioni was assayed in experimental models of pleurisy and microcirculation in situ. Treatment of animals with 500 mg/kg body weight reduced the exudate volume (35% reduction) induced by intrapleural injection of carrageenan and the migration of polymorphonuclear cells into the inflamed pleural cavity of rats (40%). Additionally, rolling and adhesion of leukocytes and the number of leukocytes that migrated toward the perivascular space in response to the carrageenan injection were decreased by the extract (500 mg/kg). These data demonstrate the anti-inflammatory effect of the ethanol extract of Helicteres gardneriana and imply that inhibition of leukocyte-endothelial interactions is important in the extract's mechanism of action

<b>Produtos em filme – Inovação na tecnologia de cosméticos</b> - DOI: 10.4025/actascihealthsci.v32i1.6987

A indústria cosmética tem investido fortemente na pesquisa e no desenvolvimento de novos produtos, para melhor atender as exigências do consumidor. Como inovação neste segmento têm despontado os cosméticos em filme. A partir de uma fórmula inicial, contendo como formador de película um polissacarídeo constituído de maltotriose e como agente gelificante sodium polyacrylate, foram obtidos géis viscosos que foram espalhados em placas de vidro, de maneira a se obter filmes de 1 mm de espessura. Após secagem à temperatura ambiente, os mesmos foram recortados (1 cm2) e acondicionados. Várias alterações foram realizadas a fim de se obter um filme flexível, com rigidez adequada, ausência de rachaduras e de bolhas, fácil dissolução em água e sensorial agradável. As formulações foram submetidas a diferentes temperaturas para avaliação preliminar da estabilidade. Foram desenvolvidos dois produtos, um contendo extratos hidroglicólicos de Aloe Vera e de Rosa Branca, auxiliares no tratamento da acne, e outro contendo extratos hidroglicólicos de Camomila e de Pera, auxiliares no combate a olheiras. Pequenos, leves e finos, os cosméticos em filme representam inovação que pode ocupar o lugar de produtos tradicionais, por proporcionarem maior praticidade e facilidade de uso e de transporte, mantendo a eficácia e a segurança necessárias a um cosmético

Produtos em filme – Inovação na tecnologia de cosméticos = Film products - Innovation in cosmetics technology

A indústria cosmética tem investido fortemente na pesquisa e no desenvolvimento de novos produtos, para melhor atender as exigências do consumidor. Como inovação neste segmento têm despontado os cosméticos em filme. A partir de uma fórmula inicial, contendo como formador de película um polissacarídeo constituído de maltotriose e como agente gelificante sodium polyacrylate, foram obtidos géis viscosos que foram espalhados em placas de vidro, de maneira a se obter filmes de 1 mm de espessura. Após secagem à temperatura ambiente, os mesmos foram recortados (1 cm2) e acondicionados. Várias alterações foram realizadas a fim de se obter um filme flexível, com rigidez adequada, ausência de rachaduras e de bolhas, fácil dissolução em água e sensorial agradável. As formulações foram submetidas a diferentes temperaturas para avaliação preliminar da estabilidade. Foram desenvolvidos dois produtos, um contendo extratos hidroglicólicos de Aloe Vera e de Rosa Branca, auxiliares no tratamento da acne, e outro contendo extratos hidroglicólicos de Camomila e de Pera, auxiliares no combate a olheiras. Pequenos, leves e finos, os cosméticos em filme representam inovação que pode ocupar o lugar de produtos tradicionais, por proporcionarem maior praticidade e facilidade de uso e de transporte, mantendo a eficácia e a segurança necessárias a um cosmético.<br><br>The cosmetics industry has invested heavily in the research and development of new products, to satisfy consumer demands. As a result of the innovation in this sector, cosmetics have emerged in film format. Starting from an initial formula containing a polysaccharide consisting of maltotriose units as a film former and sodium polyacrylate as gelling agent, viscous gels were obtained and dispersed in glass plate, in order to obtain 1 mm thick films. After drying to room temperature, they were cut out (1 cm2) and conditioned. Various alterations were carried out in order to obtain a film with good flexibility, adequate rigidity, without cracks and bubbles, easy dissolution in water and a pleasant feel. The formulations were submitted to different temperatures, for preliminary evaluation of their stability. Two products were developed: one containing hydroglicolic extracts from aloe vera and white rose, auxiliaries in the treatment of acne, and another containing hydroglicolic extracts from chamomile and of pear, used to combat dark circles under the eyes. Small, light and thin, film cosmetics are an innovation that can take the place of traditional products, as they offer greater practicality and ease of use and transport, maintaining the effectiveness and safety necessary for cosmetics

In vitro antibacterial activity of a 7-O-beta-D-glucopyranosyl-nutanocoumarin from Chaptalia nutans (Asteraceae)

Ethanolic crude extracts from the roots of Chaptalia nutans, traditionally used in Brazilian folk medicine, were screened against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa by using the disk diffusion test technique. S. aureus with 14 mm inhibition zone was considered susceptible. E. coli and P. aeruginosa without such a zone were considered resistant. As a result of this finding, the ethanolic crude extract was fractionated on silica gel column chromatography into five fractions. The ethyl acetate fraction was active against S. aureus and Bacillus subtilis. Further column chromatography separation of the ethyl acetate fraction afforded 30 fractions, which were assayed against S. aureus. Fractions 16 and 17 showed inhibition zones with S. aureus, indicating the presence of active compounds, and were subjected to purification by repeated preparative thin layer chromatography. The pure compound 7-O-beta-D-glucopyranosyl-nutanocoumarin inhibited B. subtilis and S. aureus at concentrations of 62.5 µg/ml and 125 µg/ml, respectively. The antibacterial property of C. nutans appears to have justified its use for the treatment of wounds, which are contaminated through bacterial infections

In Vitro Antibacterial Activity of a 7-O-b-D-glucopyranosylnutanocoumarin from Chaptalia nutans (Asteraceae)

Ethanolic crude extracts from the roots of Chaptalia nutans , traditionally used in Brazilian folk medicine, were screened against Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa by using the disk diffusion test technique. S. aureus with 14 mm inhibition zone was considered susceptible. E. coli and P. aeruginosa without such a zone were considered resistant. As a result of this finding, the ethanolic crude extract was fractionated on silica gel column chromatography into five fractions. The ethyl acetate fraction was active against S. aureus and Bacillus subtilis . Further column chromatography separation of the ethyl acetate fraction afforded 30 fractions, which were assayed against S. aureus . Fractions 16 and 17 showed inhibition zones with S. aureus , indicating the presence of active compounds, and were subjected to purification by repeated preparative thin layer chromatography. The pure compound 7-O- β -D-glucopyranosyl-nutanocoumarin inhibited B. subtilis and S. aureus at concentrations of 62.5 µ g/ml and 125 µ g/ml, respectively. The antibacterial property of C. nutans appears to have justified its use for the treatment of wounds, which are contaminated through bacterial infections

Inhibitory Effect of Helicteres gardneriana Ethanol Extract on Acute Inflammation

The anti-inflammatory effect of an ethanol extract of Helicteres gardneriana (Nees) Castiglioni was assayed in experimental models of pleurisy and microcirculation in situ. Treatment of animals with 500 mg/kg body weight reduced the exudate volume (35% reduction) induced by intrapleural injection of carrageenan and the migration of polymorphonuclear cells into the inflamed pleural cavity of rats (40%). Additionally, rolling and adhesion of leukocytes and the number of leukocytes that migrated toward the perivascular space in response to the carrageenan injection were decreased by the extract (500 mg/kg). These data demonstrate the anti-inflammatory effect of the ethanol extract of Helicteres gardneriana and imply that inhibition of leukocyte-endothelial interactions is important in the extract&apos;s mechanism of action

Development and evaluation of topical formulations that contain hydroethanolic extract from Schinus terebinthifolia against HSV-1 infection

The therapeutic drugs to treat&nbsp;Herpes simplex&nbsp;virus (HSV) infections have toxic side effects and there has been an emergence of drug-resistant strains. Therefore, the search for new treatments for HSV infections is mounting. In the present study, semi-solid formulations containing a crude hydroethanolic extract (CHE) from&nbsp;Schinus terebinthifolia&nbsp;were developed. Skin irritation, cutaneous permeation, and&nbsp;in vivo&nbsp;therapeutic efficacy of the formulations were investigated. Treatment with the ointment formulations did not result in any signs of skin irritation while the emulsions increased the thickness of the epidermis in Swiss mice. The cutaneous permeation test indicated that the CHE incorporated in the formulations permeated through the skin layers and was present in the epidermis and dermis even 3 h after topical application.&nbsp;In vivo&nbsp;antiviral activity in BALB/c mice treated with the CHE ointments was better than those treated with the CHE emulsions and did not significantly differ from an acyclovir-treated group. Taken together, this suggests that the incorporation of CHE in the ointment may be a potential candidate for the alternative topical treatment of herpetic lesions