ADENOSINE TRIPHOSPHATASE ACTIVITY IN BOVINE VASCULAR WALL

Histochemical investigation of both myosin and поп-myosin ATP-ases in bovine aorta, femoral artery and vein, vena cava caudalis and vena portae was carried out. The localization of these enzymes was determined by the structural peculiarities of the vascular wall and especially by the distribution of smooth muscle cells (SMC), the endotelium and vasa vasorum. In the outer part of aortic tunica media bundles of SMC and elastic lamellae were mozaically distributed. This fact determined the mozaic localization of enzymic activity in this layer. The reaction intensity in these SMC was higher than that in the inner media SMC and the intima as well the latter being similar in femoral artery SMC. There was a stronger reaction intensity in longitudinal adventitial SMC than that in the circular media layer when vena cava caudalis and vena portae were concerned. The intensity of ATP-ase reaction in SMC was proportional to the functional activity of these cells in the corresponding vascular wall layer

INTERMEDIATE FILAMENTS IN THE VASCULAR WALL: IMMUNOCYTOCHEMICAL STUDY

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AGE PECULIARITIES OF NON MYOSIN AND MYOSIN ADENOSINE TRIPHOSPHATASE ACTIVITY OF RABBIT VENOUS WALL

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AGE CHANGES OF ATP-ASE ACTIVITY IN HUMAN ARTERIAL AND VENOUS WALL

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Immunohistochemical Study Of Some Filamentous Proteins In The Cells Of Mature Human Umbilical Cord

Expression design of primary proteins from intermediate filaments such as cytokeratin, vimentin and desmin in any cells within the umbilical cord was immunohistochemically studied using polyclonal (PAN cytokeratin) and monoclonal (vimentin and desmin) antibodies. The results showed that the cells of the mature human umbilical cord such as amniocytes, cells of the mucilaginous connective tissue, endothelial and smooth-muscle vascular cells expressed the basic proteins of the intermediate filaments in a different way. The amniocytes reacted strongly positively towards cytokeratin while only single cells reacted towards desmin and vimentin. All  the cells of the mucilaginous connective tissue reacted positively for vimentin and desmin both. The vascular endothelial cells remained vimentin-positive only while the vascular myocytes demonstrated certain peculiarities of their reaction towards vimentin and desmin related not only with their vascular belonging (arterial or venous, respectively) but also with their intramural topography. Based on these new facts the authors discussed the nature, differentiation and functions of the structures involved in this important transitory formation

ADENOSINE TRIPHOSPHATASE ACTIVITY OF THE VENOUS WALL IN CAT

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Immunomorphological characteristics of pleomorphic adenoma of salivary glands

The immunohistochemical profile of 23 pleomorphic adenomas and 7 normal salivary glands was studied. We used antisera to vimentin (V), desmin (D), epithelial membrane antigen (EMA), prostate specific antigen (PSA), pancytokeratin, carcinoembryonic antigen (CEA), glial fibrillary acidic protein (GFAP) and S-100 protein. In the ducts and myoepithelial cells of normal salivary glands immunopositivity to most of the cytoskeletal proteins, EMA and CEA was observed. GFAP was localized only in cells of striated ducts. Major differences in the expression of various antigens among tubular structures, solid sheets, the myxoid and chondroid in the pleomorphic adenoma were encountered. Appearance of GFAP as a sign of stromal transformation into myxoid and chondroid was detected.Judging from these comparative immunohistochemical characteristics between normal salivary glands and pleomorphic adenomas, we assume that tumour cells originate from the reserve cells of intercalated and striated ducts.Nous avons étudié les caractéristiques immunohistochimiques de 23 adénomes pléomorphes et de 7 glandes salivaires non tumorales. Nous avons utilisé des anticorps pour la vimentine (V), la desmine (D), l’antigène de membrane épithéliale (EMA), antigène prostatique spécifique (PSA), la pancytokératine, l’antigène carcinoembryonnaire (CEA), la protéine gliale fibrillaire acide (GFAP) et la protéine S-100. Dans les canaux et dans les cellules myoépithéliales des glandes salivaires normales c’est l’immunopositivité pour la plupart des protéines du cytosquelette, EMA et CEA qui est observée. GFAP est localisée uniquement dans les cellules des canaux striés. Des différences majeures dans l’expression des divers antigènes ont été rencontrées dans les structures tubulaires, dans les nappes cellulaires et dans les portions myxoïdes et chondroïdes des adénomes pleomorphes. L’apparition de GFAP a été observée comme signe de transformation myxoïde ou chondroïde du stroma.En nous basant sur la comparaison des caractéristiques immunohistochimiques entre les glandes salivaires normales et les adénomes pléomorphes, nous supposons que les cellules tumorales trouvent leur origine dans les cellules de réserve des canaux intercalaires et des canaux striés.

SOME MORPHOLOGICAL PECULIARITIES OF THE LONG SAPHENOUS VEIN WITH OBLITERATING DISEASES OF THE LOWER LIMB

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Expression of cytoskeletal proteins and ATPase activity in bovine femoral artery and vein intima

Intima1 cells play an important role in the biology of the vascular wall. Variability in the metabolic activity of intimal smooth muscle cells (SMC), as well as the differential expression of cellular cytoskeletal proteins depend on factors such as degree of differentiation, aging, atherosclerosis, etc. Myosin ATPase activity and cytoskeletal proteins were studied in the intima of bovine femoral arteries and veins of mature animals. In some arteries the intima was thickened and two distinct layers - inner elastic hyperplastic (EHL) and outer, musculo-elastic (MEL) were observed. ATPase activity was well defined in endothelial cells (EC) as well as in SMC. However, differential enzymatic expression was observed in thickened intimas. SMC in the EHL were ATPase negative, while in the MEL they were ATPase positive. Al1 EC and SMC in the «normal» intimas were vimentin positive, desmin and cytokeratin negative. In vessels with thickened intimas, the EHL showed intensive vimentin positivity; in the MEL desmin immunoreactive SMC were numerous as were as those in the media. Vimentin-positive SMC occupied their innermost part. Differences in the expression of ATPase activity and cytoskeletal proteins is discussed in terms of possible migration of media1 SMC andlor morphological modulation observed in vessels with altered vascular walls