An implant periapical lesion associated with presence of residual root fragments: Report of case treated with apicoectomy

Indexación: Scopus.Chemicals and CAS Registry Numbers: azithromycin, 83905-01-5, 117772-70-0, 121470-24-4; chlorhexidine, 3697-42-5, 55-56-1; erythromycin, 114-07-8, 70536-18-4The present study reports a case of a periapical lesion in an implant placed in an alveolar ridge which presented a residual root fragment of a deciduous tooth. Patient attended a dental clinic because she wanted to replace the missing tooth with an implant. She reported having lost the temporary tooth for one year. The initial Cone-beam Computerised Tomography image showed an edentulous area in the #13 zone, and the presence of a fragment of the deciduous tooth. The small root fragment was removed and an implant 4.0 × 13 mm amplified was installed. Patient presented acute pain and inflammation at the implant site 20 d after the surgery when the implant was originally installed; the first periapical surgery was carried out. Four months after the first periapical surgery, in periapical x-ray a radiolucent lesion was observed surrounding the apical region of the implant with a dry sound under percussion. The second periapical surgery was carried out. Five months after the second periapical surgery, the presence of a fistula was observed clinically, while in X-ray a radiolucent lesion was observed in the apex of the implant. Implant-plasty was carried out, removing approximately 5 mm of the apical portion of the implant. This treatment was successful after 27 months' monitoring. © 2018, Scientific Publishers of India. All rights reserved.https://www.alliedacademies.org/articles/an-implant-periapical-lesion-associated-with-presence-of-residual-root-fragments-report-of-case-treated-with-apicoectomy-10489.htm

An MBO scheme for minimizing the graph Ohta-Kawasaki functional

We study a graph based version of the Ohta-Kawasaki functional, which was originally introduced in a continuum setting to model pattern formation in diblock copolymer melts and has been studied extensively as a paradigmatic example of a variational model for pattern formation. Graph based problems inspired by partial differential equations (PDEs) and varational methods have been the subject of many recent papers in the mathematical literature, because of their applications in areas such as image processing and data classification. This paper extends the area of PDE inspired graph based problems to pattern forming models, while continuing in the tradition of recent papers in the field. We introduce a mass conserving Merriman-Bence-Osher (MBO) scheme for minimizing the graph Ohta-Kawasaki functional with a mass constraint. We present three main results: (1) the Lyapunov functionals associated with this MBO scheme Γ-converge to the Ohta-Kawasaki functional (which includes the standard graph based MBO scheme and total variation as a special case); (2) there is a class of graphs on which the Ohta-Kawasaki MBO scheme corresponds to a standard MBO scheme on a transformed graph and for which generalized comparison principles hold; (3) this MBO scheme allows for the numerical computation of (approximate) minimizers of the graph Ohta-Kawasaki functional with a mass constraint

Transcriptome characterization by RNA sequencing identifies a major molecular and clinical subdivision in chronic lymphocytic leukemia

Chronic lymphocytic leukemia (CLL) has heterogeneous clinical and biological behavior. Whole-genome and -exome sequencing has contributed to the characterization of the mutational spectrum of the disease, but the underlying transcriptional profile is still poorly understood. We have performed deep RNA sequencing in different subpopulations of normal B-lymphocytes and CLL cells from a cohort of 98 patients, and characterized the CLL transcriptional landscape with unprecedented resolution. We detected thousands of transcriptional elements differentially expressed between the CLL and normal B cells, including protein-coding genes, noncoding RNAs, and pseudogenes. Transposable elements are globally derepressed in CLL cells. In addition, two thousand genes—most of which are not differentially expressed—exhibit CLL-specific splicing patterns. Genes involved in metabolic pathways showed higher expression in CLL, while genes related to spliceosome, proteasome, and ribosome were among the most down-regulated in CLL. Clustering of the CLL samples according to RNA-seq derived gene expression levels unveiled two robust molecular subgroups, C1 and C2. C1/C2 subgroups and the mutational status of the immunoglobulin heavy variable (IGHV) region were the only independent variables in predicting time to treatment in a multivariate analysis with main clinico-biological features. This subdivision was validated in an independent cohort of patients monitored through DNA microarrays. Further analysis shows that B-cell receptor (BCR) activation in the microenvironment of the lymph node may be at the origin of the C1/C2 differences

Evaluación físico-quimica y sensorial de frutos de uchuva (Physalis peruviana L.) Physico-chemical and sensory evaluation of cape gooseberry fruits (Physalis peruviana L.)

Se cosechan frutos de uchuva en grado de madurez de consumo en tres tiempos diferentes, se caracterizan físico-química y sensorialmente. Se realizan análisis de medidas repetidas con estructura de varianzascovarianzas autoregresiva heterogénea de primer orden, estableciendo diferencias estadísticamente significativas (p0,05) para las variables aroma, textura bucal y sabor. Para el programa de fitomejoramiento las características físico-químicas y organolépticas de las accesiones estudiadas son similares al testigo comercial; por lo tanto, se espera que los cruzamientos entre ellas no demeriten estos aspectos.Cape gooseberry fruits are harvested at maturity for consumption at three different times. They are evaluated physico-chemically and sensorially. Repeated measures analysis are performed using a variancecovariance structure of the first order autoregressive heterogeneous. The results show statistically significant differences (p0,05) for aroma, texture, mouth taste and flavors variables. The accesions studied for the phytoimprovement program of the physical, chemical and organoleptic characteristics were similar to the commercial samples. Therefore it is expected that crosses between them do not invalidate the exercise

Altitude and fertilization type: concentration of nutrients and production of biomass in Stevia rebaudiana Bertoni

Agricultural production systems demand techniques that allow reducing uncertainty in decision-making. A factor that adds uncertainty in agricultural systems is altitude since it influences the nutrient uptake by plants. This work aimed to evaluate the influence of two altitudinal gradients (2569 and 1487 m.a.s.l.) and two fertilizers: an organo-mineral fertilizer (composted poultry manure mixed with inorganic fertilizer) and mineral fertilizer (control) on the nutrient uptake and biomass production in commercial stevia crops. The effect of altitude and fertilizers was evaluated after 60 days, under greenhouse conditions, assessing macro and micronutrient levels in the plant leaves (nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, manganese, zinc, iron, and boron) and biomass production. The results of this work showed the effect of altitude (2569 m.a.s.l.) and organo-mineral fertilization on crop productivity and yield. The application of the organo-mineral fertilizer decreased the differences of nutrient uptake between the altitudinal gradients and provided both better nutrient uptake levels, mainly of N (79%), Ca (115%), and Mg (162%), and increased biomass production (49%), than mineral fertilization at both experimental sites. Likewise, the organo-mineral fertilizer enhanced the soils’ cation exchange capacity, increased nutrient availability, and minimizing the risks of contamination by nutrient leaching. An important conclusion of this work is that stevia plants grown at different altitudes differ in nutrient uptake (mostly nitrogen 79%); therefore, fertilizer dosing must be adjusted to crop altitude, especially for mineral fertilizers. Thus, fertilizer design should consider not only the plant nutrition requirements but also its environmental conditions. © 2020 Taylor & Francis Group, LLC

Clinical effect of driver mutations of JAK2, CALR, or MPL in primary myelofibrosis.

We studied the impact of driver mutations of JAK2, CALR, (calreticulin gene) or MPL on clinical course, leukemic transformation, and survival of patients with primary myelofibrosis (PMF). Of the 617 subjects studied, 399 (64.7%) carried JAK2 (V617F), 140 (22.7%) had a CALR exon 9 indel, 25 (4.0%) carried an MPL (W515) mutation, and 53 (8.6%) had nonmutated JAK2, CALR, and MPL (so-called triple-negative PMF). Patients with CALR mutation had a lower risk of developing anemia, thrombocytopenia, and marked leukocytosis compared with other subtypes. They also had a lower risk of thrombosis compared with patients carrying JAK2 (V617F). At the opposite, triple-negative patients had higher incidence of leukemic transformation compared with either CALR-mutant or JAK2-mutant patients. Median overall survival was 17.7 years in CALR-mutant, 9.2 years in JAK2-mutant, 9.1 years in MPL-mutant, and 3.2 years in triple-negative patients. In multivariate analysis corrected for age, CALR-mutant patients had better overall survival than either JAK2-mutant or triple-negative patients. The impact of genetic lesions on survival was independent of current prognostic scoring systems. These observations indicate that driver mutations define distinct disease entities within PMF. Accounting for them is not only relevant to clinical decision-making, but should also be considered in designing clinical trials