Investigation of mTOR-dependent neuronal markers, gene expression and central metabolism in dopaminergic neurons

The mechanistic target of Rapamycin (mTOR) signalling pathway is conserved in all eukaryotes and regulates growth and metabolism in response to environmental cues such as nutrients and growth stimuli. The heart of the pathway is the TOR kinase that operates within two functionally and structurally distinct complexes, TORC1 and TORC2. Analyses of transcriptomics data from previous experiments have identified transcription factors linked to development/metabolism i.e., LHX9 and GATA4, showing significant change in expression following pharmacological inhibition of mTOR via an ATP-competitive inhibitor of both TOR complexes, Torin1. Lim Homeobox 9 (LHX9), a transcription factor, is crucial for nervous system development and is implicated in glycolysis. The purpose of this research was to define the function of the mTOR pathway in controlling gene expression programmes during differentiation, growth and neurodegeneration as well as its implications into glycolysis by implementing a combination of pharmacological interventions in a time course manner using Torin1 (TORC1/2 inhibitor) and RapaLink-1 (TORC1 inhibitor), followed by an investigation into the role of LHX9 in mediating mTOR-dependent effects during growth and neurodegeneration. Western blotting was used to validate RNA-seq derived outputs by examining LHX9 expression in differentiated SH-SY5Y cells. The results show an upregulation of LHX9 in differentiated SH-SY5Y cells after Rapalink-1 and Torin1 treatments, with an increased expression with TORC1 and TORC2 inhibition. All the glycolytic enzymes tested did not have significant changes in differentiated SH-SY5Y cells with Torin1 and RapaLink-1 treatment showing that glycolysis runs normally. Due to these results, we can assume that LHX9 does not have an effect on glycolysis

Programa de salud: combatir la ansiedad desde la consulta de enfermería

Introducción. Se estima que el 30% de las consultas en atención primaria acuden por algún problema relacionado con un trastorno mental. Está demostrado que a largo plazo el tratamiento psicológico presenta mejores efectos que el farmacológico. Y no crea adicciones. El impacto socio laboral de los trastornos de ansiedad es muy importante, reflejado en un elevado coste sanitario y un mayor absentismo laboral. Objetivos. El objetivo de este programa de educación para la salud es evaluar la investigación científica recomendada para conseguir disminuir la problemática de las personas que sufren ansiedad, así como las crisis y los efectos que éstas provocan. Metodología. La búsqueda bibliográfica se realizó durante los meses diciembre – marzo en bases de datos como: PubMed, Cuiden, LILACS; recursos de evidencias como la Biblioteca Cochrane, JBI COnNECT+, Epistemonikos; bases de datos de guías clínicas como National Guideline Cleringhouse. Los criterios de inclusión han sido: artículos completos que engloben a personas con trastornos de ansiedad en la edad adulta, artículos en español, inglés y también en italiano. Desarrollo. El programa de salud planteado pretende proporcionar a través de la educación sanitaria y las técnicas de relajación un adecuado conocimiento sobre la ansiedad y controlar y/o disminuir sus síntomas. Esto se consigue implementando las intervenciones enfermeras en un contexto de asistencia grupal. Conclusiones. La literatura científica analizada sobre aspectos de ansiedad, define y posiciona a las intervenciones enfermeras como eficaces, reconociendo sus efectos favorecedores en la salud mental del individuo. Palabras Clave: Psicoterapia, atención ambulatoria, atención de enfermería, atención primaria de salud, terapias complementarias, trastornos de ansiedad, trastornos mentales, tratamiento farmacológico.<br /

Fosfomycin Trometamol (Monural®3g) in empiric treatment of the low urinary infection in female

Catedra Urologie şi Nefrologie Chirurgicala USMF „N.Testemiţanu”, Secţia Urologie IMSP Spitalul Clinic Republican, Spitalul Raional Cahul, Spitalul Raional Drochia, Al V-lea Congres de Urologie, Dializă şi Transplant Renal din Republica Moldova cu participare internaţională (1-13 iunie 2011)Summary. This study was conducted to assess the clinical and microbiological efficacies of a single 3-g dose of fosfomycin trometamol for the treatment of cystitis in women. 77,6% of pathogens were Escherichia coli. A clinical evaluation at the 5-7 day follow-up showed that 100% of the women were free of symptoms, the microbiological evaluation at the 5-7 day of follow-up showed a 96% eradication rate. A microbiological evaluation at 1 month demonstrated prolonged eradication in 92% women. Only in one case were reported adverse reactions. Fosfomycin trometamol in a single 3-g dose is effective in the treatment of cystitis

Alternativă contemporană în tratamentul bolilor sexual transmisibile la bărbați (rezultate preliminare)

Summary The study was performed on the basis of the data accumulated while applying antibacterial treatment for 69 patients with sexual transmitted disease. The results showed the increased efficiency of clatithromycine in treatment of chronic uretritis caused by Chi. Trachomatis, Ur. Urealythicum and Мус. Hominis

Signification of the international prostate symptom score (IPSS) and quality of life (QOL) in management of patients with BPH

Catedra Urologie şi Nefrologie Chirurgicala USMF „N.Testemiţanu” Secţia Urologie IMSP Spitalul Clinic Republican, Spitalul Raional Cahul, Spitalul Raional Drochia, Al V-lea Congres de Urologie, Dializă şi Transplant Renal din Republica Moldova cu participare internaţională (1-13 iunie 2011)Summary. The objective of this study was to determine signification of the International Prostate Symptom Score (IPSS) in management of patients with Benign Prostatic Hyperplasia. 90 patients with enlarged prostate and LUTS were evaluated by the International Prostate Symptom Score (IPSS) and urodynamics before and after surgical treatment. There was significant correlation of the IPSS and Uroflowmetry results. IPSS test is useful in the estimation of disease severity and results of surgical therapy in BPH patients

Adenoprosin efficacy in benign prostate hyperplasia treatment

Catedra de Urologie şi Nefrologie Chirurgicală USMF ”N. Testemiţanu”, Secţia Urologie IMSP Spitalul Clinic Republican, Spitalul raional Drochia, Spitalul raional Cahul, Al V-lea Congres de Urologie, Dializă şi Transplant Renal din Republica Moldova cu participare internaţională (1-13 iunie 2011)Summary. Conservative treatment occupies new positions in the therapy of BPH. This is due to several factors, including the increasing number of elderly patients and increasing of their associated diseases (60-90%), which does not allow radical treatment. Efficacy of conservative treatment is already proven by many clinical investigations, which induces an increased popularity of this method of treatment among urologists. The purpose of conservative treatment is not only to defer surgery in time, but is to obtain a high effect of sclerosis of prostate adenoma, and thereby cancellation of the surgery. Currently, pharmaceutical companies come with new and new groups of drugs, most effective in the treatment of BPH. One direction of future therapy in the treatment of BPH is bio-regulatory therapy with suppositories “Adenoprosin”, which is used successfully in the pharmacological treatment of prostate adenoma complicated with inflammatory processes such as acute and chronic prostatitis

The results of multicentric study of prostate pathology in some localities of Republic of Moldova

IP USMF "Nicolae Testemiţanu", IMSP Spitalul Clinic Republican, IMSP Spitalul Republican ACSR, IMSP SR Cahul, IMSP SR Edineţ, IMSP SR Drochia, IMSP SR Orhei, Compania farmaceutică Berline-Chemie Menarini, Reprezentanţa în R. Moldova, Al VI-lea Congres de Urologie, Dializă şi Transplant Renal din Republica Moldova cu participare internaţională (21-23 octombrie 2015)Rezumat. în lucrare sunt analizate rezultatele unui studiu multicentric a patologiei prostatei efectuat în baza screening-ului la bărbaţi din 7 localităţi ale R. Moldova. Conform protocolului adoptat au fost examinaţi 508 pacienţi cu vârsta medie de 63,3±9,1 ani. Au fost studiate structura patologiilor depistate (adenom de prostată (HBP), cancer de prostată (CP), prostatită), particularităţile clinice ale HBP, incidenţa CP, metodele de tratament recomandate în dependenţă de rezultatele screening-ului. Cea mai frecventă patologie a constituit HBP - 425 cazuri, sau 83,7%, în 8 cazuri (1,6%) a fost diagnosticat CP, iar prostatita cronică la 62 (12,2%) pacienţi. Majoritatea pacienţilor cu HBP - 76,8% au primit indicaţii pentru tratament conservator, 9,1% din pacienţi pentru prostatită cronică; tratamentul chirurgical a fost indicat la 30 pacienţi (5,9%) pentru HBP, iar tratament specializat pentru CP s-a indicat la 3 pacienţi (0,6%). La 12 pacienţi a fost necesar de efectuat o serie de investigaţii suplimentare pentru diferenţierea diagnosticului dintre adenom şi cancer de prostată.Summary. In article were analyzed the results of a multicenter screening study of the prostate pathology in men from 7 regions of R. of Moldova. According to the adopted protocol were examined 508 patients with a mean age of 63,3 ± 9,1 years. The structure of the identified pathologies (binign prostatic hyperplasia (BPH), prostate cancer (PC), prostatitis, the clinical features of BPH, the incidence of PC, the recommended treatment methods depending on the results of the screening were studied. The most frequent pathology was BPH - 425 cases or 83,7%, PC was diagnosed in 8 patients (1,6%) and chronic prostatitis in 62 (12,2%) patients. Most of the patients with BPH — 76,8% received indications for conservative treatment, 9,1% of patients received treatment for chronic prostatitis. Surgical treatment was indicated in 30 patients (5,9%) with BPH and specialized treatment for PC was indicated in 3 patients (0,6%). In 12 patients it was necessary to perform a number of additional investigations for differential diagnosis of adenoma and prostate cancer

Interplays of AMPK and TOR in Autophagy Regulation in Yeast

Cells survey their environment and need to balance growth and anabolism with stress programmes and catabolism towards maximum cellular bioenergetics economy and survival. Nutrient-responsive pathways, such as the mechanistic target of rapamycin (mTOR) interact and cross-talk, continuously, with stress-responsive hubs such as the AMP-activated protein kinase (AMPK) to regulate fundamental cellular processes such as transcription, protein translation, lipid and carbohydrate homeostasis. Especially in nutrient stresses or deprivations, cells tune their metabolism accordingly and, crucially, recycle materials through autophagy mechanisms. It has now become apparent that autophagy is pivotal in lifespan, health and cell survival as it is a gatekeeper of clearing damaged macromolecules and organelles and serving as quality assurance mechanism within cells. Autophagy is hard-wired with energy and nutrient levels as well as with damage-response, and yeasts have been instrumental in elucidating such connectivities. In this review, we briefly outline cross-talks and feedback loops that link growth and stress, mainly, in the fission yeast Schizosaccharomyces pombe, a favourite model in cell and molecular biology

Interplays of AMPK and TOR in Autophagy Regulation in Yeast

Cells survey their environment and need to balance growth and anabolism with stress programmes and catabolism towards maximum cellular bioenergetics economy and survival. Nutrient-responsive pathways, such as the mechanistic target of rapamycin (mTOR) interact and cross-talk, continuously, with stress-responsive hubs such as the AMP-activated protein kinase (AMPK) to regulate fundamental cellular processes such as transcription, protein translation, lipid and carbohydrate homeostasis. Especially in nutrient stresses or deprivations, cells tune their metabolism accordingly and, crucially, recycle materials through autophagy mechanisms. It has now become apparent that autophagy is pivotal in lifespan, health and cell survival as it is a gatekeeper of clearing damaged macromolecules and organelles and serving as quality assurance mechanism within cells. Autophagy is hard-wired with energy and nutrient levels as well as with damage-response, and yeasts have been instrumental in elucidating such connectivities. In this review, we briefly outline cross-talks and feedback loops that link growth and stress, mainly, in the fission yeast Schizosaccharomyces pombe, a favourite model in cell and molecular biology

Interplays of AMPK and TOR in Autophagy Regulation in Yeast

Cells survey their environment and need to balance growth and anabolism with stress programmes and catabolism towards maximum cellular bioenergetics economy and survival. Nutrient-responsive pathways, such as the mechanistic target of rapamycin (mTOR) interact and cross-talk, continuously, with stress-responsive hubs such as the AMP-activated protein kinase (AMPK) to regulate fundamental cellular processes such as transcription, protein translation, lipid and carbohydrate homeostasis. Especially in nutrient stresses or deprivations, cells tune their metabolism accordingly and, crucially, recycle materials through autophagy mechanisms. It has now become apparent that autophagy is pivotal in lifespan, health and cell survival as it is a gatekeeper of clearing damaged macromolecules and organelles and serving as quality assurance mechanism within cells. Autophagy is hard-wired with energy and nutrient levels as well as with damage-response, and yeasts have been instrumental in elucidating such connectivities. In this review, we briefly outline cross-talks and feedback loops that link growth and stress, mainly, in the fission yeast Schizosaccharomyces pombe, a favourite model in cell and molecular biology