Platelet-mediated metabolism of the common dietary flavonoid, quercetin.

BACKGROUND: Flavonoid metabolites remain in blood for periods of time potentially long enough to allow interactions with cellular components of this tissue. It is well-established that flavonoids are metabolised within the intestine and liver into methylated, sulphated and glucuronidated counterparts, which inhibit platelet function. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrate evidence suggesting platelets which contain metabolic enzymes, as an alternative location for flavonoid metabolism. Quercetin and a plasma metabolite of this compound, 4'-O-methyl quercetin (tamarixetin) were shown to gain access to the cytosolic compartment of platelets, using confocal microscopy. High performance liquid chromatography (HPLC) and mass spectrometry (MS) showed that quercetin was transformed into a compound with a mass identical to tamarixetin, suggesting that the flavonoid was methylated by catechol-O-methyl transferase (COMT) within platelets. CONCLUSIONS/SIGNIFICANCE: Platelets potentially mediate a third phase of flavonoid metabolism, which may impact on the regulation of the function of these cells by metabolites of these dietary compounds

Development of a 3D printer using scanning projection stereolithography

We have developed a system for the rapid fabrication of low cost 3D devices and systems in the laboratory with micro-scale features yet cm-scale objects. Our system is inspired by maskless lithography, where a digital micromirror device (DMD) is used to project patterns with resolution up to 10 µm onto a layer of photoresist. Large area objects can be fabricated by stitching projected images over a 5cm2 area. The addition of a z-stage allows multiple layers to be stacked to create 3D objects, removing the need for any developing or etching steps but at the same time leading to true 3D devices which are robust, configurable and scalable. We demonstrate the applications of the system by printing a range of micro-scale objects as well as a fully functioning microfluidic droplet device and test its integrity by pumping dye through the channels

Raising the bar: legislating to achieve diversity in the professions is only half of the story

Purpose – Argues that merely complying with legislation is not sufficient to accomplish genuine diversity and that, rather than assuming its benefits will naturally be derived and accepted, diversity must be approached strategically and its advantages communicated effectively to stakeholders to ensure desired outcomes. Design/methodology/approach – Considers the business case for diversity, presents a case study of barristers and examines the role of the national equality standard. Findings – Advances the view that multi-layered approaches to diversity are essential for its successful implementation. Practical implications – Highlights the strong business case for diversity in the workforce, as well as possible social repercussions from failing to ensure that workforces are diverse. Social implications – Underlines how young people in European and Anglo-Saxon countries are increasingly demanding to work with colleagues from varied ethnicities, nationalities and sexual orientations who are similar to the peers they socialize with. Diverse workforces have thus become central to talent attraction. Originality/value – Presents an interesting case study of barristers and diversity. Keywords: Performance, Recruitment, Equal opportunities, Lawyer

Priorities and Principles for Investment in Aquaculture Research by NSW Department of Primary Industries

This review examined the characteristics of the main aquaculture industries in NSW with respect to current impediments to growth, market development and future opportunities. Within this context, it examined the nature, funding and impacts of the NSW Department of Primary Industries’ current and proposed investments in aquaculture R&D and industry development, as well as its alignment with DPI and industry priorities.aquaculture, research evaluation, public good, Agribusiness, Livestock Production/Industries, Research and Development/Tech Change/Emerging Technologies, Q160,

Chemical characterisation and determination of sensory attributes of hydrolysates produced by enzymatic hydrolysis of whey proteins following a novel integrative process

The overall aim of this work was to characterize the major angiotensin converting enzyme (ACE) inhibitory peptides produced by enzymatic hydrolysis of whey proteins, through the application of a novel integrative process. This process consisted of the combination of adsorption and microfiltration within a stirred cell unit for the selective immobilization of β-lactoglobulin and casein derived peptides (CDP) from whey. The adsorbed proteins were hydrolyzed in-situ which resulted in the separation of peptide products from the substrate and fractionation of peptides. Two different hydrolysates were produced: (i) from CDP (IC50 =287μg/mL) and (ii) from β-lactoglobulin (IC50=128μg/mL). IC50 is the concentration of inhibitor needed to inhibit ACE by half. The well known antihypertensive peptide IPP and several novel peptides that have structural similarities with reported ACE inhibitory peptides were identified and characterized in both hydrolysates. Furthermore, the hydrolysates were assessed for bitterness. No significant difference was found between the control (milk with no hydrolysate) and hydrolysate samples at different concentrations (at, below and above the IC50)

Personality Traits and Exercise Motivation Among College Students

Exercise and its benefits have been studied for a long period of time. Something that has gained interest is identifying what influences individuals to exercise, and if personality has any impact. The purpose of this study was to understand if conscientiousness and neuroticism influenced different exercise motivations. Participants (N=97, Males= 39, Females= 58) were recruited from academic and athletic clubs at Winona State University. The participants filled out multiple inventories using paper and pencil. This study is looking in particular at the Big Five Inventory (BFI) and Exercise Motivation Inventory-2 (EMI-2). Bivariate correlation analyses were conducted in order to examine the relationship between variables, using SPSS, version 25. Significant positive correlations were found between conscientiousness and ill health avoidance, positive health, and stress management. A significant negative correlation was found between neuroticism and positive health. The results indicate that those who are higher in conscientiousness engage in healthy exercise habits, while those lower in neuroticism may be exercising to improve health. These findings can be useful in identifying personality traits to help motivate individuals to exercisehttps://openriver.winona.edu/urc2019/1079/thumbnail.jp

Eating Behaviors and Cultural Influences on Appearance: What is the Experience of College Women?

Previous research has shown that people who suffer from eating disorders are likely to engage in unhealthy eating behaviors and cultural pressure toward appearance. The purpose of the present study was to replicate previous studies examining unhealthy eating behaviors and cultural values of appearance among college women. It was hypothesized that approximately 50% of college women at WSU engage in unhealthy eating behaviors and college women who report unhealthy eating habits will also note greater awareness of cultural values toward appearance. Seventy-nine undergraduate women participated in a series of online surveys through Qualtrics. Variables examined for this project came from a demographic questionnaire and the Sociocultural Attitudes Toward Appearances Questionnaire-4-Revised (SATAQ-4R). Participants self-reported a range of BMI scores between 16.5 and 42. An independent samples T-test was conducted in order to identify group differences on unhealthy eating behaviors (some or none) and cultural influences. Approximately 50% of participants reported engaging in at least one unhealthy eating behavior. Women who reported engaging in some unhealthy eating behaviors also reported greater internalization of thinness, general attractiveness, and pressure from the media, compared to those who reported none. Campus efforts to improve health among women may be benefitted by interventions that reduce cultural influences on expectations for appearance.https://openriver.winona.edu/urc2019/1093/thumbnail.jp

Equity of utilisation of cardiovascular care and mental health services in England: a cohort-based cross-sectional study using small-area estimation

Background: A strong policy emphasis on the need to reduce both health inequalities and unmet need in deprived areas has resulted in the substantial redistribution of English NHS funding towards deprived areas. This raises the question of whether or not socioeconomically disadvantaged people continue to be disadvantaged in their access to and utilisation of health care.Objectives: To generate estimates of the prevalence of cardiovascular disease (CVD) and common mental health disorders (CMHDs) at a variety of scales, and to make these available for public use via Public Health England (PHE). To compare these estimates with utilisation of NHS services in England to establish whether inequalities of use relative to need at various stages on the health-care pathway are associated with particular sociodemographic or other factors.Design: Cross-sectional analysis of practice-, primary care trust- and Clinical Commissioning Group-level variations in diagnosis, prescribing and specialist management of CVD and CMHDs relative to the estimated prevalence of those conditions (calculated using small-area estimation).Results: The utilisation of CVD care appears more equitable than the utilisation of care for CMHDs. In contrast to the reviewed literature, we found little evidence of underutilisation of services by older populations. Indeed, younger populations appear to be less likely to access care for some CVD conditions. Nor did deprivation emerge as a consistent predictor of lower use relative to need for either CVD or CMHDs. Ethnicity is a consistent predictor of variations in use relative to need. Rates of primarymanagement are lower than expected in areas with higher percentages of black populations for diabetes, stroke and CMHDs. Areas with higher Asian populations have higher-than-expected rates of diabetes presentation and prescribing and lower-than-expected rates of secondary care for diabetes. For both sets of conditions, there are pronounced geographical variations in use relative to need. For instance, the North East has relatively high levels of use of cardiac care services and rural (shire) areas have low levels of use relative to need. For CMHDs, there appears to be a pronounced ‘London effect’, with the number of people registered by general practitioners as having depression, or being prescribed antidepressants, being much lower in London than expected. A total of 24 CVD and 41 CMHD prevalence estimates have been provided to PHE and will be publicly available at a range of scales, from lower- and middle-layer super output areas through to Clinical Commissioning Groups and local authorities.Conclusions: We found little evidence of socioeconomic inequality in use for CVD and CMHDs relative to underlying need, which suggests that the strong targeting of NHS resources to deprived areas may well have addressed longstanding concerns about unmet need. However, ethnicity has emerged as a significant predictor of inequality, and there are large and unexplained geographical variations in use relative to need for both conditions which undermine the principle of equal access to health care for equal needs. The persistence of ethnic variations and the role of systematic factors (such as rurality) in shaping patterns ofutilisation deserve further investigation, as does the fact that the models were far better at explaining variation in use of CVD than mental health services.Funding: The National Institute for Health Research Health Services and Delivery Research programme

The efficient use of the maternity workforce and the implications for safety and quality in maternity care : a population-based, cross-sectional study

Background: The performance of maternity services is seen as a touchstone of whether or not we are delivering high-quality NHS care. Staffing has been identified in numerous reports as being a critical component of safe, effective, user-centred care. There is little evidence regarding the impact of maternity workforce staffing and skill mix on the safety, quality and cost of maternity care in the UK. Objectives: To understand the relationship between organisational factors, maternity workforce staffing and skill mix, cost and indicators of safe and high-quality care. Design and methods: Data included Hospital Episode Statistics (HES) from 143 NHS trusts in England in 2010–11 (656,969 delivery records), NHS Workforce Statistics, England, 2010–11, Care Quality Commission Maternity Survey of women’s experiences 2010 and NHS reference costs 2010/11. Ten indicators were derived from HES data. They included healthy mother and healthy baby outcomes and mode of birth. Adjustments were made for background characteristics and clinical risk. Data were analysed to examine the influence of organisational factors, staffing and costs using multilevel logistic regression models. A production function analysis examined the relationship between staffing, skill mix and output. Results: Outcomes were largely determined by women’s level of clinical risk [based on National Institute for Health and Care Excellence (NICE) guidance], parity and age. The effects of trust size and trust university status were small. Larger trust size reduced the chance of a healthy mother outcome and also reduced the likelihood of a healthy mother/healthy baby dyad outcome, and increased the chances of other childbirth interventions. Increased investment in staff did not necessarily have an effect on the outcome and experience measures chosen, although there was a higher rate of intact perineum and also of delivery with bodily integrity in trusts with greater levels of midwifery staffing. An analysis of the multiplicative effects of parity and clinical risk with the staffing variables was more revealing. Increasing the number of doctors had the greatest impact on outcomes in higher-risk women and increasing the number of midwives had the greatest impact on outcomes in lower-risk women. Although increased numbers of support workers impacted on reducing childbirth interventions in lower-risk women, they also had a negative impact on the healthy mother/healthy baby dyad outcomes in all women. In terms of maximising the capacity of a trust to deliver babies, midwives and support workers were found to be substitutes for each other, as were consultants and other doctors. However, any substitution between staff groups could impact on the quality of care given. Economically speaking, midwives are best used in combination with consultants and other doctors. Conclusions: Staffing levels have positive and negative effects on some outcomes, and deployment of doctors and midwives where they have most beneficial impact is important. Managers may wish to exercise caution in increasing the number of support workers who care for higher-risk women. There also appear to be limited opportunities for role substitution. Future work: Wide variations in outcomes remain after adjustment for sociodemographic and clinical risk, and organisational factors. Further research is required on what may be influencing unexplained variation such as organisational climate and culture, use of NICE guidelines in practice, variation of models of care within trusts and women’s choices. Funding: The National Institute for Health Research Health Services and Delivery Research programme