3,128 research outputs found
Evidence for inbreeding depression in a species with limited opportunity for maternal effects
It is often assumed that mating with close relatives reduces offspring fitness. In such cases, reduced offspring fitness may arise from inbreeding depression (i.e., genetic effects of elevated homozygosity) or from post-mating maternal investment. This can be due to a reduction in female investment after mating with genetically incompatible males ("differential allocation") or compensation for incompatibility ("reproductive compensation"). Here, we looked at the effects of mating with relatives on offspring fitness in mosquitofish, Gambusia holbrooki. In this species, females are assumed to be nonplacental and to allocate resources to eggs before fertilization, limiting differential allocation. We looked at the effects of mating with a brother or with an unrelated male on brood size, offspring size, gestation period, and early offspring growth. Mating with a relative reduced the number of offspring at birth, but there was no difference in the likelihood of breeding, gestation time, nor in the size or growth of these offspring. We suggest that due to limited potential for maternal effects to influence these traits that any reduction in offspring fitness, or lack thereof, can be explained by inbreeding depression rather than by maternal effects. We highlight the importance of considering the potential role of maternal effects when studying inbreeding depression and encourage further studies in other Poeciliid species with different degrees of placentation to test whether maternal effects mask or amplify any genetic effects of mating with relatives.This work was supported bythe Australian Research Council (DP120100339). R.V.-T. is supported by fellowships from Consejo Nacion-al de Ciencia y Tecnologıa-Mexico and the ResearchSchool of Biology
Group B streptococcal infection and activation of human astrocytes.
BACKGROUND:Streptococcus agalactiae (Group B Streptococcus, GBS) is the leading cause of life-threatening meningitis in human newborns in industrialized countries. Meningitis results from neonatal infection that occurs when GBS leaves the bloodstream (bacteremia), crosses the blood-brain barrier (BBB), and enters the central nervous system (CNS), where the bacteria contact the meninges. Although GBS is known to invade the BBB, subsequent interaction with astrocytes that physically associate with brain endothelium has not been well studied. METHODOLOGY/PRINCIPAL FINDINGS:We hypothesize that human astrocytes play a unique role in GBS infection and contribute to the development of meningitis. To address this, we used a well- characterized human fetal astrocyte cell line, SVG-A, and examined GBS infection in vitro. We observed that all GBS strains of representative clinically dominant serotypes (Ia, Ib, III, and V) were able to adhere to and invade astrocytes. Cellular invasion was dependent on host actin cytoskeleton rearrangements, and was specific to GBS as Streptococcus gordonii failed to enter astrocytes. Analysis of isogenic mutant GBS strains deficient in various cell surface organelles showed that anchored LTA, serine-rich repeat protein (Srr1) and fibronectin binding (SfbA) proteins all contribute to host cell internalization. Wild-type GBS also displayed an ability to persist and survive within an intracellular compartment for at least 12 h following invasion. Moreover, GBS infection resulted in increased astrocyte transcription of interleukin (IL)-1β, IL-6 and VEGF. CONCLUSIONS/SIGNIFICANCE:This study has further characterized the interaction of GBS with human astrocytes, and has identified the importance of specific virulence factors in these interactions. Understanding the role of astrocytes during GBS infection will provide important information regarding BBB disruption and the development of neonatal meningitis
Focusing on the Big Picture: Insights into a Systems Approach to Deep Learning for Satellite Imagery
Deep learning tasks are often complicated and require a variety of components
working together efficiently to perform well. Due to the often large scale of
these tasks, there is a necessity to iterate quickly in order to attempt a
variety of methods and to find and fix bugs. While participating in IARPA's
Functional Map of the World challenge, we identified challenges along the
entire deep learning pipeline and found various solutions to these challenges.
In this paper, we present the performance, engineering, and deep learning
considerations with processing and modeling data, as well as underlying
infrastructure considerations that support large-scale deep learning tasks. We
also discuss insights and observations with regard to satellite imagery and
deep learning for image classification.Comment: Accepted to IEEE Big Data 201
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Transcriptomic Profiles of Monocyte-Derived Macrophages in Response to Escherichia coli is Associated with the Host Genetics.
Reactive Nitrogen Species (RNS) are a group of bactericidal molecules produced by macrophages in response to pathogens in a process called oxidative burst. Nitric oxide (NO-) is a member of RNS produced from arginine by inducible Nitric Oxide Synthase (iNOS) enzyme. The activity of iNOS and production of NO- by macrophages following stimulation is one of the indicators of macrophage polarization towards M1/proinflammatory. Production of NO- by bovine monocyte-derived macrophage (MDM) and mouse peritoneal macrophages has been shown to be strongly associated with host genetic with the heritability of 0.776 in bovine MDM and 0.8 in mouse peritoneal macrophages. However, the mechanism of genetic regulation of macrophage response has remained less explored. In the current study, the transcriptome of bovine MDMs was compared between two extreme phenotypes that had been classified as high and low responder based on NO- production. The results showed that 179 and 392 genes were differentially expressed (DE) between high and low responder groups at 3 and 18 hours after exposure to Escherichia coli, respectively. A set of 11 Transcription Factors (TFs) (STAT1, IRF7, SPI1, STAT4, IRF1, HIF1A, FOXO3, REL, NFAT5, HIC1, and IRF4) at 3 hours and a set of 13 TFs (STAT1, IRF1, HIF1A, STAT4, ATF4, TP63, EGR1, CDKN2A, RBL1, E2F1, PRDM1, GATA3, and IRF4) at 18 hours after exposure to E. coli were identified to be differentially regulated between the high and low responder phenotypes. These TFs were found to be divided into two clusters of inflammatory- and hypoxia-related TFs. Functional analysis revealed that some key canonical pathways such as phagocytosis, chemotaxis, antigen presentation, and cell-to-cell signalling are enriched among the over-expressed genes by high responder phenotype. Based on the results of this study, it was inferred that the functional characteristics of bovine MDMs are associated with NO-based classification. Since NO- production is strongly associated with host genetics, this study for the first time shows the distinct proinflammatory profiles of macrophages are controlled by the natural genetic polymorphism in an outbred population. In addition, the results suggest that genetics can be considered as a new dimension in the current model of macrophage polarization which is currently described by the combination of stimulants, only
Inbreeding depression does not increase after exposure to a stressful environment: a test using compensatory growth
BACKGROUND:
Inbreeding is often associated with a decrease in offspring fitness (‘inbreeding depression’). Moreover, it is generally assumed that the negative effects of inbreeding are exacerbated in stressful environments. This G × E interaction has been explored in many taxa under different environmental conditions. These studies usually manipulate environmental conditions either in adulthood or throughout an individual’s entire life. Far fewer studies have tested how stressful environments only experienced during development subsequently influence the effects of inbreeding on adult traits.
RESULTS:
We experimentally manipulated the diet (control versus low food) of inbred and outbred juvenile Eastern mosquitofish (Gambusia holbrooki) for three weeks (days 7-28) to test whether experiencing a presumably stressful environment early in life influences their subsequent growth and adult phenotypes. The control diet was a standard laboratory food regime, while fish on the low food diet received less than 25 % of this amount of food. Unexpectedly, despite a large sample size (237 families, 908 offspring) and a quantified 23 % reduction in genome-wide heterozygosity in inbred offspring from matings between full-siblings (f = 0.25), neither inbreeding nor its interaction with early diet affected growth trajectories, juvenile survival or adult size. Individuals did not mitigate a poor start in life by showing ‘compensatory growth’ (i.e. faster growth once the low food treatment ended), but they showed ‘catch-up growth’ by delaying maturation. There was, however, no effect of inbreeding on the extent of catch-up growth.
CONCLUSIONS:
There were no detectable effects of inbreeding on growth or adult size, even on a low food diet that should elevate inbreeding depression. Thus, the long-term costs of inbreeding due to lower male reproductive success we have shown in another study appear to be unrelated to inbreeding depression for adult male size or the growth rates that are reported in the current study.This work was supported by the Australian Research Council (DP160100285).
R.V.-T. is supported by fellowships from Consejo Nacional de Ciencia y
Tecnología-México and the Research School of Biology
Defects and boundary layers in non-Euclidean plates
We investigate the behavior of non-Euclidean plates with constant negative
Gaussian curvature using the F\"oppl-von K\'arm\'an reduced theory of
elasticity. Motivated by recent experimental results, we focus on annuli with a
periodic profile. We prove rigorous upper and lower bounds for the elastic
energy that scales like the thickness squared. In particular we show that are
only two types of global minimizers -- deformations that remain flat and saddle
shaped deformations with isolated regions of stretching near the edge of the
annulus. We also show that there exist local minimizers with a periodic profile
that have additional boundary layers near their lines of inflection. These
additional boundary layers are a new phenomenon in thin elastic sheets and are
necessary to regularize jump discontinuities in the azimuthal curvature across
lines of inflection. We rigorously derive scaling laws for the width of these
boundary layers as a function of the thickness of the sheet
HONGOS MICORRÍZICOS ARBUSCULARES EN EL CRECIMIENTO DE CAFÉ (Coffea arabica L.) VARIEDADES GARNICA, CATIMOR, CATURRA Y CATUAÍ
Arbuscular mycorrhizal fungi can increase yield, improving growth and the vegetative development of coffee plants. The introduction of arbuscular mycorrhizas during nursery stay increases the fungalbenefits since the adequate management of mycorrhizal inoculum favors the production of more vigorous coffee seedlings at low cost. The effect of two mycorrhizal inoculants was studied: Zac-19 (formed by tree fungal species) and other monospecific (Rhizophagus aggregatus) in four coffee varieties (Garnica, Catimor, Caturra and Catuaí). The plants were inoculated from seed and remained in the nursery for eleven months. The inoculum Zac-19 was the most effective in promoting plant growth and development. Significant differences were obtained between treatments and increments with respect to the control in height (198%), foliar area (676%), root volume (910%) and dry weight (1,063%). Regarding all coffee varieties, it was observed that R. aggregatus was not as efficient as Zac-19, but significantly increased all variables; except plant height. Catuaí variety did not respond favorably to mycorrhizal inoculation. The plant inoculation with Zac-19 allowed obtaining healthy and vigorous plants for field transplant sooner than in the control plants. The use of this fungal inoculum is recommended for coffee nurseries.Los hongos micorrízicos arbusculares pueden incrementar el rendimiento, mejorar el crecimiento y desarrollo vegetativo de las plantas en café. La introducción en vivero de estos hongos es de suma importancia, debido a que el manejo adecuado del inóculo fúngico favorece la producción de plántulas de cafeto más vigorosas a bajo costo. Se estudió el efecto de dos inóculos micorrízicos: uno Zac-19, formado por tres especies y otro monoespecífico (Rhizophagus aggregatus), en cuatro variedades de café (Garnica, Catimor, Caturra y Catuaí). Las plantas permanecieron en vivero once meses, se inoculó desde semilla para evaluar su efecto en las plantas. El inóculo Zac-19 fue más eficiente en promover el crecimiento y desarrollo de las plantas. Se obtuvieron diferencias significativas entre tratamientos e incrementos con respecto al testigo en altura (198%), área foliar (676%), volumen de la raíz (910%) y peso seco (1,063%). Al considerar todas las variedades de café, se observó que R. aggregatus no fue tan eficiente como Zac-19 pero, mostró incrementos significativos con respecto al tratamiento testigo en todas las variables, excepto en altura. La variedad Catuaí no respondió favorablemente a la inoculación micorrízica. Se recomienda la inoculación en el vivero de las plantas de café con Zac-19 y realizar comparación de costos y el tiempo necesario para que plantas inoculadas y las testigo se trasplanten a campo
EFECTO DE LA MICORRIZA ARBUSCULAR EN PLANTAS DE CAFÉ (Coffea arabica L.) INFECTADAS POR EL NEMATODO DE LA CORCHOSIS DE LA RAÍZ
Arbuscular mycorrhizae may reduce the susceptibility and damagescaused by pathogens. Coffee is a crop that has a high degree ofmycotrophy and it has been proven that mycorrhizas improve plantdevelopment. The purpose of this study was to compare the effectof plants inoculated with consortia of mycorrhizal fungi and theirinteraction with phytopathogenic nematodes. Seven consortia ofnative coffee plantation mycorrhizae fungi were isolated, and theinteraction of the different inoculums was compared with the rootknotnematode that causes coffee corky-root disease, also calledcorchosis. For this purpose, coffee plants were inoculated with theseven consortia of AMF and after seven months they were inoculatedwith 1500 juveniles and eggs; the following were defined: dry weight,root volume, leaf area and number of leaves, extraradical mycelium,chlorophyll quantity, percentage of colonization, number of spores,and population of nematodes. The root system volume of plantsinoculated with the pathogen decreased by up to 70.93%; however, theplants observed were apparently healthy and vigorous in the aerial part,which leads us to assume that although the nematode penetrates theroot and damages it, the fungal hyphae allow the passage of nutrientsand therefore the plant attains its development.La micorriza arbuscular puede reducir la susceptibilidad y daños causados por patógenos. El café es un cultivo que presentaun alto grado de micotrofía y ha sido comprobado que la micorriza mejora el desarrollo de la planta. En este trabajose comparó el efecto de plantas inoculadas con consorcios de hongos micorrízicos y su interacción con nematodosfitopatógenos. Se aislaron siete consorcios de hongos micorrízicos nativos de cafetales y se comparó la interacción delos diferentes inóculos con el nematodo causante de la “corchosis”. Para ello se sembraron plantas de café inoculadascon los siete consorcios de HMA y después de siete meses se inocularon con 1500 juveniles y huevecillos y se determinóel peso seco, volumen radical, área foliar y número de hojas, micelio extraradical, cantidad de clorofila, porcentaje decolonización micorrízica, número de esporas y la población de nematodos. El volumen radical de las plantas inoculadascon el patógeno disminuyó hasta en un 70.93%, sin embargo, las plantas se observaban aparentemente sanas y vigorosasen la parte área, lo que nos hace suponer que aunque el nematodo penetra en la raíz y la daña, las hifas del hongospermiten el paso de nutrimentos y así la planta logra su desarrollo
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