Vineyard and winery indicators of 'Shiraz' must fermentation behaviour

Nitrogen supply and rootstock have important consequences for the composition and quantity of nitrogenous compounds in the must, both of which impact on fermentation rate and wine quality. In the Sunraysia district (SE Australia), musts prepared from 'Shiraz' grapes from vines grafted onto three rootstocks and supplied with five different nitrogen (N) regimes were fermented to dryness. Leaf N at flowering and veraison, and berry and juice total N at harvest was influenced by N supply, but the juice total assimilable amino N pool was less sensitive. Consumption rate of soluble solids during fermentation was strongly and positively linearly related to %N in the petioles at veraison. The relationship described could be the basis of a tool to provide oenologists with timely data before harvest and receival on likely fermentation behaviour of specific parcels of grapes, and provide viticulturalists with another recognisable developmental stage to assess the efficacy of vineyard N management strategies within a season.

Simulating Focused Ultrasound Transducers using Discrete Sources on Regular Cartesian Grids

Accurately representing the behaviour of acoustic sources is an important part of ultrasound simulation. This is particularly challenging in ultrasound therapy where multielement arrays are often used. Typically, sources are defined as a boundary condition over a 2D plane within the computational model. However, this approach can become difficult to apply to arrays with multiple elements distributed over a non-planar surface. In this work, a grid-based discrete source model for single and multi-element bowl-shaped transducers is developed to model the source geometry explicitly within a regular Cartesian grid. For each element, the source model is defined as a symmetric, simply-connected surface with a single grid point thickness. Simulations using the source model with the opensource k-Wave toolbox are validated using the Rayleigh integral, O'Neil's solution, and experimental measurements of a focused bowl transducer under both quasi continuous wave and pulsed excitation. Close agreement is shown between the discrete bowl model and the axial pressure predicted by O'Neil's solution for a uniform curved radiator, even at very low grid resolutions. Excellent agreement is also shown between the discrete bowl model and experimental measurements. To accurately reproduce the near-field pressure measured experimentally, it is necessary to derive the drive signal at each grid point of the bowl model directly using holography. However, good agreement is also obtained in the focal region using uniformly radiating monopole sources distributed over the bowl surface. This allows the response of multi-element transducers to be modelled, even where measurement of an input plane is not possible

Experimental validation of k-Wave: Nonlinear wave propagation in layered, absorbing fluid media

Models of ultrasound propagation in biologically relevant media have applications in planning and verification of ultrasound therapies and computational dosimetry. To be effective, the models must be able to accurately predict both the spatial distribution and amplitude of the acoustic pressure. This requires that the models are validated in absolute terms, which for arbitrarily heterogeneous media should be performed by comparison with measurements of the acoustic field. In this study, simulations performed using the open-source k-Wave acoustics toolbox, with a measurement-based source definition, were quantitatively validated against measurements of acoustic pressure in water and layered absorbing fluid media. In water, the measured and simulated spatial peak pressures agreed to within 3% under linear conditions and 7% under non-linear conditions. After propagation through a planar or wedge shaped glycerol-filled phantom, the difference in spatial peak pressure was 8.5% and 10.7%, respectively. These differences are within or close to the expected uncertainty of the acoustic pressure measurement. The -6 dB width and length of the focus agreed to within 4% in all cases, and the focal positions were within 0.7 mm for the planar phantom and 1.2 mm for the wedge shaped phantom. These results demonstrate that when the acoustic medium properties and geometry are well known, accurate quantitative predictions of the acoustic field can be made using k-Wave

Nonlinear 3-D simulation of high-intensity focused ultrasound therapy in the Kidney

Kidney cancer is a severe disease which can be treated non-invasively using high-intensity focused ultrasound (HIFU) therapy. However, tissue in front of the transducer and the deep location of kidney can cause significant losses to the efficiency of the treatment. The effect of attenuation, refraction and reflection due to different tissue types on HIFU therapy of the kidney was studied using a nonlinear ultrasound simulation model. The geometry of the tissue was derived from a computed tomography (CT) dataset of a patient which had been segmented for water, bone, soft tissue, fat and kidney. The combined effect of inhomogeneous attenuation and soundspeed was found to result in an 11.0 dB drop in spatial peak-temporal average (SPTA) intensity in the kidney compared to pure water. The simulation without refraction effects showed a 6.3 dB decrease indicating that both attenuation and refraction contribute to the loss in focal intensity. The losses due to reflections at soft tissue interfaces were less than 0.1 dB. Focal point shifting due to refraction effects resulted in -1.3, 2.6 and 1.3 mm displacements in x-, y- and z-directions respectively. Furthermore, focal point splitting into several smaller subvolumes was observed. The total volume of the secondary focal points was approximately 46% of the largest primary focal point. This could potentially lead to undesired heating outside the target location and longer therapy times

Test materials for characterising heating from HIFU devices using photoacoustic thermometry

High intensity focused ultrasound (HIFU) is a non-invasive thermal therapy during which a focused ultrasound beam is used to destroy cells within a confined volume of tissue. Due to its increased use and advancements in treatment delivery, various numerical models are being developed for use in treatment planning software. In order to validate these models, as well as to perform routine quality checks and transducer characterisation, a temperature monitoring technique capable of accurately mapping the temperature rise induced is necessary. Photoacoustic thermometry is a rapidly emerging technique for non-invasive temperature monitoring, where the temperature dependence of the Gruneisen parameter leads to changes in the recorded photoacoustic signal amplitude with temperature. In order to use this technique to assess heating induced by HIFU in a metrology setting, a suitable test material must first be selected that exhibits an increase in the generated photoacoustic signal with temperature. In this study, the temperature dependence of the photoacoustic conversion efficiency (μaΓ) of several tissue-mimicking materials was measured for temperatures between 22 °C and 50 °C. Materials included were agar-based phantoms, copolymer-in-oil, gel wax, PVA cryogels, PVCP and silicone. This information provided a basis for the development of a volumetric phantom, which was sonicated in a proof-of-concept integrated photoacoustic thermometry system for monitoring of HIFU-induced heating. The results show the suitability of agar-based phantoms and photoacoustic thermometry to image the 3D heat distribution generated by a HIFU transducer

Experimental assessment of skull aberration and transmission loss at 270 kHz for focused ultrasound stimulation of the primary visual cortex

Transcranial focused ultrasound is a rapidly emerging method for non-invasive neuromodulation and stimulation. However, the skull causes a significant acoustic barrier and can reduce the focal intensity and alter the position and shape of the focus compared to free-field. In this study, the insertion loss and focal distortion due to the skull bone were quantified using three ex vivo human skulls and a focused ultrasound transducer operating at 270 kHz targeted on the approximate positions of the left and right primary visual cortex. Compared to free-field, the average insertion loss was -9.8 dB (± 2.2 dB), while the average focal shift was 1.7 mm (± 0.56 mm) in the lateral direction and 2.8 mm (±4.2 mm) in the axial direction. Overall, the acoustic aberrations were small compared to the size of the focal volume, meaning effective stimulation at this frequency can likely be achieved without patient-specific targeting. However, the insertion loss was significant and should be considered when selecting the target focal intensity for human studies

Quantifying the effects of standing waves within the skull for ultrasound mediated opening of the blood-brain-barrier

Ultrasound mediated opening of the blood-brain barrier (BBB) has been shown to be effective in enhancing the delivery of therapeutic agents to the brain. However, challenges remain in targeting and specificity of BBB opening due to attenuation, aberration and reverberation of transcranial ultrasound fields. In this study, experimental and numerical assessment was performed of standing waves within an ex vivo human skull when delivering ultrasound pulses of varying lengths at 300 kHz using a large aperture focused ultrasound transducer. Simulations showed minimal distortion of the focal region but low amplitude standing waves were established within the skull with bursts of 50 cycles or more. Under the same conditions, the experimental measurements showed small variations in focal pressure which took 300 to 600 µs to stabilise. The pattern of sidelobes and superimposed standing waves was generally more complex when the focus was placed closer to the side and base of the skull. This data supports the use of large aperture diameter transducers and short pulse lengths for targeted BBB opening

Measurement of the temperature-dependent speed of sound and change in Gruneisen parameter of tissue-mimicking materials

Knowledge of the temperature dependence of the material properties of tissue-mimicking materials is useful or essential for many applications. This includes photoacoustic thermometry where the temperature dependence of the Grüneisen parameter of tissues leads to changes in the recorded photoacoustic signal amplitude with temperature. Here, a setup is described that can measure the temperature dependence of the speed of sound and photoacoustic conversion efficiency (μ a Γ) of tissue-mimicking materials. Agar-based phantoms, copolymer-in-oil, gel wax, PVCP, silicone and water were characterised in the newly developed setup for temperatures between 22°C and 50°C. This information provides a valuable resource for material characterisation and future development of tissue-mimicking materials

Investigation of the repeatability and reproducibility of hydrophone measurements of medical ultrasound fields

Accurate measurements of acoustic pressure are required for characterisation of ultrasonic transducers and for experimental validation of models of ultrasound propagation. Errors in measured pressure can arise from a variety of sources, including variations in the properties of the source and measurement equipment, calibration uncertainty, and processing of measured data. In this study, the repeatability of measurements made with four probe and membrane hydrophones was examined. The pressures measured by these hydrophones in three different ultrasound fields, with both linear and nonlinear, pulsed and steady state driving conditions, were compared to assess the reproducibility of measurements. The coefficient of variation of the focal peak positive pressure was less than 2% for all hydrophones across five repeated measurements. When comparing hydrophones, pressures measured in a spherically focused 1.1 MHz field were within 7% for all except 1 case, and within 10% for a broadband 5 MHz pulse from a diagnostic linear array. Larger differences of up to 55% were observed between measurements of a tightly focused 3.3 MHz field, which were reduced for some hydrophones by the application of spatial averaging corrections. Overall, the major source of these differences was spatial averaging and uncertainty in the complex frequency response of the hydrophones