Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats

Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.U našoj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaštitne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naših rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova.Projekat ministarstva br. 143035

Цијанобактерије и алге из биофилма са улазне зоне Петничке пећине

The importance of biofilms in caves, the diversity of microorganisms in them, their mutual relationship and relationship with the substratum are among the advancing research topics in microbial biospeleology. This research is making contribution to the knowledge about biofilms at cave entrances and phototrophic communities in them. In that manner, biofilms from the entrance zone of the Petnica Cave were examined. Light microscopy showed that cyanobacteria were exclusively dominant phototrophs (34 taxa out of 39 total taxa recorded) with coccoid forms prevailing (28 taxa); simple trichal forms were present to a lesser extent, while heterocytous ones were completely absent. Genera Gloeocapsa, Chroococcus,Gloeothece and Leptolyngbya were the most diverse. Four green algal genera characteristic for aerophytic habitats (Apatococcus, Desmococcus, Haematococcus and Trentepohlia) were also recorded, while Bacillariophyta were observed sporadically. Three groups of sampling sites were distinguished based on recorded taxa, their richness and similarity, using non-metric multidimensional scaling (NMDS). Quantitative biofilm characteristics were also assessed – the content of chlorophyll a (Chl a) was determined, as well as the contents of water, organic and inorganic matter. Chl a had a significant positive correlation with the content of organic matter (r=0.904, P=0.013).Значај биофилмова у пећинама, разноврсност микроорганизама у њима, њихов међусобни однос, као и однос са супстратом, спадају у водеће теме истраживања у пољу микробијалне биоспелеологије. Ово истраживање доприноси општем познавању биофилмова на улазима пећина, као и познавању фототрофних заједница које се налазе у њиховом саставу. Имајући то у виду, испитивани су биофилмови са улазне зоне Петничке пећине. Светлосна микроскопија је показала да су цијанобактерије искључиво доминантни фототрофи (забележено је 34 од укупно 39 таксона), са изузетном доминацијом кокоидних форми (28 таксона); трихалне форме (хомоцитни представници) су у биофилму заступљене у мањој мери, док су хетероцитне потпуно одсутне. У родовима Gloeocapsa, Chroococcus, Gloeothece и Leptolyngbya документован је највећи број таксона. Четири рода зелених алги која су иначе карактеристична за аерофитска станишта (Apatococcus, Desmococcus, Haematococcus и Trentepohlia) су такође забележена, док су представници раздела Bacillariophyta нађени спорадично. Статистичка анализа NMDS је показала да су се према сличности, односно на основу таксона који су идентификовани, одвојиле три групе тачака узорковања. Kвантитативне карактеристике биофилма су такође процењене – одређен је садржај хлорофила а (Chl a), као и садржај воде, органске и неорганске материје. Значајнa позитивна корелација (r=0.904, P=0.013) уочена је између садржаја органске материје и Chl a

Substrate type selection in diatom based lake water quality assessment

Various studies report contrasting results on the substrate-type effect on diatom community composition, but the particularly important question is whether or not it affects diatom-based assessments of water quality. We investigated whether the substrate type is a significant predictor of the diatom community composition and if it affects lake water quality assessment based on diatom indices. This study took place in Sava Lake (Serbia). We used glass, ceramic, willow and yew tree tiles as artificial substrates for periphyton development, and pebbles from the lake littoral as natural substrate. Results revealed differences in both the diatom community composition and diatom indices values related to the substrates. A distinction was recognized between natural, artificial wooden, and artificial inert substrates. However, the final lake quality assessment based on diatom indices was more or less similar in all substrate types in our study, and depended on value ranges associated with water quality classification and on diatom index choices. Artificial substrates in our study did show potential as an alternative for natural substrate, but further studies are required, particularly in various types of lentic ecosystems to confirm our findings and support artificial substrate employment in lake water quality assessment

Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.U našoj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaštitne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naših rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova.Projekat ministarstva br. 143035

Novi i značajni podaci o biljkama, algama i gljivama iz JI Evrope i susednih regiona, 16

This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: diatoms Discostella asterocostata and Stephanodiscus hantzschii f. tenuis, red alga Bangia atropurpúrea, green alga Ulvapilifera, saprotro-phic fungi Didymella vitalbina and Phragmotrichum rivoclarinum, mosses Buxbau-mia aphylla, Sphagnum divinum, and Tortella fasciculata, monocots Anacamptis x nicodemi, Epipactis palustris, Epipogium aphyllum, and Gymnadenia frivaldii and dicots Androsace lactea, Drosera rotundifolia, Potentilla montenegrina, and Tozzia alpina subsp. carpathica are given within SE Europe and adjacent regions.U radu su prikazani novi i značajni podaci sa područja JI Evrope i susednih regiona o sledećim taksonima: dijatomejskim algama Discostella asterocostata i Stephanodiscus hantzschii f. tenuis, crvenoj algi Bangia atropurpurea, zelenoj algi Ulva pilifera, saprofitskim gljivama Didymella vitalbina i Phragmotrichum rivoclarinum, mahovinama Buxbaumia aphylla, Sphagnum divinum i Tortella fascicu- lata, monokotilama Anacamptis × nicodemi, Epipactis palustris, Epipogium aphyllum i Gymnadenia frivaldii i dikotilama Androsace lactea, Drosera rotundifolia, Potentilla montenegrina i Tozzia alpina subsp. carpathica

Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

Interactions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it.<0.001) by Ox-gal-1 at 1 µg/ml. Both sets of results confirmed involvement of gal-1 in trophoblast invasion. Galectin profile of isolated cytotrophoblast and HTR-8/SVneo cells was established using RT-PCR and real-time PCR and found to consist of gal-1, gal-3 and gal-8 for both cell types. Only gal-1 was located at the trophoblast cell membrane, as determined by FACS analysis, which is consistent with the results of the functional tests.These findings qualify gal-1 as a member of human trophoblast cell invasion machinery

New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 15

This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: saprotrophic fungus Geastrum morganii, Guignardia istriaca and Hypoxylon howeanum, mycorrhizal fungus Amanita friabilis and Suillus americanus, xanthophyte Vaucheria frigida, stonewort Chara hispida, liverwort Calypogeia integristipula and Ricciocarpus natans, moss Campylopus introflexus, Dicranum transsylvanicum, Tortella pseudofragilis and Trematodon ambiguus, fern Ophioglossum vulgatum subsp. vulgatum, monocots Epipactis exilis, Epipactis purpurata and Epipogium aphyllum and dicots Callitriche cophocarpa, Cornus sanguinea subsp. hungarica and Viscum album subsp. austriacum are given within SE Europe and adjacent regions

Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q(10) and Vitamin E

The aim of this study was to investigate the protective role of coenzyme Q(10) (CoQ(10), 20 mg/kg) and Vitamin E (Vit E, 20IU/kg) alone or in combination against cadmium (Cd, 0.4 mg/kg) induced lipid peroxidation and changes in antioxidant defense system in the rat testes. The obtained results showed that Cd increased lipid peroxidation in the testes, suggesting that Cd-induced oxidative stress, while CoQ(10) and Vit E treatment reversed this change to control values. Acute intoxication with Cd was followed by significantly decreased activity of antioxidant enzymes (SOD, CAT, GSH-Px, GR and GST). Vitamins C and E concentrations also significantly declined in Cd-exposed rat testes. Treatment with CoQ(10), and Vit E reversed Cd-induced alterations of antioxidant defense system and significantly prevented Cd-induced testes damage. These results suggest that both CoQic, and Vit E function as a potent antioxidant in protection of rats testes against the oxidative stress induced by Cd. (C) 2009 Elsevier Inc. All rights reserved.Ministry of Science and Technological Development of Republic of Serbia [143035B

DOI:10.2298/ABS1001075T EFFECTS OF CISPLATIN ON LIPID PEROXIDATION AND THE GLUTATHIONE REDOX STATUS IN THE LIVER OF MALE RATS: THE PROTECTIVE ROLE OF SELENIUM

Abstract –The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats