Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus

The study was co-funded by grants RTA2011-00132-C2 (M.J.A.), AGL2014-56675-R (M.J.A.), AGL2017-82452-C2 (A.Be., M.J.A.), and PID2021-124599OB-I00 (A.Be.) from the Spanish Ministry of Science and Innovation/the Spanish Research Agency, by grant 51917 (M.J.A.) from the Generalitat Valenciana, as well as the European Regional Development Fund (ERDF), MCIN/AEI/10.13039/501100011033, and ERDF “A way of making Europe”. J.E. was supported by a JAE Intro CSIC grant (JAEINT_19_00566).Supplementary Materials: The supporting information can be downloaded at: https://www.mdpi. com/article/10.3390/ijms242115759/s1The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the salt-tolerant Cleopatra mandarin (Citrus reshni) and the disease-resistant donor Poncirus trifoliata were performed with the aim to identify repeatable QTLs that regulate leaf Cl− (and/or Na+) exclusion across independent experiments in citrus, as well as potential candidate genes involved. A repeatable QTL controlling leaf Cl− was detected in chromosome 6 (LCl-6), where 23 potential candidate genes coding for transporters were identified using the C. clementina genome as reference. Transcriptomic analysis revealed two important candidate genes coding for a member of the nitrate transporter 1/peptide transporter family (NPF5.9) and a major facilitator superfamily (MFS) protein. Cell wall biosynthesis- and secondary metabolism-related processes appeared to play a significant role in differential gene expression in LCl-6. Six likely gene candidates were mapped in LCl-6, showing conserved synteny in C. reshni. In conclusion, markers to select beneficial Cleopatra mandarin alleles of likely candidate genes in LCl-6 to improve salt tolerance in citrus rootstock breeding programs are provided.Spanish Ministry of Science and Innovation/Spanish Research Agency RTA2011-00132-C2, AGL2014-56675-R, AGL2017-82452-C2, PID2021-124599OB-I00Generalitat Valenciana 51917European Regional Development Fund (ERDF)MCIN/AEI/10.13039/501100011033ERDF “A way of making Europe”CSIC grant (JAEINT_19_00566

HKT1;1 and HKT1;2 Na+ Transporters from Solanum galapagense Play Different Roles in the Plant Na+ Distribution under Salinity

The study was funded by the Spanish Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion, grants AGL2017-82452-C2-1R (A.B.) and AGL2017-82452-C2-2R (M.J.A) both from the, MCIN/AEI/10.13039/501100011033, the European Union (H2020 SFS-28-2020), contract number 101000716 (HARNESSTOM), and FEDER "Una manera de hacer Europa". J.E. was supported by a JAE Intro-CSIC grant, JAEINT_19_00566.Salt tolerance is a target trait in plant science and tomato breeding programs. Wild tomato accessions have been often explored for this purpose. Since shoot Na+/K+ is a key component of salt tolerance, RNAi-mediated knockdown isogenic lines obtained for Solanum galapagense alleles encoding both class I Na+ transporters HKT1;1 and HKT1;2 were used to investigate the silencing effects on the Na and K contents of the xylem sap, and source and sink organs of the scion, and their contribution to salt tolerance in all 16 rootstock/scion combinations of non-silenced and silenced lines, under two salinity treatments. The results show that SgHKT1;1 is operating differently from SgHKT1;2 regarding Na circulation in the tomato vascular system under salinity. A model was built to show that using silenced SgHKT1;1 line as rootstock would improve salt tolerance and fruit quality of varieties carrying the wild type SgHKT1;2 allele. Moreover, this increasing effect on both yield and fruit soluble solids content of silencing SgHKT1;1 could explain that a low expressing HKT1;1 variant was fixed in S. lycopersicum during domestication, and the paradox of increasing agronomic salt tolerance through silencing the HKT1;1 allele from S. galapagense, a salt adapted species.Spanish Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion AGL2017-82452-C2-1R AGL2017-82452-C2-2R MCIN/AEI/10.13039/501100011033European Commission 101000716FEDER "Una manera de hacer Europa" JAEINT_19_0056

Caracterización génica, proteínica y funcional de tiorredoxinas de guisante (pisum sativum)

Tesis Univ. Granada. Instituto de Biotecnología. Leída el 7 de julio de 200

TV - Arquitectura y Medio Ambiente - AR309 - 202102

Descripción: El curso TV - Arquitectura y Medio Ambiente, es un taller que toma como énfasis el entorno natural, la sostenibilidad y los medios pasivos de climatización y adecuación a las posibles inclemencias naturales. Procurando al mismo tiempo, defender al medio natural del posible impacto generado por la edificación, comprendiendo a ambos como un todo integrado. Propósito: l propósito del curso es el de permitir al futuro arquitecto poder abordar un proyecto arquitectónico en diversas escalas, desde lo macro (con la comprensión del lugar y de las condicionantes del medio geográfico) hasta lo micro (con la correcta utilización de materiales y criterios constructivos), desarrollando una sensibilidad hacia el medio ambiente. La asignatura del Taller V contribuye al desarrollo de la competencia general: Pensamiento Innovador, y las competencias específicas: Diseño fundamentado (que corresponde a los criterios NAAB: PC2, PC3, PC5 y SC5) y, Técnica y construcción (que corresponde a los criterios NAAB: SC1, SC4 y SC6), todas estas en el nivel 2. Este curso tiene como requisito haber aprobado el Taller AR308 TIV - Arquitectura y Funcionalidad