PCR real time assays for the early detection of BKV-DNA in immunocompromised patients

Testing for viral BKV-DNA in urine is a non-invasive early detection and monitoring tool in the diagnostic of BKVrelated pathologies: quantitative analysis by Real-Time PCR can provide useful information in addition to cytologic analysis, although our study suggests that high BKV viruria is not necessarily associated with kidney or bladder damage

INCB84344-201: Ponatinib and steroids in frontline therapy for unfit patients with Ph+ acute lymphoblastic leukemia

Tyrosine kinase inhibitors have improved survival for patients with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL). However, prognosis for old or unfit patients remains poor. In the INCB84344-201 (formerly GIMEMA LAL 1811) prospective, multicenter, phase 2 trial, we tested the efficacy and safety of ponatinib plus prednisone in newly diagnosed patients with Ph+ ALL 6560 years, or unfit for intensive chemotherapy and stem cell transplantation. Forty-four patients received oral ponatinib 45 mg/d for 48 weeks (core phase), with prednisone tapered to 60 mg/m2/d from days-14-29. Prophylactic intrathecal chemotherapy was administered monthly. Median age was 66.5 years (range, 26-85). The primary endpoint (complete hematologic response [CHR] at 24 weeks) was reached in 38/44 patients (86.4%); complete molecular response (CMR) in 18/44 patients (40.9%) at 24 weeks. 61.4% of patients completed the core phase. As of 24 April 2020, median event-free survival was 14.31 months (95% CI 9.30-22.31). Median overall survival and duration of CHR were not reached; median duration of CMR was 11.6 months. Most common treatment-emergent adverse events (TEAEs) were rash (36.4%), asthenia (22.7%), alanine transaminase increase (15.9%), erythema (15.9%), and \u3b3-glutamyltransferase increase (15.9%). Cardiac and vascular TEAEs occurred in 29.5% (grade 653, 18.2%) and 27.3% (grade 653, 15.9%), respectively. Dose reductions, interruptions, and discontinuations due to TEAEs occurred in 43.2%, 43.2%, and 27.3% of patients, respectively; 5 patients had fatal TEAEs. Ponatinib and prednisone showed efficacy in unfit patients with Ph+ ALL; however, a lower ponatinib dose may be more appropriate in this population. This trial was registered at www.clinicaltrials.gov as #NCT01641107

Stimulatory autoantibodies to PDGF receptor in patients with extensivechronic graft-versus-host disease

Extensive chronic graft-versus-host disease (ecGVHD) is characterized by fibrosis similar to that of patients with systemic sclerosis (scleroderma). Since stimulatory autoantibodies against the platelet-derived growth factor (PDGF) receptor (PDGFR) have been found in patients with scleroderma and are responsible for the activation of skin fibroblasts, we tested the hypothesis that these autoantibodies are also present in patients affected by ecGVHD. Serum from 39 patients subjected to allogeneic stem cell transplantation for hematologic malignancies (22 with ecGVHD and 17 without cGVHD) and 20 healthy controls was assayed for the presence of stimulatory autoantibodies to the PDGFR by incubating purified IgG with mouse-embryo fibroblasts lacking PDGFR or chains or with the same cells expressing PDGFR . Stimulatory antibodies to the PDGFR were found selectively in all patients with ecGVHD but in none of the patients without cGVHD. Higher levels were detected in patients with generalized skin involvement and/or lung fibrosis. Antibodies recognized native PDGFR, induced tyrosine phosphorylation, accumulation of reactive oxygen species (ROS), and stimulated type 1 collagen gene expression through the Ha-Ras-ERK1/2-ROS signaling pathway. The biologic activity of these autoantibodies suggests a role in the development of fibrosis and argues for a common pathogenetic trait in ecGVDH and scleroderma phenotypes

Low-dose Gemtuzumab-Ozogamicin as post-consolidation therapy in elderly patients with acute myeloid leukaemia: a pilot study.

The incidence of acute myeloid leukemia (AML) increases with advancing age, and in older patients the chance of cure has not substantially improved recently. In the elderly the incidence of secondary AML is high, and is often associated with both high- risk cytogenetic abnormalities and expression of the multidrug resistance protein (MDR1) and p-glycoprotein (p-gp), both of which are associated with poor outcomes (Appelbaum et al, 2006). Gemtuzumab-Ozogamicin (GO) is a humanized anti-CD33 monoclonal antibody conjugated to Calicheamicin that is rapidly internalized after binding to CD33. GO seems to be more selective than conventional chemotherapy, as CD33 is expressed on AML cells but not in normal haematopoietic stem cells (SCs) or in non-haematopoietic tissues (Sievers et al, 2001). In a series of phase II studies including 142 patients with AML in first relapse, GO monotherapy was associated with a 30% overall complete remission (CR) rate, including a 26% rate in patients over 60 years of age (Sievers et al, 2001; Larson et al, 2002). These results led to US Food and Drug Administration approval of GO for the treatment of patients over 60 years with relapsed AML (Bross et al, 2001). As a consequence of these results, there is interest in extending the use of GO to a frontline treatment for AML in combination with conventional chemotherapy. Little is known about the usefulness of GO as consolidation and/or maintenance therapy, and no data on the topic have been published to date. In particular, there are no data concerning the safety and efficacy of GO in the setting of post-consolidation therapy in AML patients except for a short report concerning the effects after autologous stem cell transplantation (ASCT) (Cascavilla et al, 2008). GO mono- therapy has typically been administered as a 2-h infusion at a dose of 9 mg/m2 on days 1 and 15 of treatment, but the administration of fractionated doses has recently been reported to have a better safety profile (Taksin et al, 2007). We evaluated the efficacy of low-dose GO as late consolidation therapy after CR in a subset of fit elderly patients who were enrolled in a prospective study. From June 1999 to December 2007, 125 patients of 60 years of age or older with morphologically-confirmed AML and non-acute promyelocytic leukaemia were observed in our institution. The preliminary results from 42 patients were reported in 2007 (Olivieri et al, 2007). Fit patients, selected according to previously published inclusion criteria (Olivieri et al, 2007), were treated with intensive chemotherapy, followed by SC mobilization and ASCT (Olivieri et al, 2007). Patients who successfully mobilized SCs underwent ASCT, while poor mobilisers received a further consolidation including standard chemotherapy or investigational immunotherapy with GO. Among the initial 125 patients, 79 fulfilled the inclusion criteria; of those, 56 (72Æ1%) achieved CR, and 52 received the first intensive consolidation course followed by G-CSF to collect SC for ASCT. In cases of mobilisation failure, patients were allowed to chose between an experimental approach B J H 8 1 6 8 B Dispatch: 8.3.10 Journal: BJH CE: Varun Kumar Journal Name Manuscript No. Author Received: No. of pages: 3 PE: Subhashree Table I. Clinical and biological characteristics of the three groups of patients receiving consolidation with GO (A), ASCT (B), Chemo- therapy (CHT) (C) and Allogeneic Transplantation (D). A (%) B (%) C (%) D (%) P Gender Male 8 (62) 8 (42Æ1) 3 (50) 3 (60) N.S. Female 5 (38) 11 (57Æ9) 3 (50) 2 (40) Age (years) Median = 70 (range, 61–76) £70 7 (54) 10 (52Æ6) 5 (83Æ3) 4 (80) N.S. >70 6 (46) 9 (47Æ4) 1 (16Æ7) 1 (20) FAB subtype M0 0 5 (26Æ5) 0 0 N.S. M1 3 (23) 4 (21) 2 (33Æ3) 2 (40) M2 3 (23) 8 (42) 4 (66Æ7) 2 (40) M4 3 (23) 2 (10Æ5) 0 1 (20) M5 3 (23) 0 0 0 M6 1 (8) 0 0 0 M7 0 0 0 0 Leucocytosis (·109/l) WBC <10 8 (61Æ5) 9 (47Æ4) 4 (66Æ7) 4 (80) N.S. WBC 10–50 5 (38Æ5) 6 (31Æ6) 1 (16Æ7) 1 (20) WBC >50 0 4 (21) 1 (16Æ7) 0 Karyotype Poor 2 (15Æ4) 7(36Æ8) 1 (16Æ7) 4 (80) N.S. Intermediate 6 (46Æ2) 8 (42Æ1) 4 (66Æ7) 0 Favourable 2 (15Æ4) 0 1 (16Æ7) 1 (20) NE 3 (15Æ4) 4 (21Æ1) 0 0 Secondary disease* Yes 3 (23) 8 (42) 2 (33Æ3) 1 (20) N.S. No 10 (77) 11 (58) 4 (66Æ7) 4 (80) FAB, French-American-British classification; NE, not evaluated; WBC, white blood cell. *To chemotherapy or Myelodysplastic Syndrome. correspondence ª 2010 Blackwell Publishing Ltd, British Journal of Haematology doi:10.1111/j.1365-2141.2010.08168.x 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 including low-dose GO or a further conventional consolidation course. GO was administered on a compassionate basis, and the costs were charged to our department. Among the 52 patients who received intensive consolidation, two died and seven relapsed; thus, 43 patients were evaluable for post-remission treatment after the SC mobilization attempt. Of those, 19 patients (44%) successfully mobilized SC and received ASCT. Of the 24 that did not mobilise SC, 13 received GO, six patients refused GO and received a second consolidation with chemo- therapy, and five patients received reduced intensity conditioning allogeneic transplant from sibling donors. The current analysis did not include all patients receiving allogeneic transplant because of the poor prognosis of the disease. The disease characteristics of the remaining patients were equally distributed in the three groups, and the data are shown in Table I. All the patients received GO at a dose of 3 mg/m2 three times monthly on an outpatient basis and received common antimicrobial prophylaxis. No patients needed hospitalisation for infections or other major toxicities; the median duration of neutropenia (PMN <0Æ5 · 109/l) after GO was 12 d (range 0–33 d). The main toxicities (World Health Organization grade III–IV) were myelosuppression (n = 9), hypertransam- inasaemia (n = 1) and anaphylaxis (n = 3); no major unex- pected adverse events were observed. With a median follow up of 58 months (range 19–89), a total of 15 patients were alive and in CR: five received ASCT (median follow-up 77 months, range 45-89), nine received GO (median follow-up 38 months, range: 19–75 months), and one, who received chemotherapy, has been followed for 72 months. Two patients receiving GO relapsed and eventually died after 13 and 19 months from CR after the first consolidation. Two more patients relapsed after 15 and 32 months after a second CR after salvage chemother- apy, followed by three doses of GO 3 mg/m2 administered as consolidation therapy. In conclusion, nine of the 13 patients who received GO as late consolidation therapy were alive and in continuous CR (including two patients with secondary AML and two with a complex karyotype). The Landmark survival analysis showed better overall survival (OS) and disease-free survival (DFS) (P = 0Æ017 and 0Æ01 respectively) in the 13 patients that received GO (5-year OS, 60%; 5-year DFS, 67%) compared with patients that received either ASCT (5-year OS and DFS: 26%) or chemotherapy (5-year OS and DFS: 17%) (Fig 1). Our preliminary data support a potential role for low-dose GO in consolidation therapy in elderly patients with AML who are able to achieve CR after intensive induction. Late consolidation with low-dose GO seems to be safe and easily manageable; the myelosuppression was relevant, but generally short. All patients received the 3 GO infusions on an outpatient basis without further readmissions and without fatal events. These preliminary data encourage the use of low-dose GO as late consolidation therapy to eliminate the minimal residual disease (MRD) in older patients with AML. Larger studies are needed for confirmation, possibly including monitoring of MRD during treatment. It also remains to be established if SC collection failure after CR represents an independent favour- able prognostic factor in AML patients, as suggested by some retrospective data (Keating et al, 2003). Antonella Poloni1 Debora Capelli1 Silvia Trappolini1 Benedetta Costantini1 Mauro Montanari1 Guido Gini1 Ilaria Scortechini1 Giorgia Mancini1 Giancarlo Discepoli2 Pietro Leoni1 Attilio Olivieri3 1Dipartimento di Scienze Mediche e Chirurgiche, Sezione di Ematologia, Universita` Politecnica delle Marche and Azienda Ospedaliera Ospedali Riuniti, 2Laboratorio di Citogenetica e Genetica Molecolare, Clinica di Pediatria, Ospedali Riuniti, Ancona, and 3Azienda Ospedaliera San Carlo, Potenza, Italy. E-mail: [email protected] 0 20 40 60 80 100 0 12 24 36 48 60 72 84 96 Cumulative probability (%) Months OS GO ASCT CHT 0 20 40 60 80 100 0 12 24 36 48 60 72 84 96 Cumulative probability (%) Months DFS Fig 1. Comparison of the outcome (OS and DFS) of patients receiving late consolidation with Gemtuzumab-Ozogamicin (GO), autologous stem cell transplantation (ASCT) or chemotherapy (CHT) (log rank test). Correspondence 2 ª 2010 Blackwell Publishing Ltd, British Journal of Haematology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 References Appelbaum, F.R., Gundacker, H., Head, D.R., Slovak, M.L., Willman, C.L., Godwin, J.E., Anderson, J.E. & Petersdorf, S.H. (2006) Age and acute myeloid leukaemia. Blood, 107, 3481–3485. Bross, P.F., Beitz, J., Chen, G., Chen, X.H., Duffy, E., Kieffer, L., Roy, S., Sridhara, R., Rahman, A., Williams, G. & Pazdur, R. (2001) Approval summary: gemtuzumab ozogamicin in relapsed acute myeloid leukemia. Clinical Cancer Research, 7, 1490–1496. Cascavilla, N., D’Arena, G., Greco, M.M., Melillo, L., Merla, E. & Carella, A.M. (2008) Gemtuzumab-Ozogamicin as maintenance therapy after Autologous Stem Cell Transplantation in elderly patients with Acute Myeloid Leukaemia. British Journal of Haematology , 142, 852–853. Keating, S., Suciu, S., de Witte, T., Zittoun, R., Mandelli, F., Belhabri, A., Amadori, S., Fibbe, W., Gallo, E., Fillet, G., Varet, B., Meloni, G., Hagemeijer, A., Fazi, P., Solbu, G., Willemze, R., EORTC Leukemia Group & GIMEMA Leukemia Group. (2003) The stem cell mobilizing capacity of patients with acute myeloid leukemia in complete remission correlates with relapse risk: results of EORTC- GIMEMA AML 10 trial. Leukemia, 17, 60–67. Larson, R.A., Boogaerts, M., Estey, E., Karanes, C., Stadtmauer, E.A., Sievers, E.L., Mineur, P., Bennett, J.M., Berger, M.S., Eten, C.B., Munteanu, M., Loken, M.R., Van Dongen, J.J., Bernstein, I.D., Appelbaum, F.R. & Mylotarg Study Group. (2002) Antibody- targeted chemotherapy of older patients with acute myeloid leukemia in first relapse using Mylotarg (gemtuzumab ozogamicin). Leukemia, 16, 1627–1636. Olivieri, A., Capelli, D., Troiani, D., Poloni, A., Montanari, M., Offidani, M., Discepoli, G. & Leoni, P. (2007) A new intensive induction schedule, including high-dose Idarubicin, high-dose Aracytin and Amifostine, in older AML patients: feasibility and long-term results in 42 patients. Experimental Hematology, 35, 1074–1082. Sievers, E.L., Larson, R.A., Stadtmauer, E.A., Estey, E., Lo¨wenberg, B., Dombret, H., Karanes, C., Theobald, M., Bennett, J.M., Sherman, M.L., Berger, M.S., Eten, C.B., Loken, M.R., Van Dongen, J.J., Bernstein, I.D., Appelbaum, F.R. & Mylotarg Study Group. (2001) Efficacy and safety of gemtuzumab ozogamicin in patients with CD33-positive acute myeloid leukemia in first relapse. Journal of Clinical Oncology, 19, 3244–3254. Taksin, A.L., Legand, O., Raffoux, E., de Revel, T., Thomas, X., Con- tenin, N., Bouabdallah, R., Pautas, C., Turlure, P., Reman, O., Gardin, C., Varet, B., de Botton, S., Pousset, F., Farhat, H., Chevret, S., Dombret, H. & Castaigne, S. (2007) High efficacy and safety profile of fractionated doses of Mylotarg as induction therapy in patients with relapsed acute myeloblastic leukemia: a prospective study of the alfa group. Leukemia, 21, 66–71. Keywords: AML, chemotherapy,

Gemtuzumab-Ozogamicin as Post-Consolidation Therapy In Elderly Patients with Acute Myeloid Leukemia: a Pilot Study

none12Debora, Capelli; Mancini, Giorgia; Chiarucci, Martina; Saraceni, Francesco; Poloni, Antonella; Trappolini, Silvia; Costantini, Benedetta; Montanari, Mauro; Gini, Guido; Scortechini, Ilaria; Leoni, Pietro; Olivieri, AttilioDebora, Capelli; Mancini, Giorgia; Chiarucci, Martina; Saraceni, Francesco; Poloni, Antonella; Trappolini, Silvia; Costantini, Benedetta; Montanari, Mauro; Gini, Guido; Scortechini, Ilaria; Leoni, Pietro; Olivieri, Attili

Telomere length, c-myc and mad-1 expression could represent prognosis markers of myelodysplastic syndrome

tTelomere dysfunction might generate genomic instability leading to the progression of myelodysplasticsyndromes (MDS) into acute myeloid leukemia (AML). We investigated telomere length (TL), telome-rase activity (TA) and hTERT, c-myc, mad1, and p53 expression in the bone marrow of patients withMDS (n = 109), AML (n = 47) and in controls (n = 24). TL was lower in MDS patients than in controls(p < 0.001) and higher in L-MDS (low, intermediate-1 IPSS, p < 0.01) respect H-MDS (high, intermediate-2IPSS, p < 0.01) patients. Mad-1 expression was higher in MDS patients than in controls (p < 0.01), c-mycexpression was highest in AML and in H-MDS patients. Our results show that the telomere dynamicsmight be useful for stratifying patients according to a risk scoring system