Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam.

Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012-2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections

Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam.

BACKGROUND: Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays for ARI in Vietnam. METHODS: Four hundred and forty two nose and throat swabs were collected in viral transport medium, and were tested with Luminex xTAG Respiratory Viral Panel FAST v2. Multiplex RT-PCR and single RT-PCR were used as references.    Results: Overall, viral pathogens were detected in a total count of 270/294 (91.8%, 95% CI 88.1-94.7) by the Luminex among reference assays, whilst 112/6336 (1.8%, 95% CI, 1.4-2.1) of pathogens were detected by the Luminex, but not by reference assays. Frequency of pathogens detected by Luminex and reference assays was 379 and 292, respectively. The diagnostic yield was 66.7% (295/442, 95%CI 62.1-71.1%) for the Luminex assay and 54.1% (239/442, 95% CI, 49.3-58.8%) for reference assays. The Luminex kit had higher yields for all viruses except influenza B virus, respiratory syncytial virus, and human bocavirus. High agreements between both methods [mean (range): 0.91 (0.83-1.00)] were found for 10/15 viral agents. CONCLUSIONS: The Luminex assay is a high throughput multiplex platform for rapid detection of common viral pathogens causing ARI. Although the current high cost may prevent Luminex assays from being widely used, especially in limited resource settings where ARI are felt most, its introduction in clinical diagnostics may help reduce unnecessary use of antibiotic prescription

Climate-induced storminess forces major increases in future storm surge hazard in the South China Sea region

Coastal floods, driven by extreme sea levels, are one of the most dangerous natural hazards. The people at highest risk are those living in low-lying coastal areas exposed to tropical-cyclone-forced storm surges. Here we apply a novel modelling framework to estimate past and/or present and future storm-surge-level and extreme-sea-level probabilities along the coastlines of southern China, Vietnam, Cambodia, Thailand, and Malaysia. A regional hydrodynamic model is configured to simulate 10 000 years of synthetic tropical cyclone activity, representative of a past/present (1980–2017) and high-emission-scenario future (2015–2050) period. Results show that extreme storm surges, and therefore total water levels, will increase substantially in the coming decades, driven by an increase in the frequency of intense tropical cyclones. Storm surges along the southern Chinese and northern and southern Vietnamese coastlines increase by up to 1 m, significantly larger than expected changes in mean sea-level rise over the same period. The length of coastline that is presently exposed to storm surge levels of 2.5 m or greater will more than double by 2050. Sections of Cambodian, Thai, and Malaysian coastlines are projected to experience storm surges (at higher return periods) in the future, not previously seen, due to a southward shift in tropical cyclone tracks. Given these findings, coastal flood management and adaptation in these areas should be reviewed for their resilience against future extreme sea levels

Simple design of broadband circularly polarized two-arm Archimedean spiral antenna

This article presents a broadband circularly polarized two-arm Archimedean spiral antenna with a novel simple feeding method. Contrary to the conventional spiral antenna excited by a vertically or horizontally balun, the presented design is directly fed by a coxial cable with a planar feeding section optimized to provide a broadband input impedance matching. An antenna prototype has been fabricated and measured to validate the concept. Measured results of reflection coefficient and axial ratio show a good performance over a frequency range from 1.0 to 3.5 GHz, equivalent to ~111.1%. Within this band, the antenna prototype exhibits bidirectional radiation with broadside gain values of better than 3.6 dBic