CCPValuesGenoAveragedByDog

Clinical Chemistry phenotype data averaged by dog for 335 dogs selected as clinically healthy with genotype data for ALT activity and Amylase activity

CCPSickDogs

Raw data from Clinical Chemistry Profile of dogs with diagnosed or presumed liver disease/injury

ccp_resid

Residual values for Clinical Chemistry Profile phenotypes from linear model after correcting for age, sex and spay/neuter status, and body weight covariates

cbc_dogs

Identification numbers of dogs for which Complete Blood Count data was included in the study

PhenotypeKeyCBCResid

Key for column names for CBC Residual text file

cbc_genos.bim

Bim file used in GWAS for CBC phenotypes

PlinkBreedFreqForALTAssocSNP

List of breeds shown in figure 3 along with number of chromosomes (column 2 ) and number of dogs (column 3) on which mean allele frequency for breed was based

PhenotypeKeyCCPResid

Key for column names for CCP Residual text file

cbc_genos.bed

Bed file used in GWAS for CBC phenotypes

Adhesion of tumor cells to protein-immobilized microfluidic channels under low shear (0.35dyn/cm²).

<p>Microfluidic channels were incubated with Protein G (100μg/ml), then anti-TF IgG (100μg/ml), or an anti-His antibody (100μg/ml) followed by TFPI (100μg/ml). Isotype IgG (100μg/ml) and anti-His IgG (100μg/ml) antibodies were used as negative control for anti-TF IgG and TFPI respectively. Tumor cells (1x10⁶cells/mL, pre-treated with 10nM FVIIa and 10nM FX for TFPI-coated channels) were introduced into the channels at 0.35dyn/cm² for 30 minutes, and non-specifically adhered cells were removed at 2.0dyn/cm². The entire channel was imaged to quantify the number of adherent cells. <b>A.</b> Representative bright field images of adherent tumor cells on channels immobilized with Protein G (negative control), anti-TF IgG and TFPI showing that more MDA-MB-231 than MCF-7 cells were bound to both anti-TF IgG- and TFPI-coated channels. <b>B.</b> The number of adherent cells was counted and normalized by the channel area. MDA-MB-231 showed significantly higher adhesion to TFPI- and anti-TF IgG-coated channels than MCF-7 (* p < 0.05, n = 4 for anti-TF IgG, n = 3 for TFPI). Significantly more MDA-MB-231 bound to TFPI- and anti-TF IgG-coated channels than negative controls (** p<0.05). <b>C.</b> MDA-MB-231 cells were pretreated with 50μg/ml anti-TF IgG (TF9-5B7 which blocks FVIIa binding to TF, or TF9-10H10 which does not block FVIIa binding to TF). The positive control had no antibody pretreatment, and isotype IgG pretreatment (50μg/ml) was used as a negative control. Blocking FVIIa binding to TF with TF9-5B7 antibody significantly decreased adhesion to TFPI-coated channels (* p < 0.05, n = 4). The observed decrease in MDA-MB-231 adhesion with the TF9-10H10 antibody, albeit not significant with this stringent statistical test, could be due to steric hindrance of TFPI binding to the TF/FVIIa/FXa complex on the tumor cells.</p