573 research outputs found
Role of erythroid Kruppel-like factor in human γ- to β-globin gene switching
Erythroid Kruppel-like factor (EKLF) is an erythroid-specific transcription factor that contains zinc finger domains similar to the Kruppel protein of Drosophila melanogaster. Previous studies demonstrated that EKLF binds to the CACCC box in the human β-globin gene promoter and activates transcription. CACCC box mutations that cause severe β-thalassemias in humans inhibit EKLF binding. Results described in this paper suggest that EKLF functions predominately in adult erythroid tissue. The EKLF gene is expressed at a 3-fold higher level in adult erythroid tissue than in fetal erythroid tissue, and the EKLF protein binds to the human β-globin promoter 8-fold more efficiently than to the human γ-globin promoter. Co-transfection experiments in the human fetal-like erythroleukemia cell line K562 demonstrate that over-expression of EKLF activates a β-globin reporter construct 1000-fold; a linked γ-globin reporter is activated only 3-fold. Mutation of the β-globin CACCC box severely inhibits activation. These results demonstrate that EKLF is a developmental stage-enriched protein that preferentially activates human β-globin gene expression. The data strongly suggest that EKLF is an important factor involved in human γ- to β-globin gene switching
Enhanced sensitivity to time-variation of m_p/m_e in the inversion spectrum of ammonia
We calculate the sensitivity of the inversion spectrum of ammonia to possible
time-variation of the ratio of the proton mass to the electron mass,
mu=m_p/m_e. For the inversion transition (lambda= 1.25 cm^{-1}) the relative
frequency shift is significantly enhanced: delta(omega)/omega=-4.46,
delta(mu)/mu. This enhancement allows one to increase sensitivity to the
time-variation of mu using NH_3 spectra for high redshift objects. We use
published data on microwave spectra of the object B0218+357 to place the limit
delta(mu)/mu =(0.6 +/- 1.9) 10^{-6} at redshift z=0.6847; this limit is several
times better than the limits obtained by different methods and may be
significantly improved. Assuming linear time dependence we obtain
dot{mu}/mu=(-1 +/- 3) 10^{-16} yr^{-1}
Constraining the variation of fundamental constants using 18cm OH lines
We describe a new technique to estimate variations in the fundamental
constants using 18cm OH absorption lines. This has the advantage that all lines
arise in the same species, allowing a clean comparison between the measured
redshifts. In conjunction with one additional transition (for example, an
HCO line), it is possible to simultaneously measure changes in ,
and . At present, only the 1665 MHz and 1667 MHz lines
have been detected at cosmological distances; we use these line redshifts in
conjunction with those of HI 21cm and mm-wave molecular absorption in a
gravitational lens at to constrain changes in the above three
parameters over the redshift range . While the constraints
are relatively weak ( 1 part in ), this is the first
simultaneous constraint on the variation of all three parameters. We also
demonstrate that either one (or more) of , and must vary with
cosmological time or there must be systematic velocity offsets between the OH,
HCO and HI absorbing clouds.Comment: 5 pages, no figures. Accepted for publication in Phys. Rev. Let
The Keck Aperture Masking Experiment: spectro-interferometry of 3 Mira Variables from 1.1 to 3.8 microns
We present results from a spectro-interferometric study of the Miras o Cet, R
Leo and W Hya obtained with the Keck Aperture Masking Experiment from 1998 Sep
to 2002 Jul. The spectrally dispersed visibility data permit fitting with
circularly symmetric brightness profiles such as a simple uniform disk. The
stellar angular diameter obtained over up to ~ 450 spectral channels spaning
the region 1.1-3.8 microns is presented. Use of a simple uniform disk
brightness model facilitates comparison between epochs and with existing data
and theoretical models. Strong size variations with wavelength were recorded
for all stars, probing zones of H2O, CO, OH, and dust formation. Comparison
with contemporaneous spectra extracted from our data show a strong
anti-correlation between the observed angular diameter and flux. These
variations consolidate the notion of a complex stellar atmosphere consisting of
molecular shells with time-dependent densities and temperatures. Our findings
are compared with existing data and pulsation models. The models were found to
reproduce the functional form of the wavelength vs. angular diameter curve
well, although some departures are noted in the 2.8-3.5 micron range.Comment: 10 pages, 10 figures Accepted to Ap
Conjugate 18cm OH Satellite Lines at a Cosmological Distance
We have detected the two 18cm OH satellite lines from the
source PKS1413+135, the 1720 MHz line in emission and the 1612 MHz line in
absorption. The 1720 MHz luminosity is , more than
an order of magnitude larger than that of any other known 1720 MHz maser. The
profiles of the two satellite lines are conjugate, implying that they arise in
the same gas. This allows us to test for any changes in the values of
fundamental constants, without being affected by systematic uncertainties
arising from relative motions between the gas clouds in which the different
lines arise. Our data constrain changes in ,
where ; we find ,
consistent with no changes in , and .Comment: 5 pages, 1 figure. Minor changes to match published versio
An upper limit on the evolution of carbon monoxide from Comet Kohoutek
The rate of evolution of CO from Comet Kohoutex is presented. The observations were made approximately two months after perihelion. The rate of evolution of CO at that time was apparently less than that of CH3CN observed before perihelion
Stern-Gerlach deflection of field-free aligned paramagnetic molecules
The effects of laser-induced prealignment on the deflection of paramagnetic
molecules by inhomogeneous static magnetic field are studied. Depending on the
relevant Hund's coupling case of the molecule, two different effects were
identified: either suppression of the deflection by laser pulses (Hund's
coupling case (a) molecules, such as ClO), or a dramatic reconstruction of the
broad distribution of the scattering angles into several narrow peaks (for
Hund's coupling case (b) molecules, such as O2 or NH). These findings are
important for various applications using molecular guiding, focusing and
trapping with the help of magnetic fields
Spin Relaxation Resonances Due to the Spin-Axis Interaction in Dense Rubidium and Cesium Vapor
Resonances in the magnetic decoupling curves for the spin relaxation of dense
alkali-metal vapors prove that much of the relaxation is due to the spin-axis
interaction in triplet dimers. Initial estimates of the spin-axis coupling
coefficients for the dimers are 290 MHz for Rb; 2500 MHz for Cs.Comment: submitted to Physical Review Letters, text + 3 figure
On spin-rotation contribution to nuclear spin conversion in C_{3v}-symmetry molecules. Application to CH_3F
The symmetrized contribution of E-type spin-rotation interaction to
conversion between spin modifications of E- and A_1-types in molecules with
C_{3v}-symmetry is considered. Using the high-J descending of collisional
broadening for accidental rotational resonances between these spin
modifications, it was possible to co-ordinate the theoretical description of
the conversion with (updated) experimental data for two carbon-substituted
isotopes of fluoromethane. As a result, both E-type spin-rotation constants are
obtained. They are roughly one and a half times more than the corresponding
constants for (deutero)methane.Comment: 13 pages with single-spacing, REVTeX, no figures, accepted for
publication in <J. Phys. B
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