Interaction of shallow cold surges with topography on scales of 100-1000 kilometers

December 1987.Includes bibliographical references.A shallow cold air mass is defined as one not extending to the top of the mountain ridge with which it interacts. The structure of such an airmass is examined using both observational data and a hydrostatic version of the Colorado State University Regional Atmospheric Modeling System. The prime constraint on a shallow cold surge is that the flow must ultimately be parallel to the mountain ridge. It is found that the effects of this constraint are altered significantly by surface sensible heat flux. Cold surges are slowed during the daylight hours, a result consistent with previous observational studies in Colorado east of the Continental Divide. Two case studies are described in detail, and several other events are cited. Since observations alone do not provide a complete description of diversion of the cold air by the mountain range, numerical model simulations provide additional insight into import ant mechanisms. A case study on 14 June 1985 is described using observational and model data. The model development of a deep boundary layer within the frontal baroclinic zone is consistent with the observations for this and other cases. This development is due to strong surface heating. Turning off the model shortwave radiation is seen to produce a rapid southward acceleration of the surface front, with very shallow cold air behind the front. Model simulations with specified surface temperature differences confirm the importance of upward heat flux from the surface in slowing the southward movement of the cold surge. It is concluded that the slowing is not due simply to the thermal wind developing in response to the heating of higher terrain to the west. Since surface heating is distributed over a deeper layer on the warm side of the temperature discontinuity, there is frontolysis at the surface. But this modification would develop even over flat terrain. Sloping terrain introduces additional effects. Heating at the western, upslope side of the cold surge inhibits the development of pressure gradients favorable to northerly flow. A second contribution comes from westerly winds at ridgetop level. These winds are heated over the higher terrain and flow downslope, further retarding the progression of the cold air at the surface.This research was supported by NOAA under Grant No. NE 85 RAH 05045 through CIRA

Climatology of the July 1981 surface flow over Northeast Colorado, A

September 1982.Includes bibliographical references.Sponsored by the Cooperative Institute for Research in the Atmosphere at Colorado State University.Sponsored by the Environmental Research Laboratories of the National Oceanic and Atmospheric Administration NA81RA-H-00001, Amend. 2, item 4.Appendix A: Surface Mesonet Stations

The Emperor\u27s New Clothes: A Survey of Significant Court Decisions Interpreting Pennsylvania\u27s Sovereign Immunity Act and Its Waivers

This article surveys and analyzes the history of sovereign immunity in the Commonwealth of Pennsylvania. The article traces the adoption of sovereign immunity, its abrogation by the Pennsylvania Supreme Court, and the General Assembly\u27s reaffirmation of the doctrine in the Sovereign Immunity Act. The article provides a review of the important sections of the Sovereign Immunity Act and examines many of the appellate decisions which have addressed the Act and the waivers of immunity contained therein

Molecular Line Observations of the Small Protostellar Group L1251B

We present molecular line observations of L1251B, a small group of pre- and protostellar objects, and its immediate environment in the dense C18O core L1251E. These data are complementary to near-infrared, submillimeter and millimeter continuum observations reported by Lee et al. (2006, ApJ, 648, 491; Paper I). The single-dish data of L1251B described here show very complex kinematics including infall, rotation and outflow motions, and the interferometer data reveal these in greater detail. Interferometer data of N2H+ 1-0 suggest a very rapidly rotating flattened envelope between two young stellar objects, IRS1 and IRS2. Also, interferometer data of CO 2-1 resolve the outflow associated with L1251B seen in single-dish maps into a few narrow and compact components. Furthermore, the high resolution data support recent theoretical studies of molecular depletions and enhancements that accompany the formation of protostars within dense cores. Beyond L1251B, single-dish data are also presented of a dense core located ~150" to the east that, in Paper I, was detected at 850 micron but has no associated point sources at near- and mid-infrared wavelengths. The relative brightness between molecules, which have different chemical timescales, suggests it is less chemically evolved than L1251B. This core may be a site for future star formation, however, since line profiles of HCO+, CS, and HCN show asymmetry with a stronger blue peak, which is interpreted as an infall signature.Comment: 46 pages, 18 figures. Accepted for publication in Ap

Sulphur hexaflouride: low energy (e,2e) experiments and molecular three-body distorted wave theory

Experimental and theoretical triple differential ionisation cross-sections (TDCS’s) are presented for the highest occupied molecular orbital of sulphur hexafluoride. These measurements were performed in the low energy regime, with outgoing electron energies ranging from 5 to 40 eV in a coplanar geometry, and with energies of 10 and 20 eV in a perpendicular geometry. Complementary theoretical predictions of the TDCS were calculated using the molecular three-body distorted wave formalism. Calculations were performed using a proper average over molecular orientations as well as the orientation-averaged molecular orbital approximation. This more sophisticated model was found to be in closer agreement with the experimental data, however neither model accurately predicts the TDCS over all geometries and energies

Pim kinases phosphorylate multiple sites on Bad and promote 14-3-3 binding and dissociation from Bcl-X(L)

BACKGROUND: Pim-1, 2 and 3 are a group of enzymes related to the calcium calmodulin family of protein kinases. Over-expression of Pim-1 and Pim-2 in mice promotes the development of lymphomas, and up-regulation of Pim expression has been observed in several human cancers. RESULTS: Here we show that the pim kinases are constitutively active when expressed in HEK-293 cells and are able to phosphorylate the Bcl-2 family member Bad on three residues, Ser112, Ser136 and Ser155 in vitro and in cells. In vitro mapping showed that Pim-2 predominantly phosphorylated Ser112, while Pim-1 phosphorylated Ser112, but also Ser136 and Ser155 at a reduced rate compared to Ser112. Pim-3 was found to be the least specific for Ser112, and the most effective at phosphorylating Ser136 and Ser155. Pim-3 was also able to phosphorylate other sites in Bad in vitro, including Ser170, another potential in vivo site. Mutation of Ser136 to alanine prevented the phosphorylation of Ser112 and Ser155 by Pim kinases in HEK-293 cells, suggesting that this site must be phosphorylated first in order to make the other sites accessible. Pim phosphorylation of Bad was also found to promote the 14-3-3 binding of Bad and block its association with Bcl-X(L). CONCLUSION: All three Pim kinase family members predominantly phosphorylate Bad on Ser112 and in addition are capable of phosphorylating Bad on multiple sites associated with the inhibition of the pro-apoptotic function of Bad in HEK-293 cells. This would be consistent with the proposed function of Pim kinases in promoting cell proliferation and preventing cell death

Induction of the cell survival kinase Sgk1: A possible novel mechanism for α-phenyl-N-tert-butyl nitrone in experimental stroke.

Nitrones (e.g. α-phenyl-N-tert-butyl nitrone; PBN) are cerebroprotective in experimental stroke. Free radical trapping is their proposed mechanism. As PBN has low radical trapping potency, we tested Sgk1 induction as another possible mechanism. PBN was injected (100 mg/kg, i.p.) into adult male rats and mice. Sgk1 was quantified in cerebral tissue by microarray, quantitative RT-PCR and western analyses. Sgk1+/+ and Sgk1-/- mice were randomized to receive PBN or saline immediately following transient (60 min) occlusion of the middle cerebral artery. Neurological deficit was measured at 24 h and 48 h and infarct volume at 48 h post-occlusion. Following systemic PBN administration, rapid induction of Sgk1 was detected by microarray (at 4 h) and confirmed by RT-PCR and phosphorylation of the Sgk1-specific substrate NDRG1 (at 6 h). PBN-treated Sgk1+/+ mice had lower neurological deficit ( p < 0.01) and infarct volume ( p < 0.01) than saline-treated Sgk1+/+ mice. PBN-treated Sgk1-/- mice did not differ from saline-treated Sgk1-/- mice. Saline-treated Sgk1-/- and Sgk1+/+ mice did not differ. Brain Sgk3:Sgk1 mRNA ratio was 1.0:10.6 in Sgk1+/+ mice. Sgk3 was not augmented in Sgk1-/- mice. We conclude that acute systemic treatment with PBN induces Sgk1 in brain tissue. Sgk1 may play a part in PBN-dependent actions in acute brain ischemia

A Stochastic Analysis of Pumping Tests in Laterally Nonuniform Media

This is the published version. Copyright American Geophysical UnionConventional pumping test analysis methodology assumes that aquifer transmissivity is invariant in space. The ramifications of this assumption are examined for hypothetical units whose variations in transmissivity are considered to be reasonable representations of variations possible in natural systems. The dependence of pumping test transmissivity on spatial (angular and radial) and temporal location of observations and the method of drawdown analysis is assessed. The dependence on the angular position of an observation well appears of little significance. Dependence on radial position, however, can be strong. The dependence on the interval of time used in the pumping test analysis is only important in highly variable systems. Methods of drawdown analysis that yield identical estimates in uniform units yield differing estimates in nonuniform ones as a result of a difference in data-fitting procedures. A reasonable estimate of transmissivity at a regional scale can be obtained from a Cooper-Jacob analysis applied at a large duration of pumpage. In general, conventional approaches for pumping test analysis should be viable in the nonuniform aquifers of the type considered here

Sleep loss drives acetylcholine- and somatostatin interneuron-mediated gating of hippocampal activity to inhibit memory consolidation

Sleep loss disrupts consolidation of hippocampus-dependent memory. To characterize effects of learning and sleep loss, we quantified activity-dependent phosphorylation of ribosomal protein S6 (pS6) across the dorsal hippocampus of mice. We find that pS6 is enhanced in dentate gyrus (DG) following single-trial contextual fear conditioning (CFC) but is reduced throughout the hippocampus after brief sleep deprivation (SD; which disrupts contextual fear memory [CFM] consolidation). To characterize neuronal populations affected by SD, we used translating ribosome affinity purification sequencing to identify cell type-specific transcripts on pS6 ribosomes (pS6-TRAP). Cell type-specific enrichment analysis revealed that SD selectively activated hippocampal somatostatin-expressing (Sst+) interneurons and cholinergic and orexinergic hippocampal inputs. To understand the functional consequences of SD-elevated Sst+ interneuron activity, we used pharmacogenetics to activate or inhibit hippocampal Sst+ interneurons or cholinergic input from the medial septum. The activation of either cell population was sufficient to disrupt sleep-dependent CFM consolidation by gating activity in granule cells. The inhibition of either cell population during sleep promoted CFM consolidation and increased S6 phosphorylation among DG granule cells, suggesting their disinhibition by these manipulations. The inhibition of either population across post-CFC SD was insufficient to fully rescue CFM deficits, suggesting that additional features of sleeping brain activity are required for consolidation. Together, our data suggest that state-dependent gating of DG activity may be mediated by cholinergic input and local Sst+ interneurons. This mechanism could act as a sleep loss-driven inhibitory gate on hippocampal information processing.</p