Rapid Detection of Intracellular p47phox and p67phox by Flow Cytometry; Useful Screening Tests for Chronic Granulomatous Disease

Chronic granulomatous disease (CGD) is caused by defects of NADPH oxidase. The diagnosis of CGD can be made by analysis of NADPH oxidase activity, however, identification of the CGD subgroups is required before performing mutation analysis. The membrane-bound subunits, gp91phox and p22phox, can be quickly analyzed by flow cytometry, unlike the cytosolic components, p47phox and p67phox. We evaluated the feasibility of flow cytometric detection of p47phox and p67phox with specific monoclonal antibodies in two patients with p47phox deficiency and 7 patients with p67phox deficiency. Consistent with previous observations, p47phox and p67phox were expressed in phagocytes and B cells, but not in T or natural killer cells, from normal controls. In contrast, patients with p47phox and p67phox deficiency showed markedly reduced levels of p47phox and p67phox, respectively. These techniques will be useful to rapidly assess the expression of the cytosolic components, p47phox and p67phox, and represents important secondary screening tests for CGD. © 2013 Springer Science+Business Media New York

Sea surface temperature intercomparison in the framework of the Copernicus Climate Change Service (C3S)

A joint effort between the Copernicus Climate Change Service (C3S) and the Group for High Resolution Sea Surface Temperature (GHRSST) has been dedicated to an intercomparison study of eight global gap-free Sea Surface Temperature (SST) products to assess their accurate representation of the SST relevant to climate analysis. In general, all SST products show consistent spatial patterns and temporal variability during the overlapping time period (2003-2018). The main differences between each product are located in western boundary current and Antarctic Circumpolar Current regions. Linear trends display consistent SST spatial patterns among all products and exhibit a strong warming trend from 2012 to 2018 with the Pacific Ocean basin as the main contributor. SST discrepancy between all SST products is very small compared to the significant warming trend. Spatial power spectral density shows that the interpolation into 1o spatial resolution has negligible impacts on our results. The global mean SST time series reveals larger differences among all SST products during the early period of the satellite era (1982-2002) when there were fewer observations, indicating that the observation frequency is the main constraint of the SST climatology. The maturity matrix scores, which present the maturity of each product in terms of documentation, storage, and dissemination but not the scientific quality, demonstrate that ESA-CCI and OSTIA SST are well documented for users' convenience. Improvements could be made for MGDSST and BoM SST. Finally, we have recommended that these SST products can be used for fundamental climate applications and climate studies (e.g. El Nino)

Structural Diversity of Ubiquitin E3 Ligase

The post-translational modification of proteins regulates many biological processes. Their dysfunction relates to diseases. Ubiquitination is one of the post-translational modifications that target lysine residue and regulate many cellular processes. Three enzymes are required for achieving the ubiquitination reaction: ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3). E3s play a pivotal role in selecting substrates. Many structural studies have been conducted to reveal the molecular mechanism of the ubiquitination reaction. Recently, the structure of PCAF_N, a newly categorized E3 ligase, was reported. We present a review of the recent progress toward the structural understanding of E3 ligases

A substantial incidence of silent short segment endoscopically suspected esophageal metaplasia in an adult Japanese primary care practice

AIM: To determine the incidence and characteristics of endoscopically suspected esophageal metaplasia (ESEM) in a primary adult care institution

Correction to Synthetic Studies on (+)-Manzamine A: Stereoselective Synthesis of the Tetracyclic Core Framework

Correction to Synthetic Studies on (+)-Manzamine A: Stereoselective Synthesis of the Tetracyclic Core Framewor

Coronary flow velocity immediately after primary coronary stenting as a predictor of ventricular wall motion recovery in acute myocardial infarction

AbstractOBJECTIVESThe purpose of this study was to examine the relationship between the pattern of coronary blood flow velocity immediately after successful primary stenting and the recovery of left ventricular (LV) wall motion in patients with acute myocardial infarction (AMI).BACKGROUNDIt is difficult to predict the recovery of LV wall motion immediately after direct angioplasty in AMI. Recent reports indicate that dysfunctional coronary microcirculation is an important determinant of prognosis for AMI patients after successful reperfusion.METHODSWe measured left anterior descending coronary flow velocity variables using a Doppler guide wire immediately after successful primary stenting in 31 patients with their first anterior AMI. The patients were divided into two groups: those with and those without early systolic reverse flow (ESRF). Changes in LV regional wall motion (RWM) and ejection fraction (EF) at admission and at discharge were compared between the two groups. Coronary flow velocity variables immediately after primary stenting were compared with changes in left ventriculographic indexes.RESULTSThe change in RWM was significantly greater in the non-ESRF group than it was in the ESRF group (0.9 ± 0.7 vs. −0.1 ± 0.3 standard deviation/chord, respectively, p < 0.001). The change in EF was also significantly greater in the non-ESRF group than it was in the ESRF group (10 ± 10 vs. 1 ± 6%, respectively, p < 0.05). In the non-ESRF group (diastolic to systolic velocity ratio [DSVR] <3.0), the DSVR correlated positively with the change in RWM (r = 0.60, p < 0.005, n = 24) and the change in EF (r = 0.52, p < 0.01).CONCLUSIONSThe coronary flow velocity pattern measured immediately after successful primary stenting is predictive of the recovery of regional and global LV function in patients with AMI