125 research outputs found
MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts
AbstractMesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and osteoblast differentiation, we examined expression of MFH-1 induced by bone morphogenetic protein-2 (BMP-2) in C2C12 myoblasts, and found that MFH-1 protein and also MFH-1 mRNA increased markedly in C2C12 cells after treatment with BMP-2. To confirm the hypothesis that BMP-2 induced osteoblastic differentiation of C2C12 cells by increasing MFH-1 expression, we lowered the endogenous MFH-1 level by stably transfecting C2C12 cells with antisense MFH-1 sequence, and found that in antisense MFH-1 cell lines, both alkaline phosphatase (ALP) activity and production of osteocalcin induced by BMP-2 decreased markedly in comparison with control cell lines. Our results suggest that the BMP-2-induced MFH-1 protein may play a key role in regulating the commitment to osteoblastic differentiation of C2C12 myoblasts and production of osteoblast markers including ALP and osteocalcin
Development of the analog ASIC for multi-channel readout X-ray CCD camera
We report on the performance of an analog application-specific integrated
circuit (ASIC) developed aiming for the front-end electronics of the X-ray
CCDcamera system onboard the next X-ray astronomical satellite, ASTRO-H. It has
four identical channels that simultaneously process the CCD signals.
Distinctive capability of analog-to-digital conversion enables us to construct
a CCD camera body that outputs only digital signals. As the result of the
front-end electronics test, it works properly with low input noise of =<30 uV
at the pixel rate below 100 kHz. The power consumption is sufficiently low of
about 150 mW/chip. The input signal range of 720 mV covers the effective energy
range of the typical X-ray photon counting CCD (up to 20 keV). The integrated
non-linearity is 0.2% that is similar as those of the conventional CCDs in
orbit. We also performed a radiation tolerance test against the total ionizing
dose (TID) effect and the single event effect. The irradiation test using 60Co
and proton beam showed that the ASIC has the sufficient tolerance against TID
up to 200 krad, which absolutely exceeds the expected amount of dose during the
period of operating in a low-inclination low-earth orbit. The irradiation of Fe
ions with the fluence of 5.2x10^8 Ion/cm2 resulted in no single event latchup
(SEL), although there were some possible single event upsets. The threshold
against SEL is higher than 1.68 MeV cm^2/mg, which is sufficiently high enough
that the SEL event should not be one of major causes of instrument downtime in
orbit.Comment: 16 pages, 6 figure
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