PERKEMBANGAN KEMAMPUAN MEMBACA PADA ANAK USIA PRASEKOLAH : Studi Observasi Terhadap Seorang Anak Usia Prasekolah yang Belajar Membaca Berdasarkan Pendekatan Pengalaman Berbahasa

The increasing popularity of essential oils for skincare has led to investigation of their biological effects in human skin cells. In this study, we investigated the biological activities of three commercially available essential oils, i.e. rosemary oil, wild orange oil, and a blend (commercial name: Deep Blue) composed of oils from wintergreen, camphor, peppermint, blue tansy, German chamomile, Helichrysum, and Osmanthus, in a pre-inflamed human dermal fibroblast culture model, simulating chronic inflammation. The impact of essential oils on proteins associated with inflammation and tissue remodeling and on the genome-wide expression of 21,224 genes was investigated. The three essential oils diversely modulated global gene expression. Ingenuity Pathway Analysis showed that the oils affected numerous critical genes and signaling pathways. Specifically, rosemary oil influenced processes involved in cancer signaling and metabolism; orange oil affected processes related to cancer signaling, immunomodulation, and metabolism; the blend influenced inflammation, immunomodulation, and wound healing. These findings are largely consistent with the existing literature, supporting the beneficial biological activities of these essential oils. Our study provides the first evidence indicating how these essential oils affect genome-wide gene expression in human skin cells and establishes a basis for further research into their biological mechanisms of action

The Effects of Processing on the Nutritional Benefits of Fruit: Grapes, Raisins, and Papaya

173 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2008.Each component of a food can serve a beneficial, neutral or harmful function within the food and after consumption. Changes that occur during food, digestive, or absorptive processing modify the human nutritional potential of a food by changing food components or interactions between food components. This hypothesis was tested with grapes and raisins to explore changes due to drying on serum antioxidant capacity, with papaya pulp to improve availability and human nutrition potential using multiple processing methods, and with individual components found in food and serum to evaluate interactions and synergistic potential. There is a long-term antioxidant benefit to consuming a serving of grapes or raisins in addition to a free-living, varied diet. However, within two hours after consuming bagels and grapes or raisins, there was an environment of increased oxidation in serum. Repeated exposure to postprandial oxidation may be a long-term contributor to cardiovascular disease. Improving availability of nutrient dense foods could help combat postprandial oxidation. Papaya is nutrient dense, but not readily available in a processed form. Producing a papaya pulp beverage that retains its fresh flavor and nutritional value is not possible with traditional pasteurization. Combining moderate irradiation and mild heat resulted in a better tasting, nutritionally optimized papaya pulp beverage meeting microbiological and enzyme standards for a refrigerated product. Chemical changes that occur as papaya undergoes processing led to the hypothesis that there may be certain combinations of chemicals that would provide more effective antioxidative benefits than others. Combining individual phenols, sugars and ascorbic acid at concentrations found in honey and papaya in increasingly complex mixtures suggested some combinations had synergistic potential. Similar results were found after simulated digestion. Serum-achievable concentrations of a separate set of compounds also suggested synergistic potential. These results support our hypothesis. The changes that occur during processing can affect the human nutritional potential of food and interactions are important. Further study is needed to understand these complex changes and interactions.U of I OnlyRestricted to the U of I community idenfinitely during batch ingest of legacy ETD

Biological activity of vetiver (Vetiveria zizanioides) essential oil in human dermal fibroblasts

Vetiver (Vetiveria zizanioides) essential oil (VEO) has a long history of use. However, research on its biological activity in human skin cells is scarce. In this study, we investigated the biological activity of VEO in a pre-inflamed human dermal fibroblast model, which was designed to mimic the disease biology of chronic inflammation and fibrosis. We analyzed the impact of VEO on the levels of 17 important protein biomarkers pertinent to immune response and tissue remodeling. VEO exhibited strong antiproliferative activity in these cells and significantly inhibited the production of collagen III, an important molecule for skin and tissue remodeling processes. We also studied the effect of VEO on regulating genome-wide gene expression. VEO robustly impacted many genes and signaling pathways that are closely related to tissue remodeling and metabolism, among others. Specifically, VEO significantly impacted pathways for cholesterol synthesis and metabolism. This study provides the first evidence of the biological activity of VEO in human dermal fibroblasts. Though a definite conclusion remains elusive, the data suggest that VEO has therapeutic potential for both cosmetic and metabolic health care products. Further research into VEO’s biological and pharmacological mechanisms of action is recommended

Anti-inflammatory activity of Juniper (Juniperus communis) berry essential oil in human dermal fibroblasts

Although juniper (Juniperus communis) berry essential oil (JEO) has been used in skin care products, research on its biological activity in human skin cells is scarce. In the current study, we explored the biological activity of JEO (with alpha-pinene as the major active component) in pre-inflamed human dermal fibroblasts, which were designed to mimic the disease biology of chronic inflammation and fibrosis. We analyzed the levels of 17 important protein biomarkers pertinent to inflammation and tissue remodeling. JEO exhibited robust antiproliferative activity and significantly inhibited the increased production of the proinflammatory chemokines interferon gamma-induced protein 10 (IP-10) and interferon-inducible T-cell alpha chemoattractant (I-TAC). Additionally, JEO significantly inhibited tissue remodeling biomarkers, namely collagen I, collagen III, and plasminogen activator inhibitor 1 (PAI-I). Macrophage colony-stimulating factor (M-CSF), an immunomodulatory protein molecule, was also significantly downregulated by JEO. Moreover, we found that JEO robustly modulated global gene expression. Ingenuity Pathway Analysis also showed that JEO affected many important signaling pathways that are closely related to metabolism, inflammation, immune response, wound healing, and cancer biology. This study provides the first evidence of the biological activity of JEO in human dermal fibroblasts. Thus, JEO is a promising therapeutic candidate for inflammatory conditions in the skin

Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts

Context: Clove (Eugenia caryophyllata Thunb. [Myrtaceae]) essential oil (CEO) has been shown to possess antimicrobial, antifungal, antiviral, antioxidant, anti-inflammatory and anticancer properties. However, few studies have focused on its topical use. Objective: We investigated the biological activity of a commercially available CEO in a human skin disease model. Materials and methods: We evaluated the effect of CEO on 17 protein biomarkers that play critical roles in inflammation and tissue remodelling in a validated human dermal fibroblast system, which was designed to model chronic inflammation and fibrosis. Four concentrations of CEO (0.011, 0.0037, 0.0012, and 0.00041%, v/v) were studied. The effect of 0.011% CEO on genome-wide gene expression was also evaluated. Results and discussion: CEO at a concentration of 0.011% showed robust antiproliferative effects on human dermal fibroblasts. It significantly inhibited the increased production of several proinflammatory biomarkers such as vascular cell adhesion molecule-1 (VCAM-1), interferon γ-induced protein 10 (IP-10), interferon-inducible T-cell α chemoattractant (I-TAC), and monokine induced by γ interferon (MIG). CEO also significantly inhibited tissue remodelling protein molecules, namely, collagen-I, collagen-III, macrophage colony-stimulating factor (M-CSF), and tissue inhibitor of metalloproteinase 2 (TIMP-2). Furthermore, it significantly modulated global gene expression and altered signalling pathways critical for inflammation, tissue remodelling, and cancer signalling processes. CEO significantly inhibited VCAM-1 and collagen III at both protein and gene expression levels. Conclusions: This study provides important evidence of CEO-induced anti-inflammatory and tissue remodelling activity in human dermal fibroblasts. This study also supports the anticancer properties of CEO and its major active component eugenol

Cardamom (Elettaria cardamomum) essential oil significantly inhibits vascular cell adhesion molecule 1 and impacts genome-wide gene expression in human dermal fibroblasts

Cardamom (Elettaria cardamomum) essential oil (CEO) is popular in skin care, although no studies have reported its biological activity in human skin cells. We studied its effect on 17 protein biomarkers closely related to inflammation, immune responses, and tissue remodeling using a dermal fibroblast cell culture system designed to model chronic inflammation. CEO significantly inhibited the proliferation of skin cells and the expression of vascular cell adhesion molecule 1 (VCAM-1) and macrophage colonystimulating factor (M-CSF). The CEO-induced inhibition of the production of these protein biomarkers suggests its anti-inflammatory and immunomodulatory potential, which has been largely attributed to its major active component, eucalyptol. We further studied the effect of CEO on the expression of 21,224 genes in the same cell culture system. Ingenuity pathway analysis showed that CEO affected critical genes and signaling pathways closely involved in inflammation, immune responses, and tissue remodeling. The observed overall CEO-induced inhibition of these genes and pathways supports its anti-inflammatory and immunomodulatory properties. This study provides important evidence of the biological activity of CEO in human skin cells. Further research into the mechanism of action of CEO in human skin and other systems is recommended

An essential oil blend modulates important inflammation- and immune response-related biomarkers in human cell cocultures

Despite growing scientific evidence that essential oils possess important therapeutic benefits, research on their biological activities in complex human disease models is scarce. To enhance understanding in this regard, we analyzed the biological activities of an essential oil blend (EOB) in validated human cocultures with or without tumor cells. These disease models allow for measurement of changes in protein biomarkers induced by EOB treatment. This EOB is primarily composed of essential oils from frankincense resin, sweet orange peel, litsea fruit, thyme plant oil, clove bud, summer savory plant, and niaouli leaf. EOB showed significant effects on levels of important biomarkers related to inflammation, immune response, tissue remodeling, and tumor biology. In tumor cocultures, EOB treatment resulted in elevated inflammation- and immune-related biomarkers, including soluble interleukin (sIL)-17A, sIL-2, sIL-6, vascular cell adhesion molecule-1 (VCAM-1), cluster of differentiation (CD)40, CD69, soluble granzyme B (sGranB), and soluble interferon-gamma (sIFN-γ). However, several of these same biomarkers were decreased in EOB-treated nontumor cell cocultures, suggesting that EOB exhibits tumor-specific immune enhancement. In conclusion, EOB may potentially impact human cells through anti-inflammatory activities, immune enhancing functions, and modulation of wound healing

Anti-inflammatory activity of Juniper (<i>Juniperus communis</i>) berry essential oil in human dermal fibroblasts

<p>Although juniper (<i>Juniperus communis</i>) berry essential oil (JEO) has been used in skin care products, research on its biological activity in human skin cells is scarce. In the current study, we explored the biological activity of JEO (with alpha-pinene as the major active component) in pre-inflamed human dermal fibroblasts, which were designed to mimic the disease biology of chronic inflammation and fibrosis. We analyzed the levels of 17 important protein biomarkers pertinent to inflammation and tissue remodeling. JEO exhibited robust antiproliferative activity and significantly inhibited the increased production of the proinflammatory chemokines interferon gamma-induced protein 10 (IP-10) and interferon-inducible T-cell alpha chemoattractant (I-TAC). Additionally, JEO significantly inhibited tissue remodeling biomarkers, namely collagen I, collagen III, and plasminogen activator inhibitor 1 (PAI-I). Macrophage colony-stimulating factor (M-CSF), an immunomodulatory protein molecule, was also significantly downregulated by JEO. Moreover, we found that JEO robustly modulated global gene expression. Ingenuity Pathway Analysis also showed that JEO affected many important signaling pathways that are closely related to metabolism, inflammation, immune response, wound healing, and cancer biology. This study provides the first evidence of the biological activity of JEO in human dermal fibroblasts. Thus, JEO is a promising therapeutic candidate for inflammatory conditions in the skin.</p