Effects of long term application of compost and poultry manure on soil quality of citrus orchards in Southern Italy.

A six-year study was carried out in an organically managed orange orchard located in Sicily (Southern Italy) to assess the effect of compost and organic fertilizers utilisation on soil quality. Adopting a randomized-block experimental design with three replicates, four treatments were carried out. In treatments 1 and 2, two different composts (C1 from distillery by products and C2 from livestock wastes) were applied. The plots of treatment 3 were fertilized using dried poultry manure. The control treatment was fertilized with mineral/synthetic fertilizers. In order to verify the hypothesis that composts and organic fertilizers improve soil fertility, soil quality was evaluated by selecting dynamic soil parameters, as indicators linked to C and N cycles. Total organic C, total N, C/N ratio, humified fraction, isoelectric focusing (IEF) of extracted organic matter, microbial biomass C, potentially mineralisable N under anaerobic conditions, potenzially mineralizable C, C mineralization quotient and metabolic quotient were determined for each sample. Furthermore, the Comunity level Physiological Profile (by Biolog tecnique) was defined, calculating derived functional biodiversity and versatility indexes. Parameters related to IEF and potentially mineralizable C showed significant differences among the treatments. Moreover, total C, total N and humification parameters tended to increase, while no differences were observed in biodiversity indexes. On these findings, it was concluded that composts and poultry manure only weakly affected soil properties, though they increased soil nutritive elements potentially available to crops

Dbl oncogene expression in MCF-10 A epithelial cells disrupts mammary acinar architecture, induces EMT and angiogenic factor secretion.

The proteins of the Dbl family are guanine nucleotide exchange factors (GEFs) of Rho GTPases and are known to be involved in cell growth regulation. Alterations of the normal function of these proteins lead to pathological processes such as developmental disorders, neoplastic transformation, and tumor metastasis. We have previously demonstrated that expression of Dbl oncogene in lens epithelial cells modulates genes encoding proteins involved in epithelial-mesenchymal-transition (EMT) and induces angiogenesis in the lens. Our present study was undertaken to investigate the role of Dbl oncogene in epithelial cells transformation, providing new insights into carcinoma progression. To assess how Dbl oncogene can modulate EMT, cell migration, morphogenesis, and expression of pro-apoptotic and angiogenic factors we utilized bi- and three-dimensional cultures of MCF-10░A cells. We show that upon Dbl expression MCF-10░A cells undergo EMT. In addition, we found that Dbl overexpression sustain

Rapid detection of copy number variations and point mutations in BRCA1/2 genes using a single workflow by ion semiconductor sequencing pipeline

Molecular analysis of BRCA1 (MIM# 604370) and BRCA2 (MIM #600185) genes is essential for familial breast and ovarian cancer prevention and treatment. An efficient, rapid, cost-effective accurate strategy for the detection of pathogenic variants is crucial. Mutations detection of BRCA1/2 genes includes screening for single nucleotide variants (SNVs), small insertions or deletions (indels), and Copy Number Variations (CNVs). Sanger sequencing is unable to identify CNVs and therefore Multiplex Ligation Probe amplification (MLPA) or Multiplex Amplicon Quantification (MAQ) is used to complete the BRCA1/2 genes analysis. The rapid evolution of Next Generation Sequencing (NGS) technologies allows the search for point mutations and CNVs with a single platform and workflow. In this study we test the possibilities of NGS technology to simultaneously detect point mutations and CNVs in BRCA1/2 genes, using the OncomineTM BRCA Research Assay on Personal Genome Machine (PGM) Platform with Ion Reporter Software for sequencing data analysis (Thermo Fisher Scientific). Comparison between the NGS-CNVs, MLPA and MAQ results shows how the NGS approach is the most complete and fast method for the simultaneous detection of all BRCA mutations, avoiding the usual time consuming multistep approach in the routine diagnostic testing of hereditary breast and ovarian cancers

M13 phages uptake of gold nanoparticles for radio-and thermal-therapy and contrast imaging improvement

The presented work deals with the uptake of gold nanoparticles (Au NPs) by M13 phages in solutions. In particular, the Au NPs uptake modalities and their localization in the filamentous phages are evaluated and measured. Gold spherical nanoparticles (with an average diameter of the order of 10 nm) are obtained by laser ablation in water with a sodium citrated surfactant. The interest of such application comes from the possibility to employ living biological structures to transport heavy metallic nanoparticles inside cells of tumoral tissues. Indeed, phages have the capability to introduce Au NPs in the proximity to the cell nucleus, increasing the efficiency of DNA destruction in the tumoral cells by employing low doses of ionizing radiation during radiotherapy and hyperthermia treatments. Several analyses and microscopy characterizations of the prepared phages samples embedding gold nanoparticles are presented, demonstrating that the presence of Au NPs increases the phages imaging contrast

When do spinouts benefit from market overlap with parent firms?

We examine how market overlap with parent organizations impacts the performance of startups founded by the former employees of these incumbent firms. Building on knowledge inheritance and competitive dynamics theories, we propose that the degree to which the operating markets of spinouts overlap with their parent organizations has a curvilinear relationship with their likelihood of survival. Market overlap is beneficial to spinouts because it reduces uncertainty during the early stages of new venture development. However, substantial market overlap may spark hostile actions by the parent organizations, thereby creating disruptive competition that may lower the likelihood of spinouts' survival. Furthermore, we hypothesize that the previous hierarchical position of founders in parent organizations moderates the overlap–performance relationship. Using a sample of European biotech spinouts and their parent firms, we find support for our hypotheses

Seed morphobiometry of wild and cultivated taxa of Phaseolus L. (Fabaceae)

A morphobiometric analysis of seeds of Phaseolus L. from 50 populations belonging to 31 wild and cultivated taxa was carried out. Based on the outcome of the study an identifi cation key was developed comprising 25 morphotypes of which 23 related to individual taxa The different patterns of seminal tegument allowed 31 taxa to cluster into three groups: (1) Phaseolus angustissimus Gray group (wrinkled seed coat) with two morphotypes, (2) Phaseolus lunatus L. group (smooth tegument with striae) with ten morphotypes and (3) Phaseolus vulgaris L. group (smooth tegument without striae) with 13 morphotypes. All the taxa exhibited uniformity in size and variability in tegument colour of seeds irrespective of the source of population and the type of habitat. Characterization of taxa into defi nite morphotypes and the groups could be useful for biosystematic investigations and the markerbased genetic selection approaches in this important leguminous crop

Fibre electronics: towards scaled-up manufacturing of integrated e-textile systems

The quest for a close human interaction with electronic devices for healthcare, safety, energy and security has driven giant leaps in portable and wearable technologies in recent years. Electronic textiles (e-textiles) are emerging as key enablers of wearable devices. Unlike conventional heavy, rigid, and hard-to-wear gadgets, e-textiles can lead to lightweight, flexible, soft, and breathable devices, which can be worn like everyday clothes. A new generation of fibre-based electronics is emerging which can be made into wearable e-textiles. A suite of start-of-the-art functional materials have been used to develop novel fibre-based devices (FBDs), which have shown excellent potential in creating wearable e-textiles. Recent research in this area has led to the development of fibre-based electronic, optoelectronic, energy harvesting, energy storage, and sensing devices, which have also been integrated into multifunctional e-textile systems. Here we review the key technological advancements in FBDs and provide an updated critical evaluation of the status of the research in this field. Focusing on various aspects of materials development, device fabrication, fibre processing, textile integration, and scaled-up manufacturing we discuss current limitations and present an outlook on how to address the future development of this field. The critical analysis of key challenges and existing opportunities in fibre electronics aims to define a roadmap for future applications in this area