Supplemental_File4_Protein_UCP1_Query_for_TBLASTN

The UCP1 protein sequences used for tBLASTn against the genomes

Supplemental_File3_BlastQUERY_Synteny

The exon sequences for ucp1 and six flanking genes used in the BLASTn for synteny analysis

Supplemental_File6_BlastQUERY_for_raw_reads_reptiles_birds

The ucp1 gene sequences used for BLASTn against the raw sequence data for reptiles

Supplemental_File5_BlastQUERY_for_raw_reads_mammals

The ucp1 gene sequences used for BLASTn against the raw sequence data for reptiles

Supplemental_File1_Methods_Results_Figures

Detailed description of methods and results including figures S1 and S2

Relationship showing a negative relationship between TL and age in sire males (r_s = −0.34, p = 0.03, n = 40).

<p>Relationship showing a negative relationship between TL and age in sire males (r_s = −0.34, p = 0.03, n = 40).</p

Telomeric profiles of sand lizards.

<p>Telomere fragments (lanes 1–14) were size-separated on a non-denaturing agarose gel together with two size standards (lane M, second standard not shown) and detected using chemiluminescence. The mean length of TRFs was estimated within the outlined window using the size standards (fragment sizes shown in kilobases, see Methods for details). The rectangle specifies the area used for background subtraction.</p

Data output corresponding to samples (lane 1–14) shown in Supplement 1.

<p>Statistical measurements produced by TELOMETRIC include the mean, median, mode, variance, and semi-interquartile range (SIR). The latter gives the spread in kilobases (kb) spanning the 25th to 75th percentiles (divided by 2).</p

Heritabilities of telomere length (kb) in sand lizards.

<p>In (a) males, the heritability between sons and sires was 1.23 (from a regression coefficient of 0.62±0.11, SE, n = 40). In (b) females, the heritability between dams and daughters was 0.52 (from a regression coefficient of 0.26±0.11, n = 55).</p

Results from a multiple regression analysis examining the effects of age-independent telomere length, mean offspring sex ratio through a sire's lifetime, and malformation frequency on average of a male's offspring sired through his lifetime.

<p><u>Model statistics</u>:<i>F</i>_{3, 58} = 8.46, <i>P</i><0.0001, <i>R</i>² = 0.30.</p