Liquid-Phase Packaging of a Glucose Oxidase Solution with Parylene Direct Encapsulation and an Ultraviolet Curing Adhesive Cover for Glucose Sensors

We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film using a UV-curable adhesive and photolithography. The package was processed at room temperature to avoid denaturation of the glucose oxidase. The glucose oxidase solution was encapsulated and unsealed. Glucose sensing was demonstrated using standard amperometric detection at glucose concentrations between 0.1 and 100 mM, which covers the glucose concentration range of diabetic patients. Our proposed Parylene encapsulation and UV-adhesive cover form a liquid phase glucose-oxidase package that has the advantages of room temperature processing and direct liquid encapsulation of a small volume solution without use of conventional solidifying chemicals

短時間虚血再灌流刺激におけるウサギ心筋βアドレナリン性情報伝達

本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである京都大学0048新制・課程博士博士(医学)甲第5551号医博第1521号新制||医||576(附属図書館)UT51-94-C9京都大学大学院医学研究科内科系専攻(主査)教授 北 徹, 教授 眞崎 知生, 教授 篠山 重威学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

814-2 Responses of Internal Mammary Artery to Local Administration of Acetylcholine

Internal mammary arteries (IMA) are widely used for coronary artery bypass grafting, and being reported to have a much higher long-term patency rate than that of saphenous veins. Therefore, we hypothesized that the endothelial function of IMA is better preserved compared with that of coronary arteries. To evaluate the endothelial function of IMA, acetylcholine (ACh) was selectively injected into IMA and both coronary arteries in 7 male and 4 female patients (mean age; 61±11 (SD) years) with atypical chest pain and normal coronary arteriograms. Angiography was repeated before and after the selective injection of various doses of ACh and 1mg of isosorbide dinitrate (ISDN). Injection of ACh into coronary arteries provoked neither chest pain nor ST-segment shifts. The diameter of IMA and both coronary arteries was measured with a computer-assisted analysis system. Arterial pressures and heart rates remained unchanged despite the selective injection of ACh and ISDN. Diameters (mm) were:ControlAChISDN25/μg50/μg100/μgRCA2.04±0.491.87±0.40**1.62±0.41**–2.44±0.63**LCA2.06±0.501.92±0.50*1.80±0.49**1.69±0.46**2.37±0.54**IMA2.02±0.532.22±0.56**2.36±0.57**–2.43±0.50***p<0.05**p<0.01 vs. control values by ANOVAThe diameters of both coronary arteries were decreased by ACh in a dosedependent manner, whereas IMA showed a dose-dependent dilation with ACh. These findings indicate that the endothelial function of IMA is well preserved, because ACh-induced endothelium-derived relaxing factors could counteract the contraction of vascular smooth muscle cells induced by ACh