DRG-targeted helper-dependent adenoviruses mediate selective gene delivery for therapeutic rescue of sensory neuronopathies in mice

Dorsal root ganglion (DRG) neuron dysfunction occurs in a variety of sensory neuronopathies for which there are currently no satisfactory treatments. Here we describe the development of a strategy to target therapeutic genes to DRG neurons for the treatment of these disorders. We genetically modified an adenovirus (Ad) to generate a helper virus (HV) that was detargeted for native adenoviral tropism and contained DRG homing peptides in the adenoviral capsid fiber protein; we used this HV to generate DRG-targeted helper-dependent Ad (HDAd). In mice, intrathecal injection of this HDAd produced a 100-fold higher transduction of DRG neurons and a markedly attenuated inflammatory response compared with unmodified HDAd. We also injected HDAd encoding the β subunit of β-hexosaminidase (Hexb) into Hexb-deficient mice, a model of the neuronopathy Sandhoff disease. Delivery of the DRG-targeted HDAd reinstated neuron-specific Hexb production, reversed gangliosidosis, and ameliorated peripheral sensory dysfunction. The development of DRG neuron–targeted HDAd with proven efficacy in a preclinical model may have implications for the treatment of sensory neuronopathies of diverse etiologies

Precessional motion of a vortex in a finite-temperature Bose-Einstein condensate

We study the precessing motion of a vortex in a Bose-Einstein condensate of atomic gases. In addition to the former zero-temperature studies, finite temperature systems are treated within the Popov and semiclassical approximations. Precessing vortices are discussed utilizing the rotating frame of reference. The relationship between the sign of the lowest excitation energy and the direction of precession is discussed in detail.Comment: 6 pages, 9 figures. More discussion in Sec.III. Reference is update

First-principles study on scanning tunneling microscopy images of hydrogen-terminated Si(110) surfaces

Scanning tunneling microscopy images of hydrogen-terminated Si(110) surfaces are studied using first-principles calculations. Our results show that the calculated filled-state images and local density of states are consistent with recent experimental results, and the empty-state images appear significantly different from the filled-state ones. To elucidate the origin of this difference, we examined in detail the local density of states, which affects the images, and found that the bonding and antibonding states of surface silicon atoms largely affect the difference between the filled- and empty-state images.Comment: 4 pages, and 4 figure

A Molecular Line Observation toward Massive Clumps Associated with Infrared Dark Clouds

We have surveyed the N2H+ J=1-0, HC3N J=5-4, CCS J_N=4_3-3_2, NH3 (J, K) = (1, 1), (2, 2), (3, 3), and CH3OH J=7-6 lines toward the 55 massive clumps associated with infrared dark clouds by using the Nobeyama Radio Observatory 45 m telescope and the Atacama Submillimeter Telescope Experiment 10 m telescope. The N2H+, HC3N, and NH3 lines are detected toward most of the objects. On the other hand, the CCS emission is detected toward none of the objects. The [CCS]/[N2H+] ratios are found to be mostly lower than unity even in the Spitzer 24 micron dark objects. This suggests that most of the massive clumps are chemically more evolved than the low-mass starless cores. The CH3OH emission is detected toward 18 out of 55 objects. All the CH3OH-detected objects are associated with the Spitzer 24 micron sources, suggesting that star formation has already started in all the CH3OH-detected objects. The velocity widths of the CH3OH J_K=7_0-6_0 A+ and 7_{-1}-6_{-1} E lines are broader than those of N2H+ J=1-0. The CH3OH J_K=7_0-6_0 A+ and 7_{-1}-6_{-1} E lines tend to have broader linewidth in the MSX dark objects than in the others, the former being younger or less luminous than the latter. The origin of the broad emission is discussed in terms of the interaction between an outflow and an ambient cloud.Comment: Accepted to Ap

Mapping Observations of DNC and HN^13C in Dark Cloud Cores

We present results of mapping observations of the DNC, HN^13C, and H^13CO^+ lines (J=1-0) toward 4 nearby dark cloud cores, TMC-1, L1512, L1544, and L63, along with observations of the DNC and HN^13C lines (J=2-1) toward selected positions. By use of statistical equilibrium calculations based on the LVG model, the H_2 densities are derived to be (1.4-5.5)*10^5 cm^-3, and the [DNC]/[HN^13C] ratios are derived to be 1.25-5.44 with a typical uncertainty by a factor of 2. The observed [DNC]/[HNC] ratios range from 0.02 to 0.09, assuming the [^12C]/[^13C] ratio of 60. Distributions of DNC and HN^13C are generally similar to each other, whereas the distribution of H^13CO^+ is more extended than those of DNC and HN^13C, indicating that they reside in an inner part of the cores than HCO^+. The [DNC]/[HN^13C] ratio is rather constant within each core, although a small systematic gradients are observed in TMC-1 and L63. Particularly, no such systematic gradient is found in L1512 and L1544, where a significant effect of depletion of molecules is reported toward the central part of the cores. This suggests that the [DNC]/[HNC] ratio would not be very sensitive to depletion factor, unlike the [DCO^+]/[HCO^+] ratio. On the other hand, the core to core variation of the [DNC]/[HNC] ratio, which range an order of magnitude, is more remarkable than the variation within each core. These results are interpreted qualitatively by a combination of three competing time-dependent processes; gas-phase deuterium fractionation, depletion of molecules onto grain surface, and dynamical evolution of a core.Comment: 22 pages, 8 EPS figures, aasLaTex 5.0, accepted to The Astrophysical Journa

Molecular Line Observations of a Carbon-Chain-Rich Core L492

We report on molecular abundances and distributions in a starless dense core L492. We have found that the abundances of carbon-chain molecules such as CCS, C$_{3}$S, HC$_{3}$N, HC$_{5}$N, and HC$_{7}$N are comparable to those in chemically young dark cloud cores called "carbon-chain--producing regions", such as L1495B, L1521B, L1521E, and TMC-1. This is the first dark cloud core with extremely rich in carbon-chain-molecules that is found outside the Taurus region. In addition, the deuterium fractionation ratios of DNC/HNC and DCO$^{+}$/HCO$^{+}$ are also comparable to those in carbon-chain--producing regions, being significantly lower than those in the evolved prestellar cores such as L1498 and L1544. On the other hand, the abundances of NH$_{3}$ and N$_{2}$H$^{+}$ are systematically higher than those in carbon-chain--producing regions. Our mapping observations reveal that the central hole of molecular distributions, which were reported for CCS and C$^{34}$S in evolved prestellar cores is not significant in L492, indicating that the depletion factor of molecules is not very high. Furthermore, L492 is dynamically more evolved than carbon-chain--producing regions, and the protostellar collapse has started like L1498 and L1544. Therefore, it is likely that the chemical and dynamical evolutionary stage of L492 is intermediate between carbon-chain--producing regions (L1495B, L1521B, L1521E, and TMC-1) and evolved prestellar cores (L1498 and L1544).Comment: 26 pages, 8 figures, accepted for publication in The Astrophysical Journal (Preprint with high resolution figures will be available at http://www.nro.nao.ac.jp/library/report/list.html

Spin domain formation in spinor Bose-Einstein condensation

The spatial structure of the spinor Bose-Einstein condensates with the spin degrees of freedom is analyzed based on the generalized Gross-Pitaevskii equation (GP) in the light of the present spin domain experiment on m_F=\pm 1, and 0 of the hyperfine state F=1 of ^{23}Na atom gases. The GP solutions in three- and one-spatial dimensional cases reproduce the observed spin domain structures, revealing the length scale associated with the existence of the weak interaction of the spin-spin channel, other than the ordinary coherence length related to the density-density channel. The obtained domain structure in GP is compared with the result in Thomas-Fermi approximation. The former solution is found to better describe the observed features than the latter.Comment: 9 pages, 14 figure

Estimation of Power Balance in Steady State LHCD Discharges on TRIAM-1M

On TRIAM-1M, a long duration discharge for more than 5 h was achieved successfully by fully non-inductive lower hybrid current drive. Heat load distribution to the plasma facing components (PFCs) in the 5 h discharge was investigated by using calorimetric measurements. The injected RF power was coincident with the total amount of heat load to PFCs estimated by calorimetric measurement. The power balance including the portion of direct loss power of fast electron, heat flux due to the charge exchange process was able to be estimated in this long duration discharge.Comment: 12th International Congress on Plasma Physics, 25-29 October 2004, Nice (France

First-Principles Study on Peierls Instability in Infinite Single-Row Al Wires

We present the relation between the atomic and spin-electronic structures of infinite single-row atomic wires made of Al atoms during their elongation using first-principles molecular-dynamics simulations. Our study reveals that the Peierls transition indeed occurs in the wire with magnetic ordering: it ruptures to form a trimerized structure with antiferromagnetic ordering and changes from a conductor to an insulator just before forming a linear wire of equally-spaced atoms. The formation of the trimerized wire is discussed in terms of the behavior of the $\sigma$-symmetry bands of the Al wire.Comment: 10 pages, 4 figure

First-principles study of electron transport through C20C_{20} cages

Electron transport properties of C$_{20}$ molecules suspended between gold electrodes are investigated using first-principles calculations. Our study reveals that the conductances are quite sensitive to the number of C$_{20}$ molecules between electrodes: the conductances of C$_{20}$ monomers are near 1 G$_{0}$, while those of dimers are markedly smaller, since incident electrons easily pass the C$_{20}$ molecules and are predominantly scattered at the C$_{20}$-C$_{20}$ junctions. Moreover, we find both channel currents locally circulating the outermost carbon atoms.Comment: 8 pages and 3 figure