THE INHIBITORY EFFECTS OF MgSO_4 ON THE MULTIPLICATION AND TRANSCRIPTION OF MOUSE HEPATITIS VIRUS

The multiplication of mouse hepatitis virus (MHV) was inhibited by the treatment of infected cells with MgSO_4 at concentrations higher than 50 mM. The inhibition of viral multiplication was more effective with the treatment of cells at the early stage of infection using MgSO_4 than at the late stage. Viral adsorption to the cells was not inhibited by MgSO_4 and pretreatment of the cells with MgSO_4 did not show an inhibitory effect on the RNA synthesis of MHV. The synthesis of viral RNA was inhibited more effectively by the treatment of infected cells with MgSO_4 at 0-2 and 2-4 h postinfection (p.i.) than at 4-6 h p.i. The present study suggests that the stage at which viral multiplication is susceptible to MgSO_4 may be the early stage of viral transcription and that Mg^ may be a useful tool for the analysis of the early stage of MHV infection

Improvement of Cardiac Function after Granulocyte-colony Stimulating Factor-mobilized Peripheral Blood Mononuclear Cell Implantation in a Patient with Non-ischemic Dilated Cardiomyopathy Associated with Thromboangiitis Obliterans

Cardiac involvement is a rare complication with thromboangiitis obliterans (TAO). We report a 29-year-old man with TAO accompanied with non-ischemic dilated cardiomyopathy. He had no history of heart disease, but echocardiogram demonstrated diffuse hypokinesis and dilated left ventricle. Coronary angiography revealed no organic stenotic lesion. For limb salvage, he was treated with granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cell (PBMNC) implantation on his right leg. Not only ischemic leg symptoms, but also plasma level of BNP and ^I-metaiodobenzylguanidine scintigraphic parameters improved after 24 weeks. G-CSF-mobilized PBMNC implantation could be an effective approach to treating non-ischemic cardiomyopathy