MOESM1 of Genetic and physiological basis for antibody production by Kluyveromyces marxianus

Additional file 1: Table S1. Primers used in this study. Figure S1. Amino acid sequence of scFv. Figure S2. Sequence of codon optimized scFv fragment. Figure S3. Sequence of E02-014 plasmid. Figure S4. Sequence of KmPMDH1. Figure S5. Sequence of KmPACO1. Figure S6. Secreted scFv activity per cell amount

MOESM1 of Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae

Additional file 1: Table S1. Primer sequences, restriction sites, and DNA templates used for plasmid constructions in this study are summarized

MOESM1 of Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of d-glucose and l-arabinose

Additional file 1: Table S1. Specific growth rates and specific sugar consumption rates by recombinant strains and the wild-type strain of C. glutamicum ATCC 31831. Table S2. Relative expression levels of genes in the ara cluster and central metabolic pathway in aerobically grown wild-type and araR-deletion mutants with/without pyk overexpression (ΔaraR and ΔaraR/pCHpyk). Fig. S1. Retention time comparison of liquid chromatography–mass spectrometry/mass spectrometry total ion current chromatograms of oxaloacetate (OXA) in C. glutamicum cells grown aerobically in BT medium containing sugar mixture of d-glucose and l-arabinose (15 g/L each). The authentic standards of OXA at the concentration of 2, 10, and 20 μM (a, b, c) as well as their identified counterparts in C. glutamicum cells of the wild-type (d), and recombinant strains of 31831/pCHpyk (e) and ΔaraR/pCHpyk (f). OXA was identified based on retention time in chromatography and its mass spectrum. Concentration of OXA was determined with peak area of its multiple reaction monitoring (MRM) transition (131.0>87.1) in LC-MS/MS. Fig. S2. Mass spectra of identified oxaloacetate (OXA) in C. glutamicum cells grown aerobically in BT medium containing sugar mixture of d-glucose and l-arabinose (15 g/L each). The authentic standards of OXA at the concentration of 2, 10, and 20 μM (A) as well as their identified counterparts in C. glutamicum cells of the wild-type (d), and recombinant strains of 31831/pCHpyk (e) and ΔaraR/pCHpyk (f). OXA was identified based on retention time in chromatography and its mass spectrum acquired by the targeted multiple reaction monitoring (MRM) transition (131.0>87.1). Table S3. Oligonucleotides used for qPCR in this study

Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in <i>Synechocystis</i> sp. PCC 6803

<div><p>We tested the hypothesis that inducing photosynthesis in cyanobacteria requires respiration. A mutant deficient in glycogen phosphorylase (∆GlgP) was prepared in <i>Synechocystis</i> sp. PCC 6803 to suppress respiration. The accumulated glycogen in ΔGlgP was 250–450% of that accumulated in wild type (WT). The rate of dark respiration in ΔGlgP was 25% of that in WT. In the dark, P700⁺ reduction was suppressed in ΔGlgP, and the rate corresponded to that in (2,5-dibromo-3-methyl-6-isopropyl-<i>p</i>-benzoquinone)-treated WT, supporting a lower respiration rate in ∆GlgP. Photosynthetic O₂-evolution rate reached a steady-state value much slower in ∆GlgP than in WT. This retardation was solved by addition of d-glucose. Furthermore, we found that the contents of Calvin cycle intermediates in ∆GlgP were lower than those in WT under dark conditions. These observations indicated that respiration provided the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis.</p></div

MOESM1 of Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

Additional file 1: Figure S1. Relative transcription levels of cellulolytic enzyme-encoding genes in recombinant yeast strains. Gene ACT1 was used as the internal standard. The relative transcription levels were shown normalized to the level observed in strain EG-D-CBHI-D, whose relative transcription level was defined as 1. For each strain, data are presented as the mean Âą SD from three independent experiments

MOESM2 of Combined cell-surface display- and secretion-based strategies for production of cellulosic ethanol with Saccharomyces cerevisiae

Additional file 2: Figure S2. Time-course profiles of cell growth using host strain BY4741 and recombinant yeast strains in YPD medium. Each strain was inoculated in YPD medium to an initial OD660 of 0.05 and then cultured aerobically at 30 °C, 150 rpm for 72 h. For each strain, data are presented as the mean ¹ SD from three independent experiments

MOESM1 of Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition

Additional file 1. Schematic diagram of glycogen extraction from A. platensis in the presence of lysozyme and CaCl2