What are the historical roots of the COVID-19 infodemic?:Lessons from the past

This scoping review explores the history of the term infodemic and its usefulness as a tool for public health policymaking. It presents the information-related problems the term has encompassed; historical research on these problems, which predate the term itself; and in-depth analyses of their iterations in three historical outbreaks with long-term significance for public health policy: the 1918 influenza pandemic, the beginning of the HIV/AIDS pandemic in the 1980s, and the 2003 outbreak of severe acute respiratory syndrome. Particular attention is paid to the characteristics of scientific practice that inadvertently contributed to the generation of misinformation, as well as other factors that played a role: historical legacies, persistent inequalities and a growing distrust of scientific authority. Historical perspective helps balance contemporary analyses of infodemics that focus too narrowly on the role of new social media in disseminating misinformation and disinformation. Insights derived from the historical record can also be useful to contemporary infodemic management

Quadratic Mixing of Radio Frequency Signals using Superconducting Quantum Interference Filters

The authors demonstrate quadratic mixing of weak time harmonic electromagnetic fields applied to Superconducting Quantum Interference Filters, manufactured from high-$T_{\mathrm{c}}$ grain boundary Josephson junctions and operated in active microcooler. The authors use the parabolic shape of the dip in the dc-voltage output around B=0 to mix \emph{quadratically} two external rf-signals, at frequencies $f_{\mathrm{1}}$ and $f_{\mathrm{2}}$ well below the Josephson frequency $f_{\mathrm{J}}$, and detect the corresponding mixing signal at $| {f_{1}-f_{2}}|$. Quadratic mixing takes also place when the SQIF is operated without magnetic shield. The experimental results are well described by a simple analytical model based on the adiabatic approximation.Comment: 3 pages, 3 figure

High performance magnetic field sensor based on Superconducting Quantum Interference Filters

We have developed an absolute magnetic field sensor using Superconducting Quantum Interference Filter (SQIF) made of high-T_c grain boundary Josephson junctions. The device shows the typical magnetic field dependent voltage response V(B), which is sharp delta-like dip in the vicinity of zero magnetic field. When the SQIF is cooled with magnetic shield, and then the shield is removed, the presence of the ambient magnetic field induces a shift of the dip position from B_0 ~ 0 to a value B ~ B_1, which is about the average value of the earth magnetic field, at our latitude. When the SQIF is cooled in the ambient field without shielding, the dip is first found at B ~ B_1, and the further shielding of the SQIF results in a shift of the dip towards B_0 ~ 0. The low hysteresis observed in the sequence of experiments (less than 5% of B_1) makes SQIFs suitable for high precision measurements of the absolute magnetic field. The experimental results are discussed in view of potential applications of high-T_c SQIFs in magnetometry.Comment: 4 pages, 2 figure

Dynamics of SNARE Assembly and Disassembly during Sperm Acrosomal Exocytosis

The dynamics of SNARE assembly and disassembly during membrane recognition and fusion is a central issue in intracellular trafficking and regulated secretion. Exocytosis of sperm's single vesicle—the acrosome—is a synchronized, all-or-nothing process that happens only once in the life of the cell and depends on activation of both the GTP-binding protein Rab3 and of neurotoxin-sensitive SNAREs. These characteristics make acrosomal exocytosis a unique mammalian model for the study of the different phases of the membrane fusion cascade. By using a functional assay and immunofluorescence techniques in combination with neurotoxins and a photosensitive Ca(2+) chelator we show that, in unactivated sperm, SNAREs are locked in heterotrimeric cis complexes. Upon Ca(2+) entry into the cytoplasm, Rab3 is activated and triggers NSF/α-SNAP-dependent disassembly of cis SNARE complexes. Monomeric SNAREs in the plasma membrane and the outer acrosomal membrane are then free to reassemble in loose trans complexes that are resistant to NSF/α-SNAP and differentially sensitive to cleavage by two vesicle-associated membrane protein (VAMP)–specific neurotoxins. Ca(2+) must be released from inside the acrosome to trigger the final steps of membrane fusion that require fully assembled trans SNARE complexes and synaptotagmin. Our results indicate that the unidirectional and sequential disassembly and assembly of SNARE complexes drive acrosomal exocytosis

α-SNAP Prevents Docking of the Acrosome during Sperm Exocytosis because It Sequesters Monomeric Syntaxin

α-SNAP has an essential role in membrane fusion that consists of bridging cis SNARE complexes to NSF. α-SNAP stimulates NSF, which releases itself, α-SNAP, and individual SNAREs that subsequently re-engage in the trans arrays indispensable for fusion. α-SNAP also binds monomeric syntaxin and NSF disengages the α-SNAP/syntaxin dimer. Here, we examine why recombinant α-SNAP blocks secretion in permeabilized human sperm despite the fact that the endogenous protein is essential for membrane fusion. The only mammalian organism with a genetically modified α-SNAP is the hyh mouse strain, which bears a M105I point mutation; males are subfertile due to defective sperm exocytosis. We report here that recombinant α-SNAP-M105I has greater affinity for the cytosolic portion of immunoprecipitated syntaxin than the wild type protein and in consequence NSF is less efficient in releasing the mutant. α-SNAP-M105I is a more potent sperm exocytosis blocker than the wild type and requires higher concentrations of NSF to rescue its effect. Unlike other fusion scenarios where SNAREs are subjected to an assembly/disassembly cycle, the fusion machinery in sperm is tuned so that SNAREs progress uni-directionally from a cis configuration in resting cells to monomeric and subsequently trans arrays in cells challenged with exocytosis inducers. By means of functional and indirect immunofluorescense assays, we show that recombinant α-SNAPs — wild type and M105I — inhibit exocytosis because they bind monomeric syntaxin and prevent this SNARE from assembling with its cognates in trans. Sequestration of free syntaxin impedes docking of the acrosome to the plasma membrane assessed by transmission electron microscopy. The N-terminal deletion mutant α-SNAP-(160–295), unable to bind syntaxin, affects neither docking nor secretion. The implications of this study are twofold: our findings explain the fertility defect of hyh mice and indicate that assembly of SNAREs in trans complexes is essential for docking

Effects of magnetic field on two-dimensional Superconducting Quantum Interference Filters

We present an experimental study of two-dimensional superconducting quantum interference filters (2D-SQIFs) in the presence of a magnetic field B. The dependences of the dc voltage on the applied magnetic field are characterized by a unique delta-like dip at B=0, which depends on the distribution of the areas of the individual loops, and on the bias current. The voltage span of the dip scales proportional to the number of rows simultaneously operating at the same working point. In addition, the voltage response of the 2D-SQIF is sensitive to a field gradient generated by a control line and superimposed to the homogeneous field coil. This feature opens the possibility to use 2D superconducting quantum interference filters as highly sensitive detectors of spatial gradients of magnetic field.Comment: 3 pages, 4 figures, submitted to AP

Observation of Spontaneous Brillouin Cooling

While radiation-pressure cooling is well known, the Brillouin scattering of light from sound is considered an acousto-optical amplification-only process. It was suggested that cooling could be possible in multi-resonance Brillouin systems when phonons experience lower damping than light. However, this regime was not accessible in traditional Brillouin systems since backscattering enforces high acoustical frequencies associated with high mechanical damping. Recently, forward Brillouin scattering in microcavities has allowed access to low-frequency acoustical modes where mechanical dissipation is lower than optical dissipation, in accordance with the requirements for cooling. Here we experimentally demonstrate cooling via such a forward Brillouin process in a microresonator. We show two regimes of operation for the Brillouin process: acoustical amplification as is traditional, but also for the first time, a Brillouin cooling regime. Cooling is mediated by an optical pump, and scattered light, that beat and electrostrictively attenuate the Brownian motion of the mechanical mode.Comment: Supplementary material include

Two tone response of radiofrequency signals using the voltage output of a Superconducting Quantum Interference Filter

In the presence of weak time harmonic electromagnetic fields, Superconducting Quantum Interference Filters (SQIFs) show the typical behavior of non linear mixers. The SQIFs are manufactured from high-T_c grain boundary Josephson junctions and operated in active microcooler. The dependence of dc voltage output V_dc vs. static external magnetic field B is non-periodic and consists of a well pronounced unique dip at zero field, with marginal side modulations at higher fields. We have successfully exploited the parabolic shape of the voltage dip around B=0 to mix quadratically two external time harmonic rf-signals, at frequencies f_1 and f_2 below the Josephson frequency f_J, and detect the corresponding mixing signal at f_1-f_2. When the mixing takes place on the SQIF current-voltage characteristics the component at 2f_2 - f_1 is present. The experiments suggest potential applications of a SQIF as a non-linear mixing device, capable to operate at frequencies from dc to few GHz with a large dynamic range.Comment: 10 pages, 3 Figures, submitted to J. Supercond. (as proceeding of the HTSHFF Symposium, June 2006, Cardiff

The Management of Bilateral Ureteric Injury following Radical Hysterectomy

Iatrogenic ureteric injury is a well-recognised complication of radical hysterectomy. Bilateral ureteric injuries are rare, but do pose a considerable reconstructive challenge. We searched a prospectively acquired departmental database of ureteric injuries to identify patients with bilateral ureteric injury following radical hysterectomy. Five patients suffered bilateral ureteric injury over a 6-year period. Initial placement of ureteric stents was attempted in all patients. Stents were placed retrogradely into 6 ureters and antegradely into 2 ureters. In 1 patient ureteric stents could not be placed and they underwent primary ureteric reimplantation. In the 4 patients in which stents were placed, 2 were managed with stents alone, 1 required ureteric reimplantation for a persistent ureterovaginal fistula, and 1 developed a recurrent stricture. No patient managed by ureteric stenting suffered deterioration in serum creatinine. We feel that ureteric stenting, when possible, offers a safe primary management of bilateral ureteric injury at radical hysterectomy