Searching relevant polymorphisms of CYP2B6 in HIV infected patients

Poster presented at the From Basic Sciences to Clinical Research - First International Congress of CiiEM. Egas Moniz, Caparica, Portugal, 27- 28 November 2015"The CYP2B6 belongs to the family of Cytochrome P450 enzymes that catalyze the metabolism of a wide variety of drugs, including the anti-retroviral EFV. The CYP2B6 gene, that has been mapped in the chromosome 19, is highly polymorphic and some SNP, namely 516G>T and 785A>G, are associated with decreased protein expression. These variants are related to phenotypes that are characterized as EFV poor metabolizers, and consequently to episodes of neurotoxicity.

A glimpse of climate change impact on C. Arabica L. and C. Canephora Pierre ex A. Froehner physiology: the combined effects of enhanced growth CO2 and temperature.

Summary: The effective impact of climate changes on the coffee plant physiology, promoted by enhanced air [CO2] and global warming remain to be fully elucidated through biological studies. Therefore, this work aims at linking important coffee physiological responses to environmental changes of enhanced growth [CO2] and temperature on genotypes from the two major producing species. Potted plants from C. arabica cv. IPR 108 and of C. canephora cv. Conilon Clone 153 were grown under environmental controlled conditions, either at 380 or 700 ?L CO2 L-1 air, for 1 year, without water, nutrient or root development restrictions. After that the temperature was gradually increased from 25/20 ºC (day/night) up to 42/34 ºC. The long-term impacts of enhanced growth [CO2] and enhanced temperature on the photosynthetic functioning were assessed at 25/20 ºC, 31/25 ºC, 37/30 ºC and 42/34 ºC, through leaf gas exchanges (rates of net photosynthesis, Pn, stomatal conductance, gs, transpiration, Tr, and photosynthetic capacity, Amax), instantaneous water use efficiency (iWUE), fluorescence parameters (photochemical efficiency of the photosystem II under dark, Fv/Fm, and light, Fv?/Fm?, conditions, as well as the photochemical, qP, and non-photochemical, NPQ, quenchings, and quantum yield of the linear electron transport, ?e), photosynthetic pigments (chlorophyll and carotenoids) and some molecules with antioxidant role (ascorbate and ?-tocopherol). The results showed that enhanced [CO2] stimulates photosynthetic functioning, without negative down-regulation. Minor impacts were found in the photochemical performance until 37 ºC, but extensive impacts were shown at 42 ºC, especially in IPR108. Remarkable was the finding that enhanced [CO2] preserved a higher functional status (Pn, Amax, Fo, Fv/Fm) at high temperatures (37 and 42 ºC), what seems quite relevant under the predicted climate changes and global warming scenarios

Thylakoid lipids changes may account for photosynthetic acclimation ability of two coffea species subjected to heat.

Summary: Coffee is one of the world?s most traded agricultural products, and its production could be threatened by global warming. The aim of this work was to evaluate the effects of heat on photosynthetic activity and thylakoid membrane lipid dynamics, on genotypes of the two major coffee producing species. Potted plants from C. arabica L. cv. IPR108 and C. canephora Pierre Ex A. Froehner cv. Conilon Clone 153 were grown for 1 year under controlled conditions of temperature (25/20ºC, day/night), irradiance (650-800 ?mol m-2 s-1), RH (75%), photoperiod (12 h), and 380 ?L CO2 L-1. Thereafter, temperature was gradually raised to 42/34ºC (0.5ºC/ day), with a 7 days stabilization step at 31, 37 and 42ºC. Studies focused modifications of thylakoid lipid composition and photosynthetic performance. In CL153 photosynthetic capacity (Amax) was not affected until 42ºC (40% reduction). In IPR108 it was reduced 35 and 57% at 37ºC at 42ºC, respectively. Thylakoid electron transport rate for photosystems (PS) I and II increased (ca. 10-25%) up to 37ºC in both genotypes. At 42ºC only IPR108 presented depressed activities on PSII (15%) and PSI (18%). Under 37 and 42ºC, CL153 plants presented digalactosyldiacylglycerol (DGDG) (ca. 42%) and monogalactosyldiacylglycerol (MGDG) (28-34%) increases, while IPR108 showed higher MGDG at all temperatures. In CL153 less unsaturated DGDG and phosphatidylglycerol (PG) along with stable or increased DGDG/MGDG ratio, may have contributed to sustain thylakoid electron flow at 37ºC and even 42ºC. IPR108 displayed a strong PG rise at all temperatures, in accordance with enhanced PSs activity

7th Drug hypersensitivity meeting: part two

No abstract availabl

A randomized, open-label study of conjugated equine estrogens plus medroxyprogesterone acetate versus tibolone: effects on symptom control, bleeding pattern, lipid profile and tolerability

Objective To compare the effects of continuous combined conjugated equine estrogens plus medroxyprogesterone acetate (CEE/MPA) with those of tibolone on symptom control, bleeding pattern, lipid profile and tolerability in postmenopausal women.Methods This was a randomized, open-label, parallel-group, multicenter study. Generally healthy postmenopausal women with an intact uterus and no contraindications to hormone replacement therapy (HRT) or tibolone were enrolled. Each subject was randomly assigned to receive CEE/MPA 0.625 mg-5.0 mg or tibolone 2.5 mg daily for 13 treatment cycles, each of 28 days.Results A total of 85 subjects were enrolled and received at least one dose of study medication, of which 76 (89.4%) subjects completed the study (n = 40, CEE/MPA; n = 363 tibolone). the incidence of postmenopausal symptoms decreased significantly over time in both treatment groups, compared with baseline, including significant decreases in the incidence of urogenital and sexual health symptoms. Significant differences in symptom control (other than hot flushes) were observed between treatment groups in a few different cycles for different symptoms, but no consistent or clinically significant trends were observed. No statistically significant differences in the incidence of bleeding were observed between treatment groups after cycle 4. Significant decreases in total cholesterol (5.6%) and low-density lipoprotein (LDL) cholesterol (7.5%) were observed at cycle 13, compared with baseline, in the CEE/MPA group, and significant decreases in high-density lipoprotein (HDL) cholesterol (8.5%) and triglycerides (13.7%) were observed at cycle 13, compared with baseline, in the tibolone group. Significant weight gain was observed at cycle 13 in the. tibolone group (3.05 kg), compared with the CEE/MPA group (0.96 kg). the incidences of adverse events were similar in both treatment groups.Conclusions Women treated with CEE/MPA or tibolone showed significant improvement of postmenopausal symptoms, including urogenital and sexual health symptoms, and had similar bleeding patterns after four cycles of therapy. CEE/MPA and tibolone each induced a different mix of changes in the lipid profile.Universidade Federal de São Paulo, BR-04024002 São Paulo, BrazilUniv Fed Bahia, BR-41170290 Salvador, BA, BrazilUniv Fed Rio de Janeiro, BR-21945 Rio de Janeiro, BrazilFac Med Sci Minas Gerais, Belo Horizonte, MG, BrazilPontifical Catholic Univ, Sorocaba, BrazilUniv Fed Rio Grande Norte, BR-59072970 Natal, RN, BrazilUniv Fed Paraiba, BR-58059900 Joao Pessoa, Paraiba, BrazilSanta Casa Misericordia, São Paulo, BrazilUniversidade Federal de São Paulo, EPM, BR-04024002 São Paulo, BrazilWeb of Scienc