Entropy and Barrier-Hopping Determine Conformational Viscoelasticity in Single Biomolecules

Biological macromolecules have complex and non-trivial energy landscapes, endowing them a unique conformational adaptability and diversity in function. Hence, understanding the processes of elasticity and dissipation at the nanoscale is important to molecular biology and also emerging fields such as nanotechnology. Here we analyse single molecule fluctuations in an atomic force microscope (AFM) experiment using a generic model of biopolymer viscoelasticity that importantly includes sources of local `internal' conformational dissipation. Comparing two biopolymers, dextran and cellulose, polysaccharides with and without the well-known `chair-to-boat' transition, reveals a signature of this simple conformational change as minima in both the elasticity and internal friction around a characteristic force. A calculation of two-state populations dynamics offers a simple explanation in terms of an elasticity driven by the entropy, and friction by barrier-controlled hopping, of populations on a landscape. The microscopic model, allows quantitative mapping of features of the energy landscape, revealing unexpectedly slow dynamics, suggestive of an underlying roughness to the free energy.Comment: 25 pages, 7 figures, naturemag.bst, modified nature.cls (naturemodified.cls

Modeling the Effects of Human Activity on Katmai Brown Bears (Ursus arctos) through the Use of Survival Analysis

Brown bear-human interactions were observed in 1993, 1995, and 1997 at Kulik River in Katmai National Park and Preserve, Alaska. We analyzed these interactions using survival analysis, creating survival curves for the time that bears remained on the river in the presence, and absence, of human activity. Bear-only survival curves did not vary significantly between years (p = 0.067). Ninety-seven percent of bears left the river within 70 minutes of arrival in all years. Temporal patterns of bear activity were unaffected by the presence of humans as long as the bears did not share river zones with humans (p = 0.062 to p = 0.360). When people and bears did not share river zones, 38.6% (1993), 36.0% (1995), and 37.0% (1997) of bears remained on the river for at least 10 minutes after arrival. In contrast, when people and bears shared river zones, fewer bears remained on the river after the first 10 minutes, with 28.6% (1993), 25.0% (1995), and 32.6% (1997) observed in each year. We conclude that human activity displaced 26.0% (1993), 30.5% (1995), and 12.0% (1997) of the bears using the river, which otherwise would likely have remained longer. Over the three years of study, habituation to human activity may account for observed changes in bears' use of the river.En 1993, 1995 et 1997, des interactions ours brun-être humain ont fait l'objet d'observations à la rivière Kulik, dans la réserve de parc national Katmai, en Alaska. On a analysé ces interactions en ayant recours à l'analyse de survie, créant des courbes de survie pour la durée où les ours restaient à la rivière en présence et en l'absence d'activité humaine. Les courbes de survie pour l'ours seul ne variaient pas sensiblement d'une année à l'autre (p = 0,067). Dans tous les cas, 97% des ours quittaient la rivière dans les 70 minutes suivant leur arrivée. Les schémas de comportement temporel des ours n'étaient pas affectés par la présence d'êtres humains tant que les ours ne partageaient pas les mêmes zones de rivière que les humains (p = 0,062 à p = 0,360). Quand individus et ours ne partageaient pas les mêmes zones de rivière, 38,6% (1993), 36,0% (1995) et 37,0% (1997) des ours restaient à la rivière au moins 10 minutes après leur arrivée. En revanche, quand individus et ours partageaient les zones de rivière, moins d'ours restaient à la rivière au-delà des 10 premières minutes, 28,6% (1993), 25,0% (1995) et 32,6% (1997) ayant été observés chaque année. On conclut que l'activité humaine a délogé 26,0% (1993), 30,5% (1995) et 12,0% (1997) des ours utilisant la rivière, qui, autrement, seraient restés plus longtemps. Au cours des trois années de l'étude, il se pourrait que l'accoutumance à l'activité humaine explique les changements que l'on a observés dans l'utilisation de la rivière par les ours

Aeroacoustic performance of a seal vibrissa shaped cylinder

Bio-inspired geometries have many applications in engineering, including in the field of noise control. In this work, the aeroacoustic performance of a seal vibrissa shaped cylinder (SVSC) is investigated and compared to that of a circular cylinder at Re = 37 000. Experiments conducted in an anechoic wind tunnel are compared to results from a hybrid aeroacoustic simulation with excellent agreement observed between the two. The overall sound pressure level is found to be 24.3 dB lower for the SVSC, and no prominent narrowband component is observed in the acoustic spectrum. Analysis of the flow field and surface pressure fluctuations reveals that this is because the usual large-scale alternating vortex shedding realized for bluff body flows is absent for the SVSC. Instead, smaller uncorrelated vortices are shed from the upper and lower sides of the geometry, which, when combined with a lower spanwise correlation, results in a much lower acoustic intensity spread over a broader frequency range

Strength is negatively associated with depression and accounts for some of the sex difference A replication and extension

Background Depression occurs about twice as often in women as in men, a disparity that remains poorly understood. In a previous publication, Hagen and Rosenstrom predicted and found that grip strength, a highly sexually dimorphic index of physical formidability, mediated much of the effect of sex on depression. Striking results like this are more likely to be published than null results, potentially biasing the scientific record. It is therefore critical to replicate and extend them. Methodology Using new data from the 2013-14 cycle of the National Health and Nutrition Examination Survey, a nationally representative sample of US households (n = 3650), we replicated models of the effect of sex and grip strength on depression reported in Hagen and Rosenstrom, along with additional potential confounds and a new detailed symptom-level exploration. Results Overall, the effects from the original paper were reproduced although with smaller effect sizes. Grip strength mediated 38% of the effect of sex on depression, compared to 63% in Hagen and Rosenstrom. These results were extended with findings that grip strength had a stronger association with some depression symptoms, like suicidality, low interest and low mood than with other symptoms, like appetite changes. Conclusions Grip strength is negatively associated with depression, especially its cognitive-affective symptoms, controlling for numerous possible confounds. Although many factors influence depression, few of these reliably occur cross-culturally in a sex-stratified manner and so are unlikely to explain the well-established, cross-cultural sex difference in depression. The sex difference in upper body strength occurs in all populations and is therefore a candidate evolutionary explanation for some of the sex difference in depression. Lay summary: Why are women at twice the risk of developing depression as men? Depression typically occurs during social conflicts, such as physical or sexual abuse. Physically strong individuals can often single-handedly resolve conflicts in their favor, whereas physically weaker individuals often need help from others. We argue that depression is a credible cry for help. Because men generally have greater strength than women, we argue that men may be more likely to resolve conflicts using physical formidability and women to signal others for help. We find that higher grip strength is associated with lower depression, particularly symptoms like feeling down or thoughts of suicide and that strength accounts for part of the sex difference in rates of depression.Peer reviewe

Current oscillations in Vanadium Dioxide: evidence for electrically triggered percolation avalanches

In this work, we experimentally and theoretically explore voltage controlled oscillations occurring in micro-beams of vanadium dioxide. These oscillations are a result of the reversible insulator to metal phase transition in vanadium dioxide. Examining the structure of the observed oscillations in detail, we propose a modified percolative-avalanche model which allows for voltage-triggering. This model captures the periodicity and waveshape of the oscillations as well as several other key features. Importantly, our modeling shows that while temperature plays a critical role in the vanadium dioxide phase transition, electrically induced heating cannot act as the primary instigator of the oscillations in this configuration. This realization leads us to identify electric field as the most likely candidate for driving the phase transition

An Untriggered Search for Optical Bursts

We present an untriggered search for optical bursts with the ROTSE-I telephoto array. Observations were taken which monitor an effective 256 square degree field continuously over 125 hours to m_{ROTSE}=15.7. The uniquely large field, moderate limiting magnitude and fast cadence of $\sim$10 minutes permits transient searches in a new region of sensitivity. Our search reveals no candidate events. To quantify this result, we simulate potential optical bursts with peak magnitude, m_{p}, at t=10 s, which fade as f=(\frac{t}{t_{0}}) ^{\alpha_{t}}, where \alpha_t < 0. Simple estimates based on observational evidence indicate that a search of this sensitivity begins to probe the possible region occupied by GRB orphan afterglows. Our observing protocol and image sensitivity result in a broad region of high detection efficiency for light curves to the bright and slowly varying side of a boundary running from [\alpha_{t},m_{p}]=[-2.0,6.0] to [-0.3,13.2]. Within this region, the integrated rate of brief optical bursts is less than 1.1\times 10^{-8} {\rm s}^{-1} {\rm deg}^{-2}. At $\sim$22 times the observed GRB rate from BATSE, this suggests a limit on \frac{\theta_{opt}}{\theta_{\gamma}}\lesssim 5 where \theta_{opt} and \theta_{\gamma} are the optical and gamma-ray collimation angles, respectively. Several effects might explain the absence of optical bursts, and a search of the kind described here but more sensitive by about 4 magnitudes should offer a more definitive probe.Comment: 8 pages, 6 figures, 1 tabl

Spatially targeted nature-based solutions can mitigate climate change and nature loss but require a systems approach

Funding Information: This study was funded by the Royal Society for the Protection of Birds (RSPB) and Natural England (project code ECM 58632). The Breeding Bird Survey is a Partnership between the BTO, RSPB, and Joint Nature Conservation Committee (on behalf of Natural Resources Wales, Natural England, Council for Nature Conservation and Countryside, and NatureScot) and relies on volunteer surveyors. Simon Gillings provided tetrad-level predictions of relative abundance for wading birds. We are grateful to members of the RSPB steering group, who contributed to the development of our scenarios, and Profs. Tim Benton and Andrew Balmford who commented on an earlier version of this manuscript. Conceptualization, T.F. R.B.B. T.B.-L. G.M.B. W.J.P. and R.H.F.; methodology, T.F. T.B.-L. J.P.C. D.M. P.S. and R.H.F.; software, T.F.; formal analysis, T.F.; resources, D.M.; data curation, T.F.; writing – original draft, T.F.; writing – review & editing, R.B.B. T.B.-L. G.M.B. J.P.C. D.M. P.S. W.J.P. and R.H.F.; visualization, T.F.; supervision, W.J.P. The authors declare no competing interests. Publisher Copyright: © 2023 The AuthorsPeer reviewedPublisher PD

Galaxy populations in the Antlia cluster. I. Photometric properties of early-type galaxies

We present the first colour-magnitude relation (CMR) of early-type galaxies in the central region of the Antlia cluster, obtained from CCD wide-field photometry in the Washington photometric system. Integrated (C -T1) colours, T1 magnitudes, and effective radii have been measured for 93 galaxies (i.e. the largest galaxies sample in the Washington system till now) from the FS90 catalogue (Ferguson & Sandage 1990). Membership of 37 objects can be confirmed through new radial velocities and data collected from the literature. The resulting colour-magnitude diagram shows that early-type FS90 galaxies that are spectroscopically confirmed Antlia members or that were considered as definite members by FS90, follow a well defined CMR (sigma_(C -T1) ~ 0.07 mag) that spans 9 magnitudes in brightness with no apparent change of slope. This relation is very tight for the whole magnitude range but S0 galaxies show a larger dispersion, apparently due to a separation of ellipticals and S0s. Antlia displays a slope of -13.6 in a T1 vs. (C -T1) diagram, in agreement with results for clusters like Fornax, Virgo, Perseus and Coma, which are dynamically different to Antlia. This fact might indicate that the build up of the CMR in cluster of galaxies is more related to galaxies internal processes than to the influence of the environment. Interpreting the CMR as a luminosity-metallicity relation of old stellar systems, the metallicities of the Antlia galaxies define a global relation down to Mv ~ -13. We also find, for early-type dwarfs, no clear relation between luminosity and effective radius, indicating a nearly constant mean effective radius of ~ 1 kpc. This value is also found in several samples of dwarf galaxies in Virgo and Coma.Comment: 13 pages, 6 figures. Accepted for publication in MNRA