The effect of feeding wheat with purple pericarp on the growth of carp

This study assessed and compared the influence of feeding wheat with purple pericarp (variety Konini) and standard coloured wheat (red variety Bohemia) on the growth characteristics of fingerling carp (Cyprinus carpio L.) of the "Amurský lysec" line. The total content of anthocyanins converted to cyanidin 3-glucoside in the control Bohemia wheat was 24.95 mg.kg-1 and in the Konini purple wheat 41.70 mg.kg-1. Two experimental variants for feed were evaluated: dipped wheat grain and crushed wheat grain. The feed dose for wheat was 1.5% of the fish stock weight and for natural food (frozen Chironomid larvae) was 0.2% of fish stock weight to all variants. Growth parameters (body length, body weight, Fulton's condition factor and feed conversion ratio) of the fish were evaluated after one month of administration. The feed consumption and physico-chemical parameters (temperature, oxygen saturation, pH, N-NH4 +, N-NO2-, N-NO3- and Cl-) of the environment were observed. During the feeding test, no major differences in food consumption among variations feeding on either wheat and on Chironomid larvae were noted. Satisfying results for mas and length gain were achieved in V2 wheat with purple pericarp (Konini variety - dipped grain), where the average total body length was 156.56 mm and the average unit mass was 60.81 g. In this variant, higher values of the parameters were achieved compared to the control group (100.6%, resp. 104.2%). A positive impact of wheat with purple pericarp on the evaluated parameter of fish condition factor was demonstrated. This trend was confirmed in all variants. No effect was demonstrated for mechanical disruption of kernels on the level of utilization of nutrients. In further experiments on growth characteristics we would like to determine antioxidant parameters in the blood and liver of fry

Identifikacija alela gena za puroindolin i njihov utjecaj na teksturu zrna pšenice (Triticum aestivum L.)

Grain hardness is one of the most important quality characteristics of wheat (Triticum aestivum L.). It is a significant property of wheat grains and relates to milling quality and end product quality. Grain hardness is caused by the presence of puroindoline genes (Pina and Pinb). A collection of 25 genotypes of wheat with unusual grain colour (blue aleurone, purple and white pericarp, yellow endosperm) was studied by polymerase chain reaction (PCR) for the diversity within Pina and Pinb (alleles: Pina-D1a, Pina-D1b, Pinb-D1a, Pinb- -D1b, Pinb-D1c and Pinb-D1d). The endosperm structure was determined by a non-destructive method using light transfl ectance meter and grain hardness by a texture analyser. Genotype Novosibirskaya 67 and isogenic ANK lines revealed hitherto unknown alleles at the locus for the annealing of primers of Pinb-D1. Allele Pinb-D1c was found to be absent from each genotype. The mealy endosperm ranged from 0 to 100 % and grain hardness from 15.10 to 26.87 N per sample.Tvrdoća zrna jedno je od najvažnijih svojstava pšenice (Triticum aestivum L.) koje utječe na mlinarsku kakvoću i svojstva krajnjeg proizvoda, a određuju ju geni za puroindolin (Pina i Pinb). Metodom lančane reakcije polimeraze određena je prisutnost različitih alela gena Pina i Pinb (Pina-D1a, Pina-D1b, Pinb-D1a, Pinb-D1b, Pinb-D1c i Pinb-Did) u 25 genotipova pšenice neobične boje zrna (plavi aleuron, ljubičasti i bijeli perikarp, žuti endosperm), a nakon toga su ispitani struktura i tvrdoća zrna. U genotipu Novosibirskaya 67 i izogenoj liniji ANK otkriveni su dosad nepoznati aleli na mjestu sparivanja početnica gena Pinb-D1. Utvrđeno je da genotipovi nisu sadržavali alel Pinb-D1c. Udjel je brašnastog endosperma u uzorku zrna pšenice bio od 0 do 100 %, a tvrdoća zrna od 15,10 do 26,87 N

Testing of DNA isolation for the identification of hemp

Hemp is diploid organism (2n = 2x = 20, genome size 534 Mb) with nine pairs of autosomes plus XX (♀) or XY (♂) chromosomes. Cannabis sativa L. is an important economic plant for the production of food, fibre, oils, and intoxicants. Genotypes (varieties or chemovar) of hemp with low Δ9-tetrahydrocannabinol content are used for industrial applications. Varieties with high Δ9-tetrahydrocannabinol or high cannabidiol content are used for medicinal applications. Biochemical and molecular methods can be used for identification and classification. An important step for molecular biology methods is to obtain the matrix of the native and sufficiently pure DNA. We tested two different experimental variant of samples (20 mg and 100 mg) of seeds, oilcake and dried flowers for analysis of the Italian variety Carmagnola for analysis (harvested in 2014, Hempoint Ltd., Czech Republic). The DNeasy® Plant Mini Kit (Qiagen, GE) was used to isolate the DNA. The DNA concentration and purity was assessed by agarose electrophoresis and via a spectrophotometer. Samples of lower weight yielded lower values of DNA concentration (average 16.30 - 38.90 ng.µL-1), but with better purity than samples of higher weight (ratio A260nm/A280nm for low-weight samples was near 1.80). To test the applicability of DNA analysis, we used two SSR markers (CAN1347 and CAN2913). PCR products were separated on 1% agarose and on 8% polyacrylamide electrophoresis. DNA samples obtained from samples of higher weight exhibited less PCR amplification than samples of lower weight. We found no effect of sample weight on the formation of non-specific amplification products during the PCR reaction. Based on our results we can be recommended for practical isolation procedure using DNeasy® Plant Mini Kit with lower of sample weight (20 mg). In future work the procedure for DNA isolating from wheat-cannabis products, e. g. breads, rolls or pasta, will be optimized

Rheological and fermentation properties of doughs and quality of breads from colored wheat varieties

The objective of this study was to examine the rheological and fermentation behavior of doughs prepared from five different colored wheat varieties (black AF Zora, yellow KM 111-18, purple AF Jumiko, blue AF Oxana and red Vanessa - chosen as a standard), which contain polyphenolics in the outer layers of grains. Three wholemeal flour fractions (fine, semi-coarse and coarse) were used for each variety. The flour fractions differed in the particle size of the bran, the ash content and thus the phenolic compound content. The baking trials, texture and sensory analyses of breads were performed, to assess their overall acceptability.The coarser granulation of flour fractions, average hardness (8.587>77%), cohesiveness (78>75>70%) and resilience (35>32>27%) decreased. Moreover, the increase in off-flavors was detected with higher bran content. Regarding the flour granulation, the fine fraction seemed to be the most suitable due to its high gas-retention capacity. The best products in terms of both dough and bread quality reached blue AF Oxana and yellow KM 111-18.Utilization of colored wheat in bakery industry may present a good strategy of providing value-added products to the consumers.IGA/FT/2022/008, QK1910343; Ministerstvo ZemědělstvíInternal Grant Agency of Tomas Bata University in Zlin [IGA/FT/2022/008, QK1910343]; Ministry of Agriculture of the Czech Republi

Impact of Added Colored Wheat Bran on Bread Quality

The impact of colored wheat bran addition on bread quality was tested on wheat varieties with purple pericarp (Konini, Rosso and Karkulka) and on a variety containing blue aleurone (Skorpion). The effect of 10 %, 15 % and 20 % bran addition on sensory evaluation, bread color and texture was compared to the characteristics of bread prepared from wheat variety Mulan. The addition of 10 % bran significantly increased the sensory evaluation scores of bread. Crumb characteristics were improved mainly by the addition of 10 % bran. Moreover, top-quality bread contained bran separated from wheat variety Konini.O

The effect of feeding wheat varieties with different grain pigmentation on growth performance, texture, colour and meat sensory traits of broiler chickens

The feeding effect of of three spring wheat genotypes (Vánek, Konini and UC66049) with different grain colour on growth performance, body composition and meat quality parameters of broiler chickens was tested. Ninety chickens were divided into three groups (control, Konini and UC) with 30 chickens in each. The tested genotypes were compares with standard variety Vánek (control) with common (red) grain colour. The two experimental groups received feed mixtures containing 38.2% of wheats with different grain colour: groups Konini (n = 30) and UC (n = 30) with. The third group (n = 30) had 38.2% of common wheat Vánek cultivar (Control group). The live weight of chickens between the experimental groups and control group was not significant different, as well as body composition and chemical analysis of breast and thigh meat of chickens. The feeding of wheat with different grain colour had no effect on performance parameters of broiler chickens. Breast meat tenderness according to the Razor Blade Shear Force was higher in control group against experimental groups. The colour change was not significantly different in all coordinates. pH values (measured after 1-hour post mortem) were found significantly higher in the group fattening with Konini wheat than control and UC groups. Chickens meat from the experimental group was characterised by steady overall quality. The effect of various feeding had no effect on meat quality in terms of relevance to consumers

The effect of hempseed expellers on selected quality indicators of broiler chicken's meat

The aim of the study was to evaluate the effect of feeding hempseed expellers in a feed mixture on the quality indicators of broiler chicken's meat. One hundred and fifty Ross 308 hybrid cockerels were used in the present study. The control group (HS0) was fed without hempseed expellers; the other two groups received diets containing 50 g.kg-1 and 150 g.kg-1 of hempseed expellers (HS5 and HS15, respectively). The birds were slaughtered at the age of 37 days, and samples of breast and thigh muscles were collected for determination of proximate chemical composition and technological properties, and sensory analyses. Feeding with hempseed expellers influenced the colour of meat with a significant difference observed for a* (redness) and b* (yellowness) values in the HS15 group. The colour of breast meat in HS15 group is more intense compared to HS5 and HS0 groups. Breast meat was evaluated as the best in terms of odour for HS15 group compared to HS0. The colour of thigh meat was better rated in the HSE supplemented groups compared to the controls. In conclusion, dietary supplementation with hempseed expellers appears to affect the colour and odour of broiler chicken's meat which is positive for the consumers. Including hempseed cakes can be recommended as a component of broiler chicken's feed.O

Influence of feeding colored wheat varieties on selected quality parameters of broiler chicken’s meat

The effect of feeding colored wheat varieties (PS&nbsp;Karkulka and Skorpion) on chicken&acute;s performance and texture, color and sensory characteristics of broiler chicken&acute;s meat were evaluated in this study. The experiment was performed with 66 of Ross 308 cockerels. Cockerels were divided into three equal groups. The two experimental groups received feed mixtures containing 40% of wheats with different grain colour: groups PS Karkulka (n = 22) with PS&nbsp;Karkulka wheat cultivar and Skorpion (n = 22) with Skorpion cultivar. The third group (n = 22) had 40% of common wheat V&aacute;nek cultivar (Control). The live weight of broilers between all three groups was not significant different, as well as carcass yield and chemical composition of breast and thigh meat of chickens. In the parameter Razor Shear Force was found statistically significant higher breast meat tenderness in PS Karkulka against Control and Skorpion groups. In parameters L* and b* of colour of the meat samples was found statistically significant higher value in L* parameter in Skorpion group and b* parameter was higher in Control group. The total colour change was 2.25 and 2.53 for PS Karkulka and Skorpion group, respectively. In sensory analysis of broilers breast muscle was found statistically significant differences in odour, colour, fibreness, chewiness, juiciness and flavour parameter. The fatty taste parameter was non-significant. The odour parameter of chicken&acute;s breast muscle was significantly lower in Skorpion group against PS&nbsp;Karkulka and Control groups. The significantly most intense colour of breast muscle was found in Control group versus Skorpion and PS Karkulka groups (91.71 mm, 79.71 mm and 71.15 mm, respectively). The fibreness parameter was significantly higher for Control group, as well. Significantly higher chewiness of breast meat was in Control (68.49 mm) than PS Karkulka (52.02 mm) and Skorpion (43.32 mm) group. The feeding of wheat cultivars with different grain pigmentation had no effect on performance parameters of broiler chicken&acute;s as well as to it&acute;s body and chemical composition of breast and thigh meat in this study. 16.00 Normal 0 21 false false false EN-GB X-NONE X-NONE <w:LsdException Locked="false" Priority="61" Name="Light List